##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547750_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1796854
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83943993223712	31.0	31.0	33.0	30.0	34.0
2	32.30490011987619	33.0	31.0	34.0	31.0	34.0
3	32.458652177639365	34.0	31.0	34.0	31.0	34.0
4	36.02529921740999	37.0	35.0	37.0	35.0	37.0
5	35.98983278552403	37.0	35.0	37.0	35.0	37.0
6	36.042955075927146	37.0	35.0	37.0	35.0	37.0
7	36.28385277824464	37.0	35.0	37.0	35.0	37.0
8	36.24845312974788	37.0	37.0	37.0	35.0	37.0
9	38.150010518383795	39.0	39.0	39.0	37.0	39.0
10-11	37.59349201437624	39.0	37.5	39.0	35.0	39.0
12-13	36.77980709618032	39.0	35.0	39.0	33.0	39.0
14-15	37.85415064329099	40.0	35.0	41.0	33.0	41.0
16-17	38.03373228987998	40.0	35.0	41.0	34.0	41.0
18-19	37.92802141965903	40.0	36.0	41.0	34.0	41.0
20-21	37.74874975930153	40.0	35.0	41.0	33.5	41.0
22-23	37.563772571394225	39.0	35.0	41.0	33.0	41.0
24-25	37.504860161148315	39.0	35.0	41.0	33.0	41.0
26-27	37.48294547024967	39.0	35.0	41.0	33.0	41.0
28-29	37.48564991924775	39.0	35.0	41.0	33.0	41.0
30-31	37.332993943859655	39.0	35.0	41.0	33.0	41.0
32-33	37.04104785363752	39.0	35.0	41.0	32.5	41.0
34-35	36.782231611472056	39.0	35.0	41.0	31.5	41.0
36-37	36.57959522587812	39.0	35.0	41.0	31.0	41.0
38-39	36.50349972785769	39.0	35.0	41.0	31.0	41.0
40-41	36.371582499190254	39.0	35.0	41.0	30.5	41.0
42-43	36.338665523186634	39.0	35.0	41.0	31.0	41.0
44-45	36.224191002719195	38.0	35.0	41.0	30.0	41.0
46-47	36.159065232901504	38.0	35.0	41.0	30.0	41.0
48-49	36.06001099699809	38.0	35.0	40.5	30.0	41.0
50-51	35.8848273148514	38.0	35.0	40.0	30.0	41.0
52-53	35.71561796339603	37.5	35.0	40.0	29.0	41.0
54-55	35.543756754861555	37.0	35.0	40.0	29.0	41.0
56-57	35.26074878649017	37.0	34.5	40.0	28.5	41.0
58-59	34.97437437877535	36.0	34.0	40.0	28.0	41.0
60-61	34.69590907218951	36.0	34.0	39.5	27.5	41.0
62-63	34.336825084286204	35.5	33.5	39.0	26.5	41.0
64-65	34.0761286114509	35.0	34.0	39.0	26.0	40.0
66-67	33.814281516472676	35.0	34.0	38.0	26.0	40.0
68-69	33.455687273423436	35.0	33.5	37.5	25.5	40.0
70-71	33.220618926189886	35.0	33.0	37.0	25.0	39.0
72-73	32.95286317085306	35.0	33.0	36.5	25.0	39.0
74-75	32.675562677880336	35.0	33.0	36.0	25.0	38.5
76-77	30.61436905836534	33.0	30.0	34.5	21.0	36.0
78-79	31.717141459461928	34.5	31.5	35.0	23.0	37.0
80-81	31.97914966936657	35.0	32.5	35.0	24.0	37.0
82-83	31.859652481503783	35.0	32.5	35.0	23.5	36.0
84-85	31.679121119467695	35.0	32.0	35.0	23.0	36.0
86	31.236778280260946	34.0	31.0	35.0	20.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	13.0
11	9.0
12	26.0
13	70.0
14	188.0
15	643.0
16	1541.0
17	3399.0
18	7113.0
19	11333.0
20	13470.0
21	14088.0
22	12483.0
23	11298.0
24	11590.0
25	13001.0
26	15267.0
27	18604.0
28	23165.0
29	28819.0
30	36555.0
31	45603.0
32	59310.0
33	81729.0
34	205148.0
35	187033.0
36	151585.0
37	269468.0
38	378447.0
39	195076.0
40	769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.650681691445158	23.233273265384945	31.97789024595209	14.138154797217803
2	29.698628825714273	25.90143662200713	31.097239953830417	13.302694598448175
3	28.934849464675484	24.81731960415259	32.42417024421572	13.823660686956202
4	26.17335632165997	27.19725698359466	32.038329213169234	14.591057481576133
5	24.41250096001122	32.10817350769734	29.393039167344703	14.086286364946735
6	22.840086061527536	40.532452831448744	26.372092557325193	10.25536854969853
7	89.87836518715488	3.098804911250441	5.60891424678911	1.4139156548055658
8	91.008618396375	2.5236329718496884	5.237375991594197	1.2303726401811166
9	87.02203963148925	5.0201630182530135	6.316094685489195	1.6417026647685344
10-11	40.2095829711262	28.817505484585837	19.745928161108246	11.226983383179713
12-13	29.750608563633996	25.81670519697204	27.803594504617514	16.62909173477645
14-15	15.921271288596625	32.78135007073474	34.80285543511047	16.49452320555816
16-17	21.37847593627529	25.91365241694651	33.00947099764366	19.69840064913454
18-19	23.360523448204475	27.997015895559684	30.964007092395935	17.67845356383991
20-21	25.998829064576196	27.882398903861972	30.1499732309915	15.96879880057033
22-23	21.2662241896114	23.97974459805861	32.42361371597247	22.330417496357523
24-25	18.92549422490642	27.13261622814096	35.21952813083311	18.722361416119508
26-27	17.91300239195839	33.916862471853584	29.608832993665597	18.561302142522432
28-29	16.460686288368446	28.650908754968405	36.33122112314078	18.557183833522366
30-31	21.925710157864803	27.994901088235324	31.821644941659144	18.25774381224073
32-33	24.58661082091255	28.30744734964555	29.442458875345466	17.663482954096438
34-35	19.652487069066268	28.52518902481782	30.5288854854095	21.293438420706412
36-37	22.380143294892072	28.522851606196163	30.973328940470402	18.123676158441366
38-39	19.603679542133083	30.326503989750975	28.979009980777516	21.090806487338426
40-41	21.062813116702863	26.45587788434675	29.62188358096985	22.859425417980535
42-43	21.393975247849852	26.460664027238717	29.224633720936705	22.920727003974726
44-45	19.091172682922487	31.439504823430287	29.245058307464046	20.224264186183184
46-47	21.26797725357764	30.072281888233547	28.50287224226342	20.15686861592539
48-49	21.568307720048484	27.311901801704536	31.178520903757345	19.941269574489638
50-51	19.505647092084278	31.19338020785217	28.937019924824163	20.36395277523939
52-53	21.590179280008282	27.21103105761514	30.80495132047456	20.39383834190201
54-55	22.004709341994396	27.54255493212025	31.03816448080924	19.414571245076118
56-57	22.864016775987363	25.284330279477352	30.31581864748054	21.53583429705474
58-59	23.095504698767957	27.166675756627974	29.034022797622956	20.703796746981112
60-61	23.682753301047274	29.06735883939374	27.910197489612397	19.339690369946585
62-63	22.644494210436687	26.71641658142509	30.88781837589476	19.751270832243463
64-65	20.065876248153717	30.19800161838413	30.971687182152806	18.764434951309344
66-67	18.542018438893756	29.35077084726973	27.03310897824754	25.07410173558898
68-69	18.655744985402265	31.2873778281374	26.0186136436238	24.038263542836535
70-71	19.0951796862739	29.903319913582294	29.333796735850548	21.66770366429326
72-73	22.15797722018595	29.689028713518184	27.860972566496777	20.29202149979909
74-75	21.501051281851502	31.468889514673982	25.77137597155918	21.258683231915338
76-77	25.388623672262746	28.093573545763874	26.90652662931991	19.61127615265347
78-79	19.266228641837344	28.996373661966974	30.51074266467949	21.226655031516195
80-81	16.97516882284259	28.64164255971826	31.350376825273507	23.032811792165642
82-83	18.859935197851353	27.305557379731464	31.89176750030887	21.942739922108306
84-85	17.656275913346324	26.928676453401334	30.687802125270053	24.727245507982285
86	18.42331096460814	27.041707339605775	31.089726822546517	23.445254873239563
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	786.0
1	946.0
2	3722.0
3	4031.0
4	1851.0
5	2255.0
6	2898.5
7	3265.0
8	3379.5
9	3511.0
10	3569.5
11	3552.0
12	3492.5
13	3562.5
14	3633.0
15	3647.5
16	3717.5
17	3929.5
18	4142.5
19	4643.5
20	5205.5
21	5323.0
22	5630.0
23	6498.5
24	8329.5
25	11214.0
26	13753.0
27	16706.0
28	20438.5
29	22142.0
30	23333.0
31	28117.0
32	34317.0
33	39072.0
34	45322.5
35	50290.5
36	51156.0
37	53427.5
38	58916.0
39	64121.0
40	71956.5
41	81744.5
42	91233.5
43	96782.0
44	96656.0
45	97172.5
46	100772.0
47	103972.0
48	102218.0
49	88124.0
50	76027.0
51	69686.0
52	56869.5
53	49638.0
54	45342.0
55	40722.5
56	37799.5
57	35956.0
58	31380.5
59	23098.0
60	17118.5
61	13431.0
62	11065.5
63	8058.5
64	6002.0
65	5144.5
66	3723.0
67	2796.5
68	1920.5
69	1155.5
70	713.0
71	522.0
72	467.0
73	398.0
74	272.0
75	105.0
76	45.5
77	34.0
78	19.0
79	11.0
80	14.5
81	17.5
82	12.5
83	8.0
84	4.5
85	0.5
86	0.0
87	1.5
88	2.5
89	1.5
90	0.5
91	0.5
92	0.5
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1796854.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.773093188403756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.439474770566	12.6877933158239
2	7.78230932403247	2.4550218037789517
3	2.3974997705877614	1.1344796190197217
4	1.151989903802052	0.7268177641908017
5	0.6911572016565815	0.5450843474782813
6	0.4767385276555145	0.45117847339276984
7	0.38249363186173335	0.42231753895282836
8	0.28964554351666516	0.3654884919595372
9	0.2202342636327601	0.3126398007203105
>10	2.9244055275577265	11.391019268688119
>50	1.3043753592032339	15.069176182451322
>100	1.93398797599823	53.24889693606667
>500	0.004621662442642826	0.4646400785304995
>1k	7.110249911758194E-4	0.17332880554402294
>5k	3.555124955879097E-4	0.5521175734022807
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9846	0.5479577083057389	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1971	0.10969171674493308	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022038518432771943	0.0
2	0.0	0.0	0.0	0.07067908689298073	0.0
3	0.0	0.0	0.0	0.1054064492718941	0.0
4	0.0	0.0	0.0	0.17536204944864747	0.0
5	0.0	0.0	0.0	0.3313012632078065	0.0
6	0.0	0.0	0.0	0.4532366013042796	0.0
7	0.0	0.0	0.0	0.5408341467921155	0.0
8	0.0	0.0	0.0	0.7294972212544815	0.0
9	0.0	0.0	0.0	0.788489215039174	0.0
10	0.0	0.0	0.0	0.8974017922435545	0.0
11	0.0	0.0	0.0	1.1048198685035067	0.0
12	0.0	0.0	0.0	1.2672148098843867	0.0
13	0.0	0.0	0.0	1.3181927969662532	0.0
14	0.0	0.0	0.0	1.3367251874665387	0.0
15	0.0	0.0	0.0	1.3666107541291612	0.0
16	0.0	0.0	0.0	1.4396829124681247	0.0
17	0.0	0.0	0.0	1.5459798069292219	0.0
18	0.0	0.0	0.0	1.6567289273363335	0.0
19	0.0	0.0	0.0	1.740597733594382	0.0
20	0.0	0.0	0.0	1.8327031578525579	0.0
21	0.0	0.0	0.0	1.944843598867799	0.0
22	0.0	0.0	0.0	2.0744033738968217	0.0
23	0.0	0.0	0.0	2.206244914723177	0.0
24	0.0	0.0	0.0	2.300799063251661	0.0
25	0.0	0.0	0.0	2.3876731220232696	0.0
26	0.0	0.0	0.0	2.4700949548488635	0.0
27	0.0	0.0	0.0	2.5649830203232984	0.0
28	0.0	0.0	0.0	2.658591070838254	0.0
29	0.0	0.0	0.0	2.7719002211643238	0.0
30	0.0	0.0	0.0	2.9044095958825813	0.0
31	0.0	0.0	0.0	3.022783153222243	0.0
32	0.0	0.0	0.0	3.135257511183435	0.0
33	0.0	0.0	0.0	3.2416100584688574	0.0
34	0.0	0.0	0.0	3.3602618799301447	0.0
35	0.0	0.0	0.0	3.506907072026998	0.0
36	0.0	0.0	0.0	3.629732855312674	0.0
37	0.0	0.0	0.0	3.778158937787934	0.0
38	0.0	0.0	0.0	3.927976340871323	0.0
39	0.0	0.0	0.0	4.13533876430695	0.0
40	0.0	0.0	0.0	4.283430929836258	0.0
41	0.0	0.0	0.0	4.4362535854332075	0.0
42	0.0	0.0	0.0	4.594752829111324	0.0
43	5.565282432518168E-5	0.0	0.0	4.745571983032567	0.0
44	5.565282432518168E-5	0.0	0.0	4.89661374825111	0.0
45	5.565282432518168E-5	0.0	0.0	5.050048584915636	0.0
46	5.565282432518168E-5	0.0	0.0	5.2192888236885135	0.0
47	5.565282432518168E-5	0.0	0.0	5.395485665502038	0.0
48	5.565282432518168E-5	0.0	0.0	5.559995414207275	0.0
49	5.565282432518168E-5	0.0	0.0	5.737694882277581	0.0
50	5.565282432518168E-5	0.0	0.0	5.920403104537153	0.0
51	5.565282432518168E-5	0.0	0.0	6.1100679298373715	0.0
52	5.565282432518168E-5	0.0	0.0	6.321047786854135	0.0
53	5.565282432518168E-5	0.0	0.0	6.5068169144515915	0.0
54	5.565282432518168E-5	0.0	0.0	6.700433090278898	0.0
55	5.565282432518168E-5	0.0	0.0	6.896664948849489	0.0
56	5.565282432518168E-5	0.0	0.0	7.098629048325574	0.0
57	5.565282432518168E-5	0.0	0.0	7.295417435139416	0.0
58	1.1130564865036335E-4	0.0	0.0	7.496157172480347	0.0
59	1.1130564865036335E-4	0.0	0.0	7.699902162334836	0.0
60	1.1130564865036335E-4	0.0	0.0	7.909435045919145	0.0
61	1.1130564865036335E-4	0.0	0.0	8.125813226895453	0.0
62	1.1130564865036335E-4	0.0	0.0	8.373301336669535	0.0
63	1.1130564865036335E-4	0.0	0.0	8.635593097714116	0.0
64	1.1130564865036335E-4	0.0	0.0	8.884082958326053	0.0
65	1.6695847297554502E-4	0.0	0.0	9.107584700815982	0.0
66	1.6695847297554502E-4	0.0	0.0	9.319955878440876	0.0
67	1.6695847297554502E-4	0.0	0.0	9.546017650849763	0.0
68	1.6695847297554502E-4	0.0	0.0	9.809700732502474	0.0
69	1.6695847297554502E-4	0.0	0.0	10.144619429291417	0.0
70	2.226112973007267E-4	0.0	0.0	10.385540505795129	0.0
71	2.226112973007267E-4	0.0	0.0	10.633473838163813	0.0
72	3.3391694595109005E-4	0.0	0.0	10.871000092383689	0.0
73	3.3391694595109005E-4	0.0	0.0	11.12266216398216	0.0
74	3.8956977027627173E-4	0.0	0.0	11.399423659351289	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	75	0.0	74.666664	2
CGTTTTT	5670	0.0	71.53439	1
CGAGGGA	1275	0.0	69.333336	4
GGCGCGA	135	0.0	68.14815	8
AGGGATT	4420	0.0	67.9638	6
CGTTAGG	220	0.0	67.27273	2
ACGGGAC	760	0.0	66.31579	5
ACGGGAT	1115	0.0	66.008965	5
GCGCGAC	140	0.0	65.71429	9
CGGGACC	570	0.0	65.26316	6
CTACGGG	380	0.0	65.26315	3
CGTAAGG	295	0.0	65.08475	2
GTTAGCG	80	0.0	65.0	1
AGTACGG	305	0.0	64.26229	2
TAGGGAC	2080	0.0	64.23077	5
ATAGCGG	425	0.0	63.058823	2
TGTAGGG	2715	0.0	63.05709	3
GTAGGGA	2715	0.0	62.6151	4
CGGGATT	1165	0.0	62.489265	6
TAAGGGA	2330	0.0	62.317593	4
>>END_MODULE