##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547746_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2442113
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848667936332184	31.0	31.0	33.0	30.0	34.0
2	32.281114346469636	33.0	31.0	34.0	31.0	34.0
3	32.40472820053781	34.0	31.0	34.0	31.0	34.0
4	35.99877810731936	37.0	35.0	37.0	35.0	37.0
5	35.958441317007036	37.0	35.0	37.0	35.0	37.0
6	36.02465569775027	37.0	35.0	37.0	35.0	37.0
7	36.261252448187285	37.0	35.0	37.0	35.0	37.0
8	36.20444917987005	37.0	36.0	37.0	35.0	37.0
9	38.053911100755776	39.0	39.0	39.0	35.0	39.0
10-11	37.6013839244949	39.0	37.5	39.0	35.0	39.0
12-13	36.77116558488489	39.0	35.0	39.0	33.0	39.0
14-15	37.81183159829214	40.0	35.0	41.0	33.0	41.0
16-17	37.9702566998333	40.0	35.0	41.0	33.5	41.0
18-19	37.8320714479633	40.0	36.0	41.0	33.5	41.0
20-21	37.60933666869633	39.5	35.0	41.0	33.5	41.0
22-23	37.437563495219095	39.0	35.0	41.0	33.0	41.0
24-25	37.34644035718249	39.0	35.0	41.0	33.0	41.0
26-27	37.292091725485264	39.0	35.0	41.0	33.0	41.0
28-29	37.27889270480113	39.0	35.0	41.0	33.0	41.0
30-31	37.073012591964414	39.0	35.0	41.0	32.5	41.0
32-33	36.72670674944198	39.0	35.0	41.0	31.5	41.0
34-35	36.39808763967925	39.0	35.0	41.0	31.0	41.0
36-37	36.223761554031285	39.0	35.0	41.0	30.0	41.0
38-39	36.12075833509752	38.0	35.0	41.0	30.0	41.0
40-41	35.96687294977751	38.0	35.0	41.0	30.0	41.0
42-43	35.90161347980212	38.0	35.0	41.0	30.0	41.0
44-45	35.785911012307785	38.0	35.0	41.0	29.5	41.0
46-47	35.707623480158375	38.0	35.0	40.5	29.0	41.0
48-49	35.62776742927129	38.0	35.0	40.0	29.0	41.0
50-51	35.460281117212844	37.0	35.0	40.0	29.0	41.0
52-53	35.27771913093292	37.0	35.0	40.0	28.0	41.0
54-55	35.09090672708429	36.5	34.0	40.0	28.0	41.0
56-57	34.797423378852656	36.0	34.0	40.0	26.5	41.0
58-59	34.475413914098155	36.0	34.0	39.5	26.0	41.0
60-61	34.17166629881582	35.0	34.0	39.0	26.0	41.0
62-63	33.77717329214496	35.0	33.5	39.0	25.0	40.5
64-65	33.48871714781421	35.0	33.0	38.5	25.0	40.0
66-67	33.239612786140526	35.0	33.0	37.5	23.5	40.0
68-69	32.83357485914861	35.0	33.0	37.0	22.5	39.5
70-71	32.57815383645229	35.0	33.0	36.5	22.0	39.0
72-73	32.32034103254026	35.0	33.0	36.0	21.5	39.0
74-75	32.05304381902066	35.0	32.5	36.0	20.0	38.5
76-77	30.005555230245285	33.0	29.5	34.5	18.0	36.0
78-79	31.092843779137166	34.0	31.0	35.0	19.0	37.0
80-81	31.314548712528865	35.0	32.0	35.0	19.0	36.0
82-83	31.14513476649115	35.0	32.0	35.0	18.5	36.0
84-85	30.954313129654523	34.0	31.0	35.0	18.0	36.0
86	30.54220750636846	34.0	31.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	11.0
10	33.0
11	53.0
12	83.0
13	184.0
14	388.0
15	1241.0
16	3087.0
17	7003.0
18	13799.0
19	19773.0
20	22722.0
21	23010.0
22	20146.0
23	18313.0
24	18195.0
25	19838.0
26	23289.0
27	28162.0
28	34788.0
29	43381.0
30	54833.0
31	68127.0
32	85510.0
33	116192.0
34	281640.0
35	261438.0
36	214739.0
37	348207.0
38	481071.0
39	231956.0
40	890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.63873293332455	23.050571369957083	28.01012893342773	14.300566763290643
2	32.316686410497795	24.689316178244002	29.23136644373131	13.762630967526892
3	29.99877565043059	24.393097289109882	32.236878473682424	13.371248586777106
4	27.19284488473711	26.204561377790466	30.98705915737724	15.615534580095188
5	23.27992193645421	31.04979171725469	29.811601674451595	15.85868467183951
6	21.49609784641415	40.38347119891667	27.27801702869605	10.842413925973123
7	85.0996657402831	4.390050747037504	8.094220046328733	2.416063466350656
8	85.35002270574704	3.8978949786516845	8.230208839640099	2.521873475961186
9	80.78876775972283	6.148282245743748	9.70319555237616	3.359754442157263
10-11	39.97497249308283	25.61775396961566	21.303109233684108	13.104164303617399
12-13	29.560384797918854	25.995029714022245	28.271337157617193	16.173248330441712
14-15	16.750555768713404	31.423975876628152	35.70604636231002	16.11942199234843
16-17	21.767195047895	24.45110033810884	34.1082497001572	19.67345491383896
18-19	24.389923807784488	27.840951667674673	30.082453187055634	17.6866713374852
20-21	25.93070017644556	27.72854900653655	29.701451161350846	16.63929965566704
22-23	21.000625278191468	24.729875316989837	32.5785498050254	21.690949599793292
24-25	21.343156520603266	26.59745883994721	33.27917258537995	18.78021205406957
26-27	18.302429084976822	32.99362068831377	29.706590153690676	18.997360073018736
28-29	16.276110073530585	28.312899525943315	36.73011854897788	18.68087185154823
30-31	23.144956846796198	27.650071884470538	31.33759576235825	17.867375506375012
32-33	25.74860377058719	28.281082816397113	28.318509421963682	17.651803991052013
34-35	19.409585060150782	28.068828100910974	31.486319429117327	21.03526740982092
36-37	22.17151704282316	29.46563078776453	29.97828110329047	18.38457106612184
38-39	20.124662536090675	31.324021451914795	27.90982644947224	20.641489562522292
40-41	21.03897321704606	26.585747670152855	29.643202423475078	22.732076689326007
42-43	20.76625856379291	27.058575913563377	28.5420862998559	23.633079222787806
44-45	19.621102709006504	30.287869562137377	29.481068238857087	20.60995948999903
46-47	20.907222556859573	30.200301951629594	28.092967033057032	20.799508458453804
48-49	20.068010775914136	27.821173713091902	31.426207550592462	20.684607960401504
50-51	18.732507463823335	30.482496100712787	30.073792654148274	20.711203781315607
52-53	21.748666011769316	26.731175011148135	30.30564105755958	21.214517919522972
54-55	20.698980759694578	27.48396572967754	31.26163695127949	20.555416559348398
56-57	22.258757068161874	25.4114776834651	30.00284999097093	22.326915257402096
58-59	23.17022185296094	27.535928927121716	28.683234559580168	20.610614660337177
60-61	23.88394804007841	29.152275099473286	27.73800802829353	19.225768832154777
62-63	23.6767708947129	26.210335066395373	30.194528263024683	19.91836577586705
64-65	20.34739997698714	30.2979223320133	30.28590405112294	19.068773639876614
66-67	18.83397696994365	30.09299733468517	26.94912151894691	24.12390417642427
68-69	18.332341705727785	32.00539860358632	26.528420265565106	23.133839425120787
70-71	19.28903371793197	30.15329347986764	29.549779228070115	21.00789357413027
72-73	22.12338249704252	30.52854229104059	27.44434839829279	19.903726813624104
74-75	20.878354113835027	31.680597908450594	25.75284190371207	21.68820607400231
76-77	24.098434429528854	28.96972826400744	26.707158923440478	20.224678383023225
78-79	19.04829956680956	29.937394379375565	29.41196824225578	21.60233781155909
80-81	17.516081360690517	29.3755448662695	29.11200259775039	23.996371175289596
82-83	19.413106600718315	28.977344619188383	29.855539035253486	21.754009744839816
84-85	17.614623074362243	27.96484028380341	28.890002223484334	25.53053441835001
86	18.260539131481632	28.199309368567306	30.729372473755312	22.810779026195757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1658.0
1	2435.0
2	8529.5
3	8811.5
4	3958.0
5	4568.5
6	5497.5
7	5998.0
8	6022.5
9	6172.5
10	6287.0
11	6318.5
12	6445.5
13	6589.0
14	6648.0
15	6704.0
16	7161.0
17	7515.0
18	7591.5
19	8278.5
20	10500.0
21	12158.0
22	11486.0
23	12033.5
24	13932.5
25	16015.0
26	19942.5
27	24179.5
28	27515.0
29	29138.0
30	31892.0
31	37530.0
32	43107.5
33	46596.0
34	49648.5
35	54137.5
36	56369.0
37	63216.5
38	67818.0
39	71542.5
40	83593.5
41	100174.5
42	115152.0
43	119629.0
44	125731.5
45	131465.0
46	132817.5
47	134215.0
48	129254.0
49	120890.5
50	117164.0
51	103038.5
52	83846.0
53	71692.0
54	65794.0
55	63187.5
56	58458.5
57	57525.0
58	49984.5
59	37163.0
60	28373.5
61	23717.0
62	19521.0
63	14727.5
64	12982.0
65	11115.5
66	7417.5
67	5112.0
68	4243.5
69	3175.0
70	2233.5
71	1966.0
72	1570.0
73	1159.5
74	946.0
75	513.0
76	272.0
77	219.5
78	180.5
79	187.0
80	143.5
81	70.0
82	42.5
83	33.0
84	16.0
85	12.0
86	13.0
87	9.5
88	8.0
89	8.5
90	6.0
91	2.5
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2442113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.110132249682273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.05728995176294	11.945661064726156
2	9.7473286037295	2.945668485669272
3	3.0956458244584275	1.4032685341723066
4	1.4795190216095089	0.8942291232976083
5	0.8070582219857313	0.6097378233698918
6	0.5232791341672747	0.47440901524600526
7	0.3900648001268083	0.4125751501103369
8	0.2929857586119487	0.3541642847920025
9	0.23596513467943908	0.32089179521883726
>10	2.083285835413517	6.840249990012705
>50	0.5921247680369597	6.581307999252875
>100	1.6131679830562695	58.16118246601037
>500	0.07654415103415765	6.671347990137083
>1k	0.00546743935958269	1.5844487620083232
>5k	0.0	0.0
>10k+	2.7337196797913446E-4	0.8008575159762598
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19388	0.7939026572480471	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	4285	0.17546280618464422	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3475	0.14229480781601833	No Hit
ATTGAAGGGATATTAAACTGACAGCATTAATGAATTACTTTTTGCTTATT	3323	0.13607068960363422	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3010	0.12325391986365906	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2780	0.11383584625281468	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0948146134106E-5	0.0	0.0	0.05605801205759111	0.0
2	4.0948146134106E-5	0.0	0.0	0.16993480645653988	0.0
3	4.0948146134106E-5	0.0	0.0	0.23463287734842736	0.0
4	4.0948146134106E-5	0.0	0.0	0.34863251618577845	0.0
5	4.0948146134106E-5	0.0	0.0	0.6020196444636263	0.0
6	4.0948146134106E-5	0.0	0.0	0.7922237832565487	0.0
7	4.0948146134106E-5	0.0	0.0	0.9170746808194379	0.0
8	4.0948146134106E-5	0.0	0.0	1.1560480616580806	0.0
9	4.0948146134106E-5	0.0	0.0	1.2432266647775922	0.0
10	4.0948146134106E-5	0.0	0.0	1.4118920787039748	0.0
11	4.0948146134106E-5	0.0	0.0	1.7200678265092566	0.0
12	4.0948146134106E-5	0.0	0.0	1.951629592897626	0.0
13	4.0948146134106E-5	0.0	0.0	2.0404051737163678	0.0
14	4.0948146134106E-5	0.0	0.0	2.078855482936293	0.0
15	4.0948146134106E-5	0.0	0.0	2.146051390742361	0.0
16	4.0948146134106E-5	0.0	0.0	2.2793376064088764	0.0
17	4.0948146134106E-5	0.0	0.0	2.432647465534969	0.0
18	4.0948146134106E-5	0.0	0.0	2.6244076338809874	0.0
19	4.0948146134106E-5	0.0	0.0	2.745532250145673	0.0
20	4.0948146134106E-5	0.0	0.0	2.8847968951477676	0.0
21	4.0948146134106E-5	0.0	0.0	3.0486304278303256	0.0
22	4.0948146134106E-5	0.0	0.0	3.2165178269801604	0.0
23	4.0948146134106E-5	0.0	0.0	3.3803513596627184	0.0
24	8.1896292268212E-5	0.0	0.0	3.50851905706247	0.0
25	8.1896292268212E-5	0.0	0.0	3.618669570163215	0.0
26	8.1896292268212E-5	0.0	0.0	3.7366411791755745	0.0
27	8.1896292268212E-5	0.0	0.0	3.870214031865028	0.0
28	8.1896292268212E-5	0.0	0.0	4.01283642485012	0.0
29	8.1896292268212E-5	0.0	0.0	4.171469542973647	0.0
30	1.22844438402318E-4	0.0	0.0	4.345908645504938	0.0
31	1.22844438402318E-4	0.0	0.0	4.511953378078737	0.0
32	1.22844438402318E-4	0.0	0.0	4.652651208195525	0.0
33	1.22844438402318E-4	0.0	0.0	4.804118400745584	0.0
34	1.22844438402318E-4	0.0	0.0	4.980195429122239	0.0
35	1.22844438402318E-4	0.0	0.0	5.166878027347629	0.0
36	1.22844438402318E-4	0.0	0.0	5.329073634184823	0.0
37	1.22844438402318E-4	0.0	0.0	5.508221773521536	0.0
38	1.22844438402318E-4	0.0	0.0	5.7035853787273565	0.0
39	1.22844438402318E-4	0.0	0.0	6.014095170862282	0.0
40	1.22844438402318E-4	0.0	0.0	6.214659190627133	0.0
41	1.22844438402318E-4	0.0	0.0	6.428408513447167	0.0
42	1.22844438402318E-4	0.0	0.0	6.633763466309708	0.0
43	1.22844438402318E-4	0.0	0.0	6.819749946050817	0.0
44	1.22844438402318E-4	0.0	0.0	7.011059684789361	0.0
45	1.22844438402318E-4	0.0	0.0	7.19225523143278	0.0
46	1.22844438402318E-4	0.0	0.0	7.404243783969046	0.0
47	1.22844438402318E-4	0.0	0.0	7.627656869276729	0.0
48	1.22844438402318E-4	0.0	0.0	7.828712266795189	0.0
49	1.22844438402318E-4	0.0	0.0	8.045491752429147	0.0
50	1.22844438402318E-4	0.0	0.0	8.263213045424187	0.0
51	1.22844438402318E-4	0.0	0.0	8.509352351836299	0.0
52	1.22844438402318E-4	0.0	0.0	8.791648871284826	0.0
53	1.22844438402318E-4	0.0	0.0	9.021122282220356	0.0
54	1.22844438402318E-4	0.0	0.0	9.266483573855918	0.0
55	1.22844438402318E-4	0.0	0.0	9.500461280866201	0.0
56	1.22844438402318E-4	0.0	0.0	9.751514364814405	0.0
57	1.22844438402318E-4	0.0	0.0	9.985246382947881	0.0
58	1.22844438402318E-4	0.0	0.0	10.20583404617231	0.0
59	1.22844438402318E-4	0.0	0.0	10.450294478592923	0.0
60	1.22844438402318E-4	0.0	0.0	10.713058732335481	0.0
61	1.22844438402318E-4	0.0	0.0	10.987370363287857	0.0
62	1.63792584536424E-4	0.0	0.0	11.304800392119448	0.0
63	1.63792584536424E-4	0.0	0.0	11.619404998867784	0.0
64	1.63792584536424E-4	0.0	0.0	11.910792006758083	0.0
65	1.63792584536424E-4	0.0	0.0	12.179616586128487	0.0
66	1.63792584536424E-4	0.0	0.0	12.424240811133638	0.0
67	2.8663702293874196E-4	0.0	0.0	12.69732399770199	0.0
68	2.8663702293874196E-4	0.0	0.0	13.006073019553149	0.0
69	2.8663702293874196E-4	0.0	0.0	13.429845383895012	0.0
70	3.27585169072848E-4	0.0	0.0	13.724753932352844	0.0
71	3.27585169072848E-4	0.0	0.0	13.999352200328158	0.0
72	3.27585169072848E-4	0.0	0.0	14.298765044860742	0.0
73	3.6853331520695395E-4	0.0	0.0	14.591011963819856	0.0
74	3.6853331520695395E-4	0.0	0.0	14.93231476184763	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	135	0.0	80.0	1
CGTAAGG	410	0.0	78.04878	2
TAGCGGG	800	0.0	72.5	3
CGTTTTT	8420	0.0	71.06888	1
TAGTCGA	40	3.7161954E-9	70.00001	1
AGACCGG	225	0.0	69.333336	2
CGAGGGA	1040	0.0	69.23077	4
CAGTCGA	35	1.1820521E-7	68.57143	7
TAGGGAC	2130	0.0	67.60563	5
ATAGCGG	300	0.0	66.66667	2
GTTAGCG	30	3.796864E-6	66.666664	1
GGACGTA	85	0.0	65.882355	8
CGTTAGG	320	0.0	65.00001	2
CGATTGG	620	0.0	64.51613	2
GGGCGAT	1280	0.0	63.437504	7
CGGGCGA	345	0.0	62.608696	6
TAGGGAT	3005	0.0	62.562397	5
TTAGACG	175	0.0	61.714283	1
TTACGGG	935	0.0	61.604275	3
TAGGGTA	1690	0.0	61.538464	5
>>END_MODULE