FastQCFastQC Report
Thu 26 May 2016
SRR1547752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547752_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2200554
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127050.5773546116114396No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37470.17027530340087088No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTC150.001309951180.06
CGTTTTT69450.075.507561
GCGTAAG2500.075.21
ACTACGG800.075.02
TGAGCGG4300.070.697672
GTCCGTT800.070.09
ACGGGAT7100.069.295785
GGACCGT4200.068.5714348
ACGCGTA351.1819975E-768.571431
TTGCGAG2050.068.292691
GCGCGAC1000.068.000019
TATAGGG33750.067.7925953
CGTTAGG4250.067.764712
CGAGGGA11200.067.54
CGCGGGT2650.066.415094
TTTGCGG11150.066.367712
AACGCGG2050.066.341462
TTTAGCG2300.066.086961
TGCGTAG1150.066.086961
CGAAGGG15350.065.6677553
Produced by FastQC (version 0.11.5)