FastQCFastQC Report
Thu 26 May 2016
SRR1547749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547749_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1785216
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132480.7420950742095075No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33240.1861959561195956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2150.076.279075
GGCGATA2000.076.08
ATCGTAG850.075.294111
ACGGGAT8150.075.0920265
CGAAACG4700.073.1914980
CCGATCC11000.069.81818480
TACGGGA10550.069.383894
CGTTTTT61350.069.111651
CGTAAGG3800.068.421052
CGAAGGG12000.068.3333363
ATTAGCG2050.068.292691
TTGCACG1550.067.096771
CGAACGG1200.066.666672
GTCGACG303.7964437E-666.666671
GTTACGG2100.066.666672
CTACGCG303.7964437E-666.666671
CGGGACC5550.066.306316
GCACGGG6050.065.4545443
CGAGGGA11000.065.4545444
CGGGATA5400.065.185186
Produced by FastQC (version 0.11.5)