FastQCFastQC Report
Thu 26 May 2016
SRR1547745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547745_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2932866
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261420.8913465531667658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT151600.073.74671
TCGATAG1800.066.6666641
CGGGTAG4950.064.646466
CGTTAGG3800.064.2105262
GTACGGG11200.063.2142873
TGCGTAG4050.063.2098731
CGAGGGA17850.062.2969174
GCGCGAC2350.061.27669
ATAGGGC10250.061.268294
ATAGCGG8500.061.176472
GGGCGAT19900.060.9045227
CGGGACT10150.060.2955636
CGAGGGT6650.060.1503754
GCGACTA200.004099096660.09
ACGGGTA5000.060.05
TAGCGGG21550.059.9535983
TATAGCG2350.059.574471
AGGGATT54000.059.5555576
TATTGCG4300.059.534881
TAGGGAT44100.059.5011375
Produced by FastQC (version 0.11.5)