FastQCFastQC Report
Thu 26 May 2016
SRR1547751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547751_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1079825
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63330.5864839210057186No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13400.12409418192762715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG304.0599843E-880.000012
GATCGAC150.001309769280.000019
GCGAACG304.0599843E-880.000011
GCGCGAC750.074.6666649
TCGATAG750.074.6666641
ACGGGTA700.074.285715
AGTAGCG950.071.578951
CGTATGG1400.071.428572
CGTTTTT36500.071.01371
TAGGGCG800.070.05
GGCGCGA800.070.08
GCGTAAG800.070.01
CGTAAGG1850.069.1891862
TAGGGAC12250.068.8979645
CTAAGCG1050.068.5714341
ATAGCCG650.067.6923180
TATCACG303.7954287E-666.666671
CGCGGGT303.7954287E-666.666674
AGGGCGA3600.065.555566
ACGGGAT5750.065.3913045
Produced by FastQC (version 0.11.5)