FastQCFastQC Report
Thu 26 May 2016
SRR1547748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547748_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences155748
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.28443382900583No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGG150.001307653480.02
CGAAACG150.001307653480.080
GTTAGCG150.001307653480.01
GCGTTAG204.079788E-580.01
GCACCGA204.079788E-580.09
ATGCGCG204.079788E-580.01
ATTGCGG451.8189894E-1280.02
CGTTTTT1850.071.351351
TGACGGG1350.071.111113
GAGGGAT3100.070.9677355
TACGGGG800.070.04
CGATGGG403.6852725E-970.03
GCGCAGG351.1747943E-768.571432
TGGGACC1050.068.571436
TGAACGG303.780202E-666.6666642
TCGATGG303.780202E-666.6666642
CGTTAGG303.780202E-666.6666642
GTGACGG303.780202E-666.6666642
AGGGATT3400.065.8823556
AGGGATG2800.065.714296
Produced by FastQC (version 0.11.5)