##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547751_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1079825
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.824107610029404	31.0	31.0	33.0	30.0	34.0
2	32.27219873590628	33.0	31.0	34.0	31.0	34.0
3	32.41436899497604	34.0	31.0	34.0	31.0	34.0
4	35.99330169240386	37.0	35.0	37.0	35.0	37.0
5	35.95341837797791	37.0	35.0	37.0	35.0	37.0
6	36.02447618827125	37.0	35.0	37.0	35.0	37.0
7	36.25142870372514	37.0	35.0	37.0	35.0	37.0
8	36.208600467668376	37.0	36.0	37.0	35.0	37.0
9	38.102306392239484	39.0	39.0	39.0	37.0	39.0
10-11	37.543940916352184	39.0	37.5	39.0	35.0	39.0
12-13	36.400268562035514	38.0	35.0	39.0	33.0	39.0
14-15	37.33031185608779	39.0	35.0	41.0	33.0	41.0
16-17	37.60915657629708	39.0	35.0	41.0	33.5	41.0
18-19	37.51987868404603	39.0	35.0	41.0	33.5	41.0
20-21	37.29243766351029	39.0	35.0	41.0	33.0	41.0
22-23	37.079391568078165	38.5	35.0	41.0	33.0	41.0
24-25	37.02853749450142	38.0	35.0	41.0	33.0	41.0
26-27	36.99910309540898	38.0	35.0	41.0	33.0	41.0
28-29	37.036260968212446	38.0	35.0	41.0	33.0	41.0
30-31	36.88760308383303	38.0	35.0	41.0	32.5	41.0
32-33	36.53281550251198	38.0	35.0	41.0	31.5	41.0
34-35	36.26113536915704	38.0	35.0	41.0	31.0	41.0
36-37	36.02821614613479	38.0	35.0	41.0	30.0	41.0
38-39	35.95426157016183	38.0	35.0	40.5	30.0	41.0
40-41	35.83998101544232	38.0	35.0	40.0	30.0	41.0
42-43	35.79636191049475	38.0	35.0	40.0	30.0	41.0
44-45	35.70321348366633	37.0	35.0	40.0	30.0	41.0
46-47	35.620368578241845	37.0	35.0	40.0	30.0	41.0
48-49	35.534876484615566	37.0	35.0	40.0	29.5	41.0
50-51	35.36039173014146	36.0	35.0	40.0	29.0	41.0
52-53	35.208940337554694	36.0	34.5	40.0	29.0	41.0
54-55	35.06013705924571	36.0	34.0	40.0	29.0	41.0
56-57	34.75846456601764	35.5	34.0	40.0	27.5	41.0
58-59	34.49297849188526	35.0	34.0	39.0	27.0	41.0
60-61	34.238692380709836	35.0	34.0	39.0	26.5	41.0
62-63	33.8389521450235	35.0	33.5	39.0	26.0	40.0
64-65	33.60473085916699	35.0	33.0	38.0	26.0	40.0
66-67	33.39468756511472	35.0	33.0	37.0	25.0	40.0
68-69	33.099358692380704	35.0	33.0	37.0	25.0	39.5
70-71	32.8937188896349	35.0	33.0	36.0	25.0	39.0
72-73	32.65437362535596	35.0	33.0	36.0	24.5	39.0
74-75	32.39582617553771	35.0	33.0	35.5	24.0	38.0
76-77	30.34379830065057	33.0	29.5	34.5	19.5	36.0
78-79	31.393568402287407	34.0	31.5	35.0	21.0	36.5
80-81	31.778302502720347	35.0	32.0	35.0	23.0	36.0
82-83	31.684085384205773	35.0	32.0	35.0	22.5	36.0
84-85	31.523058365938926	35.0	32.0	35.0	20.5	36.0
86	31.132567777186118	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	11.0
11	19.0
12	22.0
13	55.0
14	183.0
15	488.0
16	1209.0
17	2404.0
18	5017.0
19	7571.0
20	9229.0
21	9178.0
22	8274.0
23	7312.0
24	7632.0
25	8303.0
26	9826.0
27	12232.0
28	15128.0
29	18659.0
30	23348.0
31	29870.0
32	38428.0
33	54547.0
34	155070.0
35	131813.0
36	84942.0
37	145579.0
38	195756.0
39	97346.0
40	364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.0698261292339	24.376635102910193	29.507929525617577	14.045609242238324
2	29.95290903618642	26.524946171833395	30.16720301900771	13.354941772972474
3	29.481073322066077	24.769106105155927	33.11805153612854	12.631769036649457
4	26.97714907508161	26.613942073947168	32.51563910818883	13.893269742782396
5	24.985993100733918	31.31831546778413	29.11749589053782	14.578195540944133
6	22.42196652235316	41.04303938138124	25.858912323756165	10.676081772509434
7	88.30328988493505	3.7252332553886047	6.372514064779016	1.598962794897321
8	89.51580117148612	2.6949737225939385	6.098117750561434	1.6911073553585072
9	85.29604334035608	4.8342092468687055	7.483620031023546	2.3861273817516726
10-11	46.28092514990855	24.902229527932768	18.55745143889056	10.259393883268123
12-13	33.08624082605978	27.004236797629243	25.48556479059107	14.423957585719908
14-15	14.55434908434237	33.94888060565369	36.269163984904964	15.227606325098975
16-17	20.401546546894174	23.188942652744657	34.86574213414211	21.543768666219062
18-19	24.1864190956868	26.837496816613804	30.989975227467415	17.98610886023198
20-21	27.24520176880513	27.014979279049843	29.68823651980645	16.051582432338574
22-23	21.414858889171857	24.704373393836963	30.433357256962935	23.447410460028244
24-25	18.753594332414973	25.66897413932813	37.40096774940384	18.176463778853055
26-27	16.740814483828398	35.92785868080476	29.07860069918737	18.252726136179472
28-29	15.243442224434514	28.286527909614982	37.950177112032044	18.51985275391846
30-31	22.525270298427984	29.355914152756235	30.86004676683722	17.25876878197856
32-33	26.27143287106707	28.6931678744241	27.97189359386938	17.063505660639457
34-35	19.453105827333133	29.430602180908945	29.702590697566734	21.41370129419119
36-37	24.229944666959923	28.429097307434077	29.770518371032345	17.570439654573658
38-39	19.078832218183503	31.022619405922256	29.206352881253906	20.692195494640337
40-41	21.21112217257426	26.15854420855231	29.078091357395873	23.552242261477556
42-43	20.95608084643345	25.52723820989512	29.297478758132105	24.219202185539324
44-45	18.777255573819833	32.19757831130044	28.05482369828444	20.97034241659528
46-47	21.396337369481166	30.567175236728172	27.876878197856136	20.15960919593453
48-49	22.26138494663487	26.009075544648436	31.982774986687655	19.746764522029032
50-51	19.273539693931887	32.56013705924571	27.647257657490798	20.519065589331607
52-53	22.532308475910447	26.323107911004094	30.749056560090754	20.395527052994698
54-55	23.013590165073044	27.358136735119114	30.961452087143748	18.66682101266409
56-57	23.477693144722526	23.7297247239136	30.1327066885838	22.65987544278008
58-59	24.18109415877573	25.88813002106823	28.806473271131892	21.124302549024147
60-61	24.794434283332947	28.976176695297852	26.97520431551409	19.254184705855117
62-63	23.956103998333063	24.955201074248144	32.13187322019772	18.956821707221078
64-65	19.75032991456949	30.02449470979094	31.694163406107474	18.5310119695321
66-67	17.929618225175375	28.60093070636446	26.157895955363138	27.31155511309703
68-69	17.58780357928368	31.79357766304725	25.353784177991805	25.264834579677263
70-71	19.110689232051488	29.858773412358484	29.582756465167968	21.44778089042206
72-73	23.060820040284305	29.343088000370432	27.32530734146737	20.270784617877897
74-75	22.513694348621303	32.24490079411015	24.244113629523305	20.997291227745237
76-77	27.84224295603454	27.625263352858102	25.519181348829672	19.013312342277683
78-79	19.38735443243118	29.181302525872248	30.516657791771816	20.914685249924755
80-81	16.636028986178317	28.257912161692865	32.03028268469428	23.07577616743454
82-83	18.67089574699604	25.854374551431945	33.54650059037344	21.928229111198576
84-85	15.888546755261268	25.745468015650687	31.453800384321536	26.91218484476651
86	17.85881971615771	25.71444447016878	32.635751163382956	23.790984650290557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	666.0
1	895.0
2	2679.5
3	2768.0
4	1391.5
5	1697.5
6	2045.5
7	2178.0
8	2232.5
9	2255.0
10	2241.5
11	2231.0
12	2184.5
13	2221.0
14	2275.0
15	2519.5
16	2641.0
17	2570.0
18	2594.0
19	2727.5
20	2851.0
21	2813.0
22	3181.0
23	3905.5
24	4728.5
25	5799.5
26	7829.0
27	9664.5
28	10963.5
29	11852.0
30	14044.5
31	18209.5
32	20474.5
33	20147.5
34	21299.5
35	23646.0
36	24221.0
37	26610.5
38	29956.5
39	32854.5
40	39269.5
41	46287.5
42	54232.5
43	59633.0
44	61639.0
45	61525.0
46	65104.5
47	71417.0
48	66870.5
49	55935.5
50	50160.0
51	44147.5
52	33760.5
53	28130.5
54	25569.5
55	24924.5
56	24357.5
57	23130.0
58	20977.5
59	15641.5
60	11067.0
61	8966.5
62	6794.0
63	5034.5
64	4738.0
65	3757.5
66	2609.0
67	2175.5
68	1705.0
69	1242.0
70	814.0
71	644.0
72	576.5
73	368.0
74	215.5
75	141.0
76	54.0
77	35.0
78	39.0
79	38.0
80	20.5
81	4.0
82	4.0
83	3.5
84	4.5
85	3.5
86	2.0
87	3.0
88	2.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1079825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.805018423912042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.75025386143771	12.920642684395094
2	7.173263247167551	2.267471155621085
3	2.111210441321178	1.0010315976551007
4	0.9934539418130174	0.6280623141465113
5	0.6752980984538953	0.5336549443848291
6	0.4693607241433123	0.4450952935527449
7	0.33528490374945047	0.37094288567137484
8	0.2739390198519303	0.3463689004630532
9	0.2310114769068026	0.3286026583782432
>10	2.57580349990479	9.841464287211602
>50	1.332939258585116	15.405023728095768
>100	2.0711008400326505	54.615903836271094
>500	0.005900572193825215	0.5801620755541255
>1k	5.900572193825215E-4	0.12496659399494102
>5k	5.900572193825215E-4	0.5906070446044489
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6333	0.5864839210057186	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1340	0.12409418192762715	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8521519690690622E-4	0.0	0.0	0.03157919107262751	0.0
2	1.8521519690690622E-4	0.0	0.0	0.11585210566526984	0.0
3	1.8521519690690622E-4	0.0	0.0	0.16623063922394832	0.0
4	1.8521519690690622E-4	0.0	0.0	0.23753849003310723	0.0
5	1.8521519690690622E-4	0.0	0.0	0.38228416641585444	0.0
6	1.8521519690690622E-4	0.0	0.0	0.49387632255226543	0.0
7	1.8521519690690622E-4	0.0	0.0	0.5611094390294724	0.0
8	1.8521519690690622E-4	0.0	0.0	0.7001134443081055	0.0
9	1.8521519690690622E-4	0.0	0.0	0.7470654967240062	0.0
10	1.8521519690690622E-4	0.0	0.0	0.8513416525825944	0.0
11	1.8521519690690622E-4	0.0	0.0	1.0543375083925637	0.0
12	1.8521519690690622E-4	0.0	0.0	1.2092700206051907	0.0
13	1.8521519690690622E-4	0.0	0.0	1.2648345796772624	0.0
14	1.8521519690690622E-4	0.0	0.0	1.2909499224411363	0.0
15	1.8521519690690622E-4	0.0	0.0	1.3345681013127126	0.0
16	1.8521519690690622E-4	0.0	0.0	1.415136711967217	0.0
17	1.8521519690690622E-4	0.0	0.0	1.5241358553469313	0.0
18	1.8521519690690622E-4	0.0	0.0	1.6527678095987777	0.0
19	1.8521519690690622E-4	0.0	0.0	1.733984673442456	0.0
20	1.8521519690690622E-4	0.0	0.0	1.8268700946912695	0.0
21	1.8521519690690622E-4	0.0	0.0	1.9414256939781909	0.0
22	1.8521519690690622E-4	0.0	0.0	2.065519875905818	0.0
23	1.8521519690690622E-4	0.0	0.0	2.1875766906674694	0.0
24	1.8521519690690622E-4	0.0	0.0	2.283795985460607	0.0
25	1.8521519690690622E-4	0.0	0.0	2.365568494895006	0.0
26	1.8521519690690622E-4	0.0	0.0	2.4539161438196	0.0
27	1.8521519690690622E-4	0.0	0.0	2.545134628296252	0.0
28	1.8521519690690622E-4	0.0	0.0	2.6372791887574376	0.0
29	1.8521519690690622E-4	0.0	0.0	2.745722686546431	0.0
30	1.8521519690690622E-4	0.0	0.0	2.859815247841085	0.0
31	1.8521519690690622E-4	0.0	0.0	2.975019100317181	0.0
32	2.7782279536035933E-4	0.0	0.0	3.0717940407010396	0.0
33	3.7043039381381244E-4	0.0	0.0	3.1727363230153034	0.0
34	3.7043039381381244E-4	0.0	0.0	3.28581020072697	0.0
35	3.7043039381381244E-4	0.0	0.0	3.4147199777741766	0.0
36	3.7043039381381244E-4	0.0	0.0	3.532331627810062	0.0
37	3.7043039381381244E-4	0.0	0.0	3.6509619614289353	0.0
38	3.7043039381381244E-4	0.0	0.0	3.7845021183988146	0.0
39	3.7043039381381244E-4	0.0	0.0	3.9681429861320123	0.0
40	3.7043039381381244E-4	0.0	0.0	4.1092769661750745	0.0
41	3.7043039381381244E-4	0.0	0.0	4.2454101359016505	0.0
42	3.7043039381381244E-4	0.0	0.0	4.382191558817401	0.0
43	3.7043039381381244E-4	0.0	0.0	4.51304609543213	0.0
44	3.7043039381381244E-4	0.0	0.0	4.64686407519737	0.0
45	3.7043039381381244E-4	0.0	0.0	4.783830713310027	0.0
46	3.7043039381381244E-4	0.0	0.0	4.957192137614891	0.0
47	3.7043039381381244E-4	0.0	0.0	5.105364295140416	0.0
48	3.7043039381381244E-4	0.0	0.0	5.254555136248929	0.0
49	3.7043039381381244E-4	0.0	0.0	5.419211446299169	0.0
50	3.7043039381381244E-4	0.0	0.0	5.580626490403538	0.0
51	3.7043039381381244E-4	0.0	0.0	5.748246243604288	0.0
52	3.7043039381381244E-4	0.0	0.0	5.9372583520477855	0.0
53	3.7043039381381244E-4	0.0	0.0	6.1155279790706825	0.0
54	3.7043039381381244E-4	0.0	0.0	6.310189151019841	0.0
55	3.7043039381381244E-4	0.0	0.0	6.492440904776237	0.0
56	3.7043039381381244E-4	0.0	0.0	6.676637418100155	0.0
57	3.7043039381381244E-4	0.0	0.0	6.852221424767902	0.0
58	3.7043039381381244E-4	0.0	0.0	7.017711203204223	0.0
59	3.7043039381381244E-4	0.0	0.0	7.210057185192045	0.0
60	3.7043039381381244E-4	0.0	0.0	7.414349547380363	0.0
61	3.7043039381381244E-4	0.0	0.0	7.626235732641863	0.0
62	3.7043039381381244E-4	0.0	0.0	7.869052855786817	0.0
63	3.7043039381381244E-4	0.0	0.0	8.11881554881578	0.0
64	3.7043039381381244E-4	0.0	0.0	8.35505753247054	0.0
65	3.7043039381381244E-4	0.0	0.0	8.570370198874818	0.0
66	4.6303799226726555E-4	0.0	0.0	8.759567522515223	0.0
67	4.6303799226726555E-4	0.0	0.0	8.972564998958164	0.0
68	4.6303799226726555E-4	0.0	0.0	9.210196096589724	0.0
69	4.6303799226726555E-4	0.0	0.0	9.518579399439725	0.0
70	4.6303799226726555E-4	0.0	0.0	9.736855508994513	0.0
71	4.6303799226726555E-4	0.0	0.0	9.960873289653415	0.0
72	4.6303799226726555E-4	0.0	0.0	10.17525988007316	0.0
73	4.6303799226726555E-4	0.0	0.0	10.404648901442364	0.0
74	4.6303799226726555E-4	0.0	0.0	10.650522075336282	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	30	4.0599843E-8	80.00001	2
GATCGAC	15	0.0013097692	80.00001	9
GCGAACG	30	4.0599843E-8	80.00001	1
GCGCGAC	75	0.0	74.666664	9
TCGATAG	75	0.0	74.666664	1
ACGGGTA	70	0.0	74.28571	5
AGTAGCG	95	0.0	71.57895	1
CGTATGG	140	0.0	71.42857	2
CGTTTTT	3650	0.0	71.0137	1
TAGGGCG	80	0.0	70.0	5
GGCGCGA	80	0.0	70.0	8
GCGTAAG	80	0.0	70.0	1
CGTAAGG	185	0.0	69.189186	2
TAGGGAC	1225	0.0	68.897964	5
CTAAGCG	105	0.0	68.571434	1
ATAGCCG	65	0.0	67.69231	80
TATCACG	30	3.7954287E-6	66.66667	1
CGCGGGT	30	3.7954287E-6	66.66667	4
AGGGCGA	360	0.0	65.55556	6
ACGGGAT	575	0.0	65.391304	5
>>END_MODULE