##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547745_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2932866
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80471627411549	31.0	31.0	33.0	30.0	34.0
2	32.2657687054233	33.0	31.0	34.0	31.0	34.0
3	32.406574320136	34.0	31.0	34.0	31.0	34.0
4	36.01835883398696	37.0	35.0	37.0	35.0	37.0
5	35.983839698097356	37.0	35.0	37.0	35.0	37.0
6	36.0376900956266	37.0	35.0	37.0	35.0	37.0
7	36.250467290356944	37.0	35.0	37.0	35.0	37.0
8	36.20460123306008	37.0	36.0	37.0	35.0	37.0
9	38.08180053231208	39.0	39.0	39.0	37.0	39.0
10-11	37.5493934942817	39.0	37.0	39.0	35.0	39.0
12-13	36.99251022719756	39.0	35.0	39.0	33.5	39.0
14-15	38.15498099810901	40.0	36.0	41.0	33.0	41.0
16-17	38.26349720716868	40.0	36.0	41.0	34.0	41.0
18-19	38.091340177150954	40.0	36.0	41.0	34.0	41.0
20-21	37.87421893806263	40.0	35.0	41.0	34.0	41.0
22-23	37.677385022022825	39.5	35.0	41.0	33.0	41.0
24-25	37.57483311545771	39.5	35.0	41.0	33.0	41.0
26-27	37.54284904254064	40.0	35.0	41.0	33.0	41.0
28-29	37.50701787943943	40.0	35.0	41.0	33.0	41.0
30-31	37.31467956599449	40.0	35.0	41.0	33.0	41.0
32-33	36.894446592513944	39.5	35.0	41.0	31.5	41.0
34-35	36.521378405968775	39.0	35.0	41.0	30.5	41.0
36-37	36.29759763998764	39.0	35.0	41.0	30.0	41.0
38-39	36.18694324936769	39.0	35.0	41.0	30.0	41.0
40-41	36.02272060844239	39.0	35.0	41.0	29.0	41.0
42-43	35.97490696813288	39.0	35.0	41.0	29.0	41.0
44-45	35.86258884654123	39.0	35.0	41.0	29.0	41.0
46-47	35.77335872146904	38.0	35.0	41.0	29.0	41.0
48-49	35.65410131250456	38.0	35.0	40.5	28.0	41.0
50-51	35.479263116691996	38.0	35.0	40.0	28.0	41.0
52-53	35.28416368153199	38.0	35.0	40.0	27.0	41.0
54-55	35.10869726063175	37.0	34.5	40.0	27.0	41.0
56-57	34.83292059712241	37.0	34.0	40.0	26.0	41.0
58-59	34.5609313552	36.0	34.0	40.0	26.0	41.0
60-61	34.255208898053986	36.0	34.0	39.5	25.5	41.0
62-63	33.91119284004111	35.5	33.5	39.0	25.0	41.0
64-65	33.6381058664119	35.0	33.0	39.0	24.5	40.0
66-67	33.348571158723246	35.0	33.0	38.0	23.5	40.0
68-69	32.927721893874455	35.0	33.0	37.0	22.5	40.0
70-71	32.67032537456536	35.0	33.0	37.0	22.0	39.0
72-73	32.42575317113022	35.0	33.0	36.5	21.5	39.0
74-75	32.120578471706516	35.0	32.5	36.0	20.5	38.5
76-77	30.09328622582825	33.0	29.5	34.5	18.0	36.0
78-79	31.26233401048667	34.0	31.5	35.0	19.0	37.0
80-81	31.44717880053163	35.0	32.0	35.0	19.5	36.5
82-83	31.287284008202217	35.0	32.0	35.0	19.0	36.0
84-85	31.084433281302317	34.0	32.0	35.0	18.5	36.0
86	30.669618386929372	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	13.0
10	12.0
11	32.0
12	48.0
13	173.0
14	552.0
15	1673.0
16	4156.0
17	9692.0
18	20018.0
19	30954.0
20	34071.0
21	30830.0
22	25199.0
23	21458.0
24	20188.0
25	21747.0
26	25425.0
27	30434.0
28	37315.0
29	46177.0
30	57868.0
31	72788.0
32	92660.0
33	125603.0
34	285831.0
35	273097.0
36	268224.0
37	460142.0
38	638430.0
39	297002.0
40	1042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.730759605109814	23.714550886402584	29.821717050830145	15.73297245765746
2	31.88925099203305	25.126889533991665	30.061448426215176	12.92241104776011
3	28.052969348071137	24.029669272309064	34.16705706977407	13.75030430984573
4	25.80782756525528	26.631936133461267	33.09373834331333	14.466497957970123
5	24.261626681887275	30.388807398633283	31.16286253787251	14.186703381606932
6	22.550263121465488	39.55465404829269	27.59161175450907	10.303471075732748
7	86.8218322964636	3.569648255324314	8.11220151210454	1.496317936107548
8	87.50420237406004	3.0863667143333515	7.922557662027518	1.4868732495790806
9	82.95026093929965	5.225025623400455	9.566580948464743	2.258132488835153
10-11	36.04031005848886	26.837571167588294	23.958101052008512	13.16401772191433
12-13	28.55549827370224	24.286499962834988	29.71990537583374	17.438096387629027
14-15	17.68130558982238	30.13577845015763	34.58645911541816	17.596456844601832
16-17	22.144448467812712	26.140011169961397	32.97590138792567	18.739638974300224
18-19	23.459459109280818	27.712960633046315	30.951686848291054	17.875893409381813
20-21	25.97825812703342	26.845975915708387	30.69819759920842	16.477568358049773
22-23	22.24586803488465	24.193451047541892	32.67629683729158	20.884384080281883
24-25	19.972221710777102	27.75788256265373	33.60383665670372	18.666059069865447
26-27	19.293857953278465	32.00918487240808	30.23419753919886	18.462759635114594
28-29	17.453865945460855	27.989413767966216	35.3839725374429	19.172747749130032
30-31	21.902057577809554	28.043115505447574	31.385920802382376	18.668906114360492
32-33	23.503358148650502	28.11134228430484	29.791644077840584	18.593655489204075
34-35	20.227705595823334	28.833417551296243	30.237180969058937	20.701695883821493
36-37	22.462038838460398	29.20515291186164	29.798429249750924	18.534378999927036
38-39	20.720431823342764	29.677932779745138	29.050679437792247	20.55095595911985
40-41	20.69385372533215	27.677006041189745	29.617445870353436	22.011694363124672
42-43	21.201326620445666	27.728252842100527	28.71863221845117	22.35178831900264
44-45	20.184062279013087	30.382857587083762	28.51045700690042	20.922623127002733
46-47	20.909615372812805	29.514457871583634	28.89080169363346	20.6851250619701
48-49	21.35441919269411	27.737032649974463	30.290132587032616	20.618415570298815
50-51	20.009744734331537	30.162714559751453	29.008689793533016	20.818850912383997
52-53	21.855243301262313	27.254756951050613	30.3131646655524	20.576835082134675
54-55	21.214624193536288	27.673937370476526	30.592413700455456	20.51902473553173
56-57	22.013262794822538	27.38127142528844	29.459665051182014	21.145800728707005
58-59	22.545574874542513	28.20517541544687	28.812499445934453	20.43675026407616
60-61	23.066174861040363	29.3992292863022	27.53450038290191	20.00009546975552
62-63	22.63633933497132	27.028578871315634	30.082707494989542	20.252374298723502
64-65	20.542653500023526	30.145086751321067	29.255018810951473	20.057240937703938
66-67	19.574504938173103	29.60128420459714	27.063425332081316	23.76078552514844
68-69	19.59458768317407	31.025454282602748	26.94611686998315	22.43384116424003
70-71	19.92627689093194	29.54325223177602	29.383800691882954	21.146670185409082
72-73	22.100498283931145	29.730338856258694	27.760149969347392	20.409012890462776
74-75	21.12500196053962	30.87713519813043	26.082320160552854	21.915542680777097
76-77	23.539176355142036	28.540836846961298	27.435365270694263	20.4846215272024
78-79	19.99774282220872	29.65906045485883	29.237237568985424	21.105959153947026
80-81	18.773479592998793	28.866218231586444	29.589878978446336	22.770423196968427
82-83	20.304661038042653	28.284227782653552	29.815341035014896	21.5957701442889
84-85	19.06871981195186	28.137886285974194	28.821176964784616	23.97221693728933
86	19.717402704385403	28.192457480157636	29.74708015981637	22.343059655640594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1351.0
1	1781.0
2	8401.0
3	9266.5
4	4293.5
5	5152.5
6	6055.5
7	6451.0
8	6818.0
9	7408.5
10	7657.5
11	7684.0
12	7807.5
13	7864.5
14	7799.0
15	8165.5
16	8894.5
17	9425.0
18	10062.5
19	10381.5
20	10428.0
21	10625.0
22	11604.0
23	13859.5
24	15935.5
25	19090.0
26	23406.0
27	26951.0
28	32190.5
29	35846.0
30	39547.5
31	44808.0
32	53216.0
33	59396.0
34	63580.5
35	75721.5
36	83009.0
37	83993.5
38	86178.0
39	94965.0
40	107803.5
41	118591.5
42	132047.0
43	139966.0
44	144262.5
45	152564.5
46	157493.5
47	154850.0
48	151651.5
49	143693.5
50	135367.0
51	121511.5
52	100493.0
53	88892.5
54	81186.0
55	75956.0
56	67393.5
57	60792.0
58	55879.0
59	43842.5
60	33709.5
61	28744.5
62	24358.0
63	19474.0
64	17021.0
65	13864.0
66	9086.5
67	6775.5
68	5330.0
69	3602.5
70	2182.5
71	1735.0
72	1557.0
73	1081.0
74	739.5
75	581.0
76	376.5
77	219.5
78	144.0
79	136.0
80	93.0
81	46.0
82	35.0
83	24.5
84	19.5
85	15.0
86	12.0
87	12.5
88	9.0
89	4.0
90	2.5
91	1.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2932866.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.235030895412123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.55590897964447	13.883835800721792
2	7.227387536796728	2.4912849497961624
3	2.2297181155620476	1.1528778182931596
4	1.1535173455022167	0.7952362835249794
5	0.7545188987436653	0.6502078265509702
6	0.5375347465760819	0.5558656778757779
7	0.4475783948482874	0.5399819224330439
8	0.31877425514701757	0.4395267308896668
9	0.2803433702132956	0.4348553982255097
>10	3.523465976958134	14.682418172759066
>50	1.3898120056503902	17.473022942247002
>100	1.5764396153371534	45.2426592871063
>500	0.0030004554123375486	0.3383750153993547
>1k	0.0018002732474025293	0.4185990256728441
>5k	0.0	0.0
>10k+	2.0003036082250327E-4	0.901253148504323
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26142	0.8913465531667658	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0228902377401491E-4	0.0	0.0	0.019469010858320836	0.0
2	1.0228902377401491E-4	0.0	0.0	0.057963780138608444	0.0
3	1.0228902377401491E-4	0.0	0.0	0.08892325800087696	0.0
4	1.0228902377401491E-4	0.0	0.0	0.13972680647530436	0.0
5	1.0228902377401491E-4	0.0	0.0	0.2633260435355724	0.0
6	1.0228902377401491E-4	0.0	0.0	0.3958244256641797	0.0
7	1.0228902377401491E-4	0.0	0.0	0.47932636540503387	0.0
8	1.0228902377401491E-4	0.0	0.0	0.6356580900729867	0.0
9	1.0228902377401491E-4	0.0	0.0	0.6961109031234295	0.0
10	1.0228902377401491E-4	0.0	0.0	0.8020482354120508	0.0
11	1.0228902377401491E-4	0.0	0.0	0.9778489709383245	0.0
12	1.0228902377401491E-4	0.0	0.0	1.104891938465651	0.0
13	1.0228902377401491E-4	0.0	0.0	1.1519107930604398	0.0
14	1.0228902377401491E-4	0.0	0.0	1.173800644148079	0.0
15	1.0228902377401491E-4	0.0	0.0	1.2048283146928636	0.0
16	1.0228902377401491E-4	0.0	0.0	1.2729187081850994	0.0
17	1.0228902377401491E-4	0.0	0.0	1.358568717425208	0.0
18	1.0228902377401491E-4	0.0	0.0	1.4514471510120135	0.0
19	1.0228902377401491E-4	0.0	0.0	1.523151756677598	0.0
20	1.0228902377401491E-4	0.0	0.0	1.6010278001108813	0.0
21	1.0228902377401491E-4	0.0	0.0	1.702464415353446	0.0
22	1.0228902377401491E-4	0.0	0.0	1.8157665573537967	0.0
23	1.0228902377401491E-4	0.0	0.0	1.9337058017652358	0.0
24	1.0228902377401491E-4	0.0	0.0	2.0232427939087567	0.0
25	1.0228902377401491E-4	0.0	0.0	2.105210398293001	0.0
26	1.0228902377401491E-4	0.0	0.0	2.1810747575920617	0.0
27	1.0228902377401491E-4	0.0	0.0	2.265599587570656	0.0
28	1.0228902377401491E-4	0.0	0.0	2.353738629722599	0.0
29	1.0228902377401491E-4	0.0	0.0	2.458346204702158	0.0
30	1.0228902377401491E-4	0.0	0.0	2.5703185893934464	0.0
31	1.0228902377401491E-4	0.0	0.0	2.683382057004991	0.0
32	1.0228902377401491E-4	0.0	0.0	2.780147473495209	0.0
33	1.0228902377401491E-4	0.0	0.0	2.8861870948076045	0.0
34	1.0228902377401491E-4	0.0	0.0	2.9979208051100867	0.0
35	1.0228902377401491E-4	0.0	0.0	3.1245546165423175	0.0
36	1.0228902377401491E-4	0.0	0.0	3.241982415834886	0.0
37	1.0228902377401491E-4	0.0	0.0	3.3705256223775653	0.0
38	1.0228902377401491E-4	0.0	0.0	3.5086839971550012	0.0
39	1.0228902377401491E-4	0.0	0.0	3.691474482639166	0.0
40	1.0228902377401491E-4	0.0	0.0	3.840986939055518	0.0
41	1.3638536503201987E-4	0.0	0.0	3.9889309637740014	0.0
42	1.3638536503201987E-4	0.0	0.0	4.141989439681185	0.0
43	1.7048170629002483E-4	0.0	0.0	4.283659737608196	0.0
44	1.7048170629002483E-4	0.0	0.0	4.4312968952553575	0.0
45	1.7048170629002483E-4	0.0	0.0	4.5763086346256525	0.0
46	1.7048170629002483E-4	0.0	0.0	4.737857099506081	0.0
47	1.7048170629002483E-4	0.0	0.0	4.904383630210177	0.0
48	1.7048170629002483E-4	0.0	0.0	5.062658846329835	0.0
49	1.7048170629002483E-4	0.0	0.0	5.225639357543099	0.0
50	1.7048170629002483E-4	0.0	0.0	5.396462027245704	0.0
51	1.7048170629002483E-4	0.0	0.0	5.569466862788822	0.0
52	1.7048170629002483E-4	0.0	0.0	5.760304084809875	0.0
53	2.0457804754802982E-4	0.0	0.0	5.937161806915147	0.0
54	2.0457804754802982E-4	0.0	0.0	6.123771082620208	0.0
55	2.0457804754802982E-4	0.0	0.0	6.304652172993925	0.0
56	2.3867438880603478E-4	0.0	0.0	6.497739753538007	0.0
57	2.3867438880603478E-4	0.0	0.0	6.685951557282194	0.0
58	2.3867438880603478E-4	0.0	0.0	6.87385649395506	0.0
59	2.3867438880603478E-4	0.0	0.0	7.071342502521424	0.0
60	2.3867438880603478E-4	0.0	0.0	7.276738862259647	0.0
61	2.3867438880603478E-4	0.0	0.0	7.488238467083051	0.0
62	2.3867438880603478E-4	0.0	0.0	7.723707799810834	0.0
63	2.3867438880603478E-4	0.0	0.0	7.957199544745651	0.0
64	2.3867438880603478E-4	0.0	0.0	8.18298551655616	0.0
65	2.3867438880603478E-4	0.0	0.0	8.392882593340438	0.0
66	2.3867438880603478E-4	0.0	0.0	8.601484009156913	0.0
67	2.3867438880603478E-4	0.0	0.0	8.81891637735921	0.0
68	2.3867438880603478E-4	0.0	0.0	9.052817278389124	0.0
69	2.3867438880603478E-4	0.0	0.0	9.34413641809752	0.0
70	2.3867438880603478E-4	0.0	0.0	9.578173704492466	0.0
71	2.3867438880603478E-4	0.0	0.0	9.825338082271744	0.0
72	2.3867438880603478E-4	0.0	0.0	10.056886335754855	0.0
73	2.3867438880603478E-4	0.0	0.0	10.303402883050232	0.0
74	2.3867438880603478E-4	0.0	0.0	10.566183385125676	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15160	0.0	73.7467	1
TCGATAG	180	0.0	66.666664	1
CGGGTAG	495	0.0	64.64646	6
CGTTAGG	380	0.0	64.210526	2
GTACGGG	1120	0.0	63.214287	3
TGCGTAG	405	0.0	63.209873	1
CGAGGGA	1785	0.0	62.296917	4
GCGCGAC	235	0.0	61.2766	9
ATAGGGC	1025	0.0	61.26829	4
ATAGCGG	850	0.0	61.17647	2
GGGCGAT	1990	0.0	60.904522	7
CGGGACT	1015	0.0	60.295563	6
CGAGGGT	665	0.0	60.150375	4
GCGACTA	20	0.0040990966	60.0	9
ACGGGTA	500	0.0	60.0	5
TAGCGGG	2155	0.0	59.953598	3
TATAGCG	235	0.0	59.57447	1
AGGGATT	5400	0.0	59.555557	6
TATTGCG	430	0.0	59.53488	1
TAGGGAT	4410	0.0	59.501137	5
>>END_MODULE