##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547747_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	580140
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.774831937118627	31.0	31.0	33.0	30.0	34.0
2	32.21421898162512	33.0	31.0	34.0	31.0	34.0
3	32.38768228358672	34.0	31.0	34.0	31.0	34.0
4	35.976828007032786	37.0	35.0	37.0	35.0	37.0
5	35.90353880097907	37.0	35.0	37.0	35.0	37.0
6	35.964281035612096	37.0	35.0	37.0	35.0	37.0
7	36.201949529423935	37.0	35.0	37.0	35.0	37.0
8	36.15950977350295	37.0	35.0	37.0	35.0	37.0
9	38.083498810631916	39.0	39.0	39.0	37.0	39.0
10-11	37.48305150480918	39.0	37.5	39.0	35.0	39.0
12-13	35.840396283655664	37.0	35.0	39.0	32.5	39.0
14-15	36.64633536732513	38.0	35.0	41.0	32.5	41.0
16-17	37.015881511359325	38.0	35.0	41.0	33.0	41.0
18-19	36.98598355569345	37.0	35.0	41.0	33.0	41.0
20-21	36.690136863515704	36.0	35.0	41.0	33.0	41.0
22-23	36.45492381149378	36.0	35.0	41.0	32.0	41.0
24-25	36.377239976557384	35.0	35.0	40.0	32.0	41.0
26-27	36.331926776295376	35.0	35.0	40.0	32.5	41.0
28-29	36.402128796497394	36.0	35.0	40.0	32.0	41.0
30-31	36.282808460026885	36.0	35.0	40.0	32.0	41.0
32-33	35.87539042300135	35.0	35.0	40.0	31.0	41.0
34-35	35.62795273554659	35.0	35.0	40.0	30.5	41.0
36-37	35.369734029716966	35.0	35.0	40.0	30.0	41.0
38-39	35.367413900093084	35.0	35.0	40.0	30.0	41.0
40-41	35.20470920812218	36.0	35.0	40.0	29.0	41.0
42-43	35.17054159340849	35.0	35.0	40.0	29.5	41.0
44-45	35.0323680490916	35.0	34.0	40.0	29.0	41.0
46-47	34.95650015513497	35.0	34.0	40.0	29.0	41.0
48-49	34.92743648084945	35.5	34.5	40.0	29.0	41.0
50-51	34.752146033716	35.0	34.0	40.0	29.0	41.0
52-53	34.55414382735202	35.0	34.0	39.0	28.5	41.0
54-55	34.36920398524494	35.0	34.0	39.0	28.0	41.0
56-57	34.04188644120384	35.0	34.0	38.5	26.5	41.0
58-59	33.75513582928259	35.0	34.0	38.0	25.5	40.0
60-61	33.49291377943255	35.0	33.0	37.0	25.5	40.0
62-63	32.98684714034543	35.0	32.5	37.0	24.0	40.0
64-65	32.79436084393423	35.0	32.5	36.0	24.5	40.0
66-67	32.67238080463336	35.0	33.0	36.0	24.0	39.0
68-69	32.52844399627676	35.0	33.0	35.5	23.5	39.0
70-71	32.36584100389561	35.0	33.0	35.0	24.0	38.0
72-73	32.1806167476816	35.0	32.5	35.0	23.5	37.5
74-75	31.95614162098804	35.0	32.0	35.0	23.0	37.0
76-77	29.915799634570966	32.5	29.5	34.5	19.0	35.0
78-79	30.795033957320648	34.0	30.5	35.0	19.5	36.0
80-81	31.46289947254111	34.5	32.0	35.0	20.5	36.0
82-83	31.467243251628915	35.0	32.0	35.0	20.0	36.0
84-85	31.351242803461233	35.0	32.0	35.0	20.0	35.0
86	30.9556055434895	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	6.0
12	9.0
13	22.0
14	119.0
15	338.0
16	786.0
17	1635.0
18	2882.0
19	4236.0
20	5130.0
21	5394.0
22	4842.0
23	4457.0
24	4927.0
25	5440.0
26	6376.0
27	7733.0
28	9162.0
29	11520.0
30	14114.0
31	17282.0
32	22444.0
33	32438.0
34	107423.0
35	86000.0
36	39814.0
37	68764.0
38	80007.0
39	36677.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.58709966559796	24.936911779915192	29.79556658737546	14.680421967111387
2	28.89337056572551	26.0766366739063	31.969179853138897	13.060812907229288
3	28.25524873306443	23.924052814837797	33.31006308822008	14.510635363877686
4	26.05129796256076	27.55438342469059	31.506015789292242	14.888302823456407
5	24.4254835039818	31.47223084083152	29.506326059227085	14.595959595959595
6	22.787430620195124	40.94890888406247	25.817733650498155	10.445926845244252
7	88.37470265797911	4.5252869996897305	5.798600337849485	1.301410004481677
8	90.03137173785638	3.050987692625918	5.3666356396731825	1.5510049298445203
9	86.669079877271	4.784707139655945	6.509635605198745	2.0365773778743064
10-11	56.05560726721136	20.602182231875066	15.004567862929639	8.337642637983935
12-13	36.246940393698075	31.20608818561037	20.209863136484294	12.33710828420726
14-15	12.414675767918089	37.34219326369497	37.33771158685835	12.905419381528596
16-17	14.300255110835316	19.881063191643396	39.15451442755197	26.66416726996932
18-19	23.976884889854173	24.60001034233116	31.2507325817906	20.172372186024063
20-21	28.701606508773743	25.829110214775742	27.031492398386597	18.437790878063915
22-23	19.850467128624125	25.867980142724168	26.301151446202642	27.980401282449062
24-25	16.979349812114318	23.878201813355396	40.94908125624849	18.193367118281795
26-27	14.450391284862274	40.03688764780915	28.007636087840865	17.50508497948771
28-29	12.380718447271349	28.252835522460096	42.1303478470714	17.23609818319716
30-31	22.96583583273003	31.28710311304168	29.63991450339573	16.107146550832557
32-33	30.78817182059503	28.441841624435483	25.443168890267863	15.326817664701622
34-35	18.87078980935636	29.165804805736546	28.50889440479884	23.45451098010825
36-37	25.283207501637534	28.521563760471608	30.110662943427446	16.084565794463405
38-39	17.943255076360877	33.89406005446961	27.216016823525358	20.946668045644152
40-41	20.078515530733963	26.13360568138725	27.31297617816389	26.474902609714896
42-43	22.147240321301755	23.064001792670734	26.427672630744304	28.361085255283207
44-45	16.2213431240735	36.59702830351294	26.37544385837901	20.806184714034544
46-47	21.23875271486193	33.65739304305857	24.30689143999724	20.796962802082255
48-49	21.514289654221393	24.17063122694522	33.40555727927742	20.90952183955597
50-51	17.68245595890647	34.94113489847278	26.702692453545694	20.67371668907505
52-53	24.783155789981727	25.34009032302548	29.71627538180439	20.160478505188404
54-55	26.14791257282725	26.64942944806426	29.54424794015238	17.658410038956113
56-57	25.14341365877202	21.74828489674906	28.769004033509155	24.339297410969767
58-59	27.61066294342745	25.241665804805734	26.32562829661806	20.822042955148756
60-61	28.174750922191194	29.36058537594374	24.40109283965939	18.063570862205676
62-63	26.484469266039234	23.08916813183025	31.84843313682904	18.57792946530148
64-65	17.31763022718654	32.05346985210467	32.099320853587066	18.52957906712173
66-67	15.352414934326198	27.81983659116765	23.3416933843555	33.48605509015065
68-69	16.070862205674494	34.56045092563864	19.80573654635088	29.56295032233599
70-71	18.455631399317408	31.103095804460988	27.694091081463096	22.747181714758508
72-73	24.35765504878133	30.328886130933913	24.493139586996243	20.82031923328852
74-75	24.55329747991864	34.25905815837557	19.80237528872341	21.385269072982382
76-77	33.67678491398628	26.66218498983004	21.857999793153375	17.803030303030305
78-79	18.976195401110076	28.711086979005067	32.1798186644603	20.13289895542455
80-81	13.571120763953529	27.413210604336886	34.67059675250802	24.345071879201573
82-83	17.560933567759506	23.192505257351673	35.24554417899128	24.001016995897544
84-85	14.352570069293618	24.059882097424758	31.223756334677837	30.36379149860379
86	17.65660013100286	23.025131864722308	33.91698555521081	25.401282449064016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	543.0
1	514.0
2	1170.5
3	1188.0
4	593.5
5	733.0
6	905.5
7	1012.0
8	1024.0
9	1080.5
10	1078.5
11	1089.5
12	1159.5
13	1206.0
14	1240.0
15	1252.5
16	1326.0
17	1422.0
18	1435.0
19	1433.5
20	1471.5
21	1489.0
22	1566.0
23	1679.0
24	2119.0
25	2508.5
26	2773.5
27	3604.5
28	4859.0
29	5562.0
30	6418.0
31	7637.0
32	8411.5
33	8588.5
34	8949.5
35	9420.0
36	9295.0
37	9917.5
38	10519.0
39	12793.0
40	17999.5
41	26150.5
42	32715.5
43	34041.0
44	36035.0
45	38106.0
46	40744.5
47	44696.5
48	45520.0
49	37645.5
50	30338.0
51	24435.5
52	15963.5
53	13358.0
54	13093.0
55	12443.0
56	12766.5
57	13511.0
58	12398.5
59	9055.0
60	6102.0
61	4668.5
62	3751.0
63	2791.0
64	2037.0
65	1661.0
66	1278.0
67	1075.5
68	912.5
69	731.5
70	638.5
71	759.0
72	515.0
73	270.5
74	211.0
75	133.5
76	73.5
77	28.5
78	16.5
79	8.0
80	10.0
81	8.5
82	4.5
83	3.0
84	1.0
85	1.0
86	2.0
87	1.0
88	0.5
89	1.0
90	1.0
91	0.5
92	0.5
93	1.0
94	1.5
95	2.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	580140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.71648222842762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.23142123552522	13.913365739304307
2	6.429226946039865	2.1494811597200676
3	1.8034832283277822	0.9044368600682594
4	0.8836964703698739	0.5908918536904885
5	0.5485723713381248	0.458510014824008
6	0.37327668876767134	0.37439238804426517
7	0.26088122170779243	0.30527114144861583
8	0.17220222934862187	0.23028924052814836
9	0.18148258901411646	0.27303754266211605
>10	2.469606822095505	10.587789154342056
>50	1.7086173295249487	20.949253628434516
>100	1.9334082636447065	48.15716895921674
>500	0.002062302147887687	0.24787120350260283
>1k	0.002062302147887687	0.8582411142138104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2975	0.512807253421588	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2004	0.34543386079222255	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	819	0.14117282035370773	No Hit
GAGCTGGGCTGGCAAGGAAAGGTTGGGGTGTGCTGGCTGAAGGAGAGAAA	619	0.10669838314889508	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04964318957493019	0.0
2	0.0	0.0	0.0	0.13962147067949116	0.0
3	0.0	0.0	0.0	0.19857275829972076	0.0
4	0.0	0.0	0.0	0.3092357017271693	0.0
5	0.0	0.0	0.0	0.49005412486641153	0.0
6	0.0	0.0	0.0	0.6517392353569827	0.0
7	0.0	0.0	0.0	0.7487847760885303	0.0
8	0.0	0.0	0.0	0.9387389250870479	0.0
9	0.0	0.0	0.0	0.9942427689867963	0.0
10	0.0	0.0	0.0	1.0978384527872582	0.0
11	0.0	0.0	0.0	1.307098286620471	0.0
12	0.0	0.0	0.0	1.4625779984141758	0.0
13	0.0	0.0	0.0	1.522908263522598	0.0
14	0.0	0.0	0.0	1.5453166477057263	0.0
15	0.0	0.0	0.0	1.5827214120729478	0.0
16	0.0	0.0	0.0	1.6656324335505224	0.0
17	0.0	0.0	0.0	1.7583686696314682	0.0
18	0.0	0.0	0.0	1.885924087289275	0.0
19	0.0	0.0	0.0	1.9595270107215499	0.0
20	0.0	0.0	0.0	2.0322680732237046	0.0
21	0.0	0.0	0.0	2.134829523908022	0.0
22	0.0	0.0	0.0	2.2258420381287274	0.0
23	0.0	0.0	0.0	2.3299548384872617	0.0
24	0.0	0.0	0.0	2.4059709725238734	0.0
25	0.0	0.0	0.0	2.4707829144689213	0.0
26	0.0	0.0	0.0	2.5442134657151723	0.0
27	0.0	0.0	0.0	2.6024752645913054	0.0
28	0.0	0.0	0.0	2.674699210535388	0.0
29	0.0	0.0	0.0	2.753818043920433	0.0
30	0.0	0.0	0.0	2.8593098217671598	0.0
31	0.0	0.0	0.0	2.9546316406384667	0.0
32	0.0	0.0	0.0	3.043748060812907	0.0
33	0.0	0.0	0.0	3.12234977763988	0.0
34	0.0	0.0	0.0	3.212155686558417	0.0
35	0.0	0.0	0.0	3.3469507360292345	0.0
36	0.0	0.0	0.0	3.451580652945841	0.0
37	0.0	0.0	0.0	3.550522287723653	0.0
38	0.0	0.0	0.0	3.6399834522701418	0.0
39	0.0	0.0	0.0	3.741510669838315	0.0
40	0.0	0.0	0.0	3.85837901196263	0.0
41	0.0	0.0	0.0	3.9804185196676665	0.0
42	0.0	0.0	0.0	4.097631606164029	0.0
43	0.0	0.0	0.0	4.198986451546179	0.0
44	0.0	0.0	0.0	4.313096838694109	0.0
45	0.0	0.0	0.0	4.450305098769262	0.0
46	0.0	0.0	0.0	4.5973385734477885	0.0
47	0.0	0.0	0.0	4.729720412314269	0.0
48	0.0	0.0	0.0	4.875892026062674	0.0
49	0.0	0.0	0.0	5.0318888544144516	0.0
50	0.0	0.0	0.0	5.1809907953252665	0.0
51	0.0	0.0	0.0	5.333540179956562	0.0
52	0.0	0.0	0.0	5.4874685420760505	0.0
53	0.0	0.0	0.0	5.636915227358913	0.0
54	0.0	0.0	0.0	5.807046574964664	0.0
55	0.0	0.0	0.0	5.971144896059572	0.0
56	0.0	0.0	0.0	6.148515875478333	0.0
57	0.0	0.0	0.0	6.3077877753645675	0.0
58	0.0	0.0	0.0	6.448271106974179	0.0
59	0.0	0.0	0.0	6.602027096907643	0.0
60	0.0	0.0	0.0	6.760437135863757	0.0
61	1.7237218602406316E-4	0.0	0.0	6.94935705174613	0.0
62	1.7237218602406316E-4	0.0	0.0	7.126038542420795	0.0
63	1.7237218602406316E-4	0.0	0.0	7.3730478849932775	0.0
64	1.7237218602406316E-4	0.0	0.0	7.571620643292999	0.0
65	1.7237218602406316E-4	0.0	0.0	7.758989209501155	0.0
66	1.7237218602406316E-4	0.0	0.0	7.929465301478953	1.7237218602406316E-4
67	1.7237218602406316E-4	0.0	0.0	8.101665115316992	1.7237218602406316E-4
68	1.7237218602406316E-4	0.0	0.0	8.308511738545869	1.7237218602406316E-4
69	1.7237218602406316E-4	0.0	0.0	8.51260040679836	1.7237218602406316E-4
70	1.7237218602406316E-4	0.0	0.0	8.686006825938566	1.7237218602406316E-4
71	1.7237218602406316E-4	0.0	0.0	8.872513531216603	1.7237218602406316E-4
72	1.7237218602406316E-4	0.0	0.0	9.059537353052711	1.7237218602406316E-4
73	3.447443720481263E-4	0.0	0.0	9.270865653118213	1.7237218602406316E-4
74	3.447443720481263E-4	0.0	0.0	9.49305340090323	1.7237218602406316E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	20	4.0891908E-5	80.0	2
TCGTTAG	15	0.0013094622	80.0	1
TTAGCGA	20	4.0891908E-5	80.0	1
GGGTACG	45	1.8189894E-12	80.0	7
TTCGTAG	25	1.284001E-6	80.0	1
ACGTGCG	30	4.0565283E-8	80.0	1
TAGGGTC	95	0.0	80.0	5
ACTAACG	20	4.0891908E-5	80.0	1
CTCGAGG	25	1.284001E-6	80.0	2
GGCGATT	40	4.1836756E-11	80.0	8
GCTAATG	60	0.0	80.0	1
GACTACG	30	4.0565283E-8	80.0	1
AGTACGG	150	0.0	74.66667	2
CGTTAGG	70	0.0	74.28571	2
CTAGACG	65	0.0	73.84615	1
GCGTAAG	60	0.0	73.33333	1
GCGTTAG	55	0.0	72.72727	1
CGTTTGG	55	0.0	72.72727	2
AGGCGAG	105	0.0	72.38096	1
GGCGATA	105	0.0	72.38096	8
>>END_MODULE