FastQCFastQC Report
Thu 26 May 2016
SRR1547747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547747_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences580140
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29750.512807253421588No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20040.34543386079222255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8190.14117282035370773No Hit
GAGCTGGGCTGGCAAGGAAAGGTTGGGGTGTGCTGGCTGAAGGAGAGAAA6190.10669838314889508No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG204.0891908E-580.02
TCGTTAG150.001309462280.01
TTAGCGA204.0891908E-580.01
GGGTACG451.8189894E-1280.07
TTCGTAG251.284001E-680.01
ACGTGCG304.0565283E-880.01
TAGGGTC950.080.05
ACTAACG204.0891908E-580.01
CTCGAGG251.284001E-680.02
GGCGATT404.1836756E-1180.08
GCTAATG600.080.01
GACTACG304.0565283E-880.01
AGTACGG1500.074.666672
CGTTAGG700.074.285712
CTAGACG650.073.846151
GCGTAAG600.073.333331
GCGTTAG550.072.727271
CGTTTGG550.072.727272
AGGCGAG1050.072.380961
GGCGATA1050.072.380968
Produced by FastQC (version 0.11.5)