FastQCFastQC Report
Thu 26 May 2016
SRR1547750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547750_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1796854
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98460.5479577083057389No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19710.10969171674493308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG750.074.6666642
CGTTTTT56700.071.534391
CGAGGGA12750.069.3333364
GGCGCGA1350.068.148158
AGGGATT44200.067.96386
CGTTAGG2200.067.272732
ACGGGAC7600.066.315795
ACGGGAT11150.066.0089655
GCGCGAC1400.065.714299
CGGGACC5700.065.263166
CTACGGG3800.065.263153
CGTAAGG2950.065.084752
GTTAGCG800.065.01
AGTACGG3050.064.262292
TAGGGAC20800.064.230775
ATAGCGG4250.063.0588232
TGTAGGG27150.063.057093
GTAGGGA27150.062.61514
CGGGATT11650.062.4892656
TAAGGGA23300.062.3175934
Produced by FastQC (version 0.11.5)