FastQCFastQC Report
Thu 26 May 2016
SRR1547744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547744_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1783520
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85430.4789965910110343No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33040.18525163721180585No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG150.001309909980.0000180
TAGCGCG204.0915198E-580.01
GCGATAA500.080.09
TATAGCG2150.074.41861
TCGATAG650.073.846151
GTTAGCG1550.072.2580641
TACGTAG1000.068.01
CGTTTTT49150.067.2227861
TAACGCG1550.067.096781
TGTAGCG1550.067.096781
GGATACG1100.065.4545441
GCGTAAG2150.065.116281
GCGAGGG15150.064.95053
TTAAGCG3400.064.7058880
GTACGGG7000.064.571433
AAGTACT66800.064.4311480
ATACCGG750.063.9999962
CGAGGGA7900.063.2911384
CCGCGAG700.062.8571431
TAGTAGG13950.062.79572
Produced by FastQC (version 0.11.5)