FastQCFastQC Report
Thu 26 May 2016
SRR1547746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547746_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2442113
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193880.7939026572480471No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT42850.17546280618464422No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34750.14229480781601833No Hit
ATTGAAGGGATATTAAACTGACAGCATTAATGAATTACTTTTTGCTTATT33230.13607068960363422No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT30100.12325391986365906No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT27800.11383584625281468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG1350.080.01
CGTAAGG4100.078.048782
TAGCGGG8000.072.53
CGTTTTT84200.071.068881
TAGTCGA403.7161954E-970.000011
AGACCGG2250.069.3333362
CGAGGGA10400.069.230774
CAGTCGA351.1820521E-768.571437
TAGGGAC21300.067.605635
ATAGCGG3000.066.666672
GTTAGCG303.796864E-666.6666641
GGACGTA850.065.8823558
CGTTAGG3200.065.000012
CGATTGG6200.064.516132
GGGCGAT12800.063.4375047
CGGGCGA3450.062.6086966
TAGGGAT30050.062.5623975
TTAGACG1750.061.7142831
TTACGGG9350.061.6042753
TAGGGTA16900.061.5384645
Produced by FastQC (version 0.11.5)