##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547744_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1783520
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.837696241141114	31.0	31.0	33.0	30.0	34.0
2	32.284748138512604	33.0	31.0	34.0	31.0	34.0
3	32.46713297299722	34.0	31.0	34.0	31.0	34.0
4	36.0383701892886	37.0	35.0	37.0	35.0	37.0
5	35.99112092939804	37.0	35.0	37.0	35.0	37.0
6	36.04959742531623	37.0	35.0	37.0	35.0	37.0
7	36.23368451152776	37.0	35.0	37.0	35.0	37.0
8	36.18194693639544	37.0	35.0	37.0	35.0	37.0
9	38.1058479860052	39.0	39.0	39.0	37.0	39.0
10-11	37.5860579079573	39.0	37.5	39.0	35.0	39.0
12-13	36.39797367004576	38.0	35.0	39.0	33.0	39.0
14-15	37.29733504530367	39.0	35.0	41.0	33.0	41.0
16-17	37.560523291020004	39.0	35.0	41.0	33.5	41.0
18-19	37.49738606800036	39.0	35.0	41.0	33.5	41.0
20-21	37.26730033865614	39.0	35.0	41.0	33.0	41.0
22-23	37.072340371848924	38.0	35.0	41.0	33.0	41.0
24-25	36.98525359962322	38.0	35.0	41.0	33.0	41.0
26-27	36.96056281959271	38.0	35.0	41.0	33.0	41.0
28-29	36.98793369292186	38.0	35.0	41.0	33.0	41.0
30-31	36.84169563559702	38.0	35.0	41.0	32.5	41.0
32-33	36.50844762043599	38.0	35.0	41.0	32.0	41.0
34-35	36.25512301516103	38.0	35.0	41.0	31.0	41.0
36-37	36.038743608145694	38.0	35.0	41.0	30.5	41.0
38-39	36.00559119045484	38.0	35.0	41.0	31.0	41.0
40-41	35.82821274782452	37.5	35.0	40.0	30.0	41.0
42-43	35.81091156813493	37.0	35.0	40.0	30.0	41.0
44-45	35.70298174396699	37.0	35.0	40.0	30.0	41.0
46-47	35.65202885305463	37.0	35.0	40.0	30.0	41.0
48-49	35.59532189154032	36.5	35.0	40.0	30.0	41.0
50-51	35.397942832152154	36.0	35.0	40.0	30.0	41.0
52-53	35.205006952543286	36.0	34.5	40.0	29.0	41.0
54-55	35.05280765004037	35.5	34.0	40.0	29.0	41.0
56-57	34.76537885754014	35.0	34.0	39.5	28.5	41.0
58-59	34.471228805956756	35.0	34.0	39.0	27.0	41.0
60-61	34.198812460751775	35.0	34.0	39.0	26.5	40.5
62-63	33.77769467121199	35.0	33.5	38.0	26.0	40.0
64-65	33.576002792231094	35.0	33.0	37.5	26.0	40.0
66-67	33.36625773750785	35.0	33.0	37.0	26.0	40.0
68-69	33.07310431057684	35.0	33.0	36.5	25.0	39.0
70-71	32.89704320669239	35.0	33.0	36.0	25.0	39.0
72-73	32.682347829012286	35.0	33.0	36.0	25.0	38.5
74-75	32.42150662734368	35.0	33.0	35.0	25.0	37.5
76-77	30.42135075580874	33.0	30.0	34.5	20.0	35.5
78-79	31.457209899524536	34.0	31.5	35.0	22.5	36.0
80-81	31.869090898896566	35.0	32.5	35.0	24.0	36.0
82-83	31.77526744864089	35.0	32.5	35.0	23.5	36.0
84-85	31.620234704404773	35.0	32.0	35.0	23.0	36.0
86	31.193591325020186	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	3.0
10	3.0
11	18.0
12	39.0
13	84.0
14	262.0
15	784.0
16	2028.0
17	4167.0
18	7799.0
19	11624.0
20	14093.0
21	14635.0
22	12914.0
23	11605.0
24	12091.0
25	13748.0
26	16476.0
27	20142.0
28	24399.0
29	30575.0
30	37872.0
31	48441.0
32	62423.0
33	90314.0
34	261470.0
35	232428.0
36	140815.0
37	247286.0
38	314731.0
39	149724.0
40	522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.11703821656051	22.666804969947073	30.100531533147933	14.115625280344487
2	29.294989683322868	25.91896922938907	30.85639633982237	13.929644747465685
3	29.023560150713195	24.865434646093117	32.404458598726116	13.70654660446757
4	26.048151969139678	27.03406746209743	31.53780165066834	15.379978918094556
5	24.74028886695972	30.773806853862023	29.473681259531716	15.012223019646543
6	22.58079528124159	39.63415044406567	26.498160940163274	11.28689333452947
7	87.96419440208128	4.196588768278461	5.943303130887234	1.8959136987530278
8	88.97792006817978	3.5426572171884816	5.758331838162734	1.7210908764690052
9	85.31600430609132	4.873901049609761	7.3262424867677405	2.483852157531174
10-11	48.21594375168207	23.036579348703686	17.454584193056426	11.292892706557819
12-13	33.99056360455728	27.62430474567148	23.138484569839417	15.246647079931822
14-15	15.18171929667175	32.899715170000896	35.914764062079485	16.00380147124787
16-17	19.922372611464965	22.496635866152328	34.82663496904997	22.754356553332737
18-19	25.83951960168655	26.032116264465778	29.843343500493408	18.285020633354264
20-21	28.45443280703328	27.253717367901675	26.998856194491793	17.292993630573246
22-23	22.200676190903383	24.342536108369963	29.307100565174487	24.149687135552167
24-25	20.029828653449357	24.920522337848748	35.73668924374271	19.31295976495918
26-27	17.004575222032834	35.799290167758144	28.11106687898089	19.085067731228133
28-29	15.777423297748271	28.160884094375167	37.433754597649596	18.627938010226966
30-31	23.62115367363416	28.763204225352112	29.5968926617027	18.018749439311023
32-33	28.868838028169012	27.59094375168207	26.43356957028797	17.10664864986095
34-35	19.786994258544897	29.200401453305822	29.01764488203104	21.994959406118237
36-37	24.385597021620168	29.95735960348076	28.935952498430073	16.721090876469006
38-39	20.416816183726564	31.535530860321163	26.937012200592086	21.110640755360187
40-41	21.728912487664843	26.069402081277477	27.65923566878981	24.542449762267875
42-43	21.803315914595856	24.650746837714184	27.45368709069705	26.092250156992915
44-45	19.871405983672737	32.252904368888494	27.260922221225442	20.614767426213334
46-47	21.563144792320806	31.091100744594957	26.110052031936842	21.235702431147395
48-49	22.275612272360277	25.255365793487037	31.95024446039293	20.518777473759755
50-51	19.36754283663766	32.97596887054813	26.973204673903297	20.683283618910917
52-53	23.184096617924105	25.745043509464434	29.64881246075177	21.422047411859694
54-55	23.801162868933346	27.05952274154481	30.049340629765858	19.08997375975599
56-57	24.066957477348165	24.286831658742262	27.939664259442004	23.706546604467572
58-59	25.690965057863103	26.39894702610568	26.508197272808825	21.40189064322239
60-61	26.302200143536375	29.43510585807841	25.024894590472773	19.237799407912444
62-63	24.447300843276217	24.314053108459674	30.56147393917646	20.677172109087646
64-65	19.590472772943393	30.455195344038756	30.559483493316584	19.394848389701263
66-67	18.81170382165605	28.368226877186686	24.693779716515653	28.126289584641604
68-69	18.235848210280793	32.67457051224545	23.42398739571185	25.66559388176191
70-71	19.53238539517359	29.724309231183277	28.60335179869023	22.1399535749529
72-73	24.06967681887503	29.814355880505968	25.667219879788284	20.448747420830717
74-75	22.88726787476451	32.13002938010227	23.4207353996591	21.561967345474116
76-77	28.98658271283753	26.24722458957567	25.025287072755003	19.740905624831793
78-79	19.506537633443976	28.6727650937472	30.435823539965913	21.38487373284292
80-81	16.365614066564994	27.72329438413923	31.80163384767202	24.109457701623754
82-83	18.737188256930114	25.887458509015875	32.28783529200682	23.087517942047185
84-85	16.52927917825424	25.883141203911364	29.07433053736431	28.513249080470082
86	19.036063514847044	25.08057100565175	32.91765721718848	22.96570826231273
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	708.0
1	1066.0
2	3429.5
3	3461.5
4	1630.5
5	1955.0
6	2344.5
7	2552.0
8	2623.0
9	2696.0
10	2687.0
11	2732.5
12	2837.0
13	2956.5
14	3028.0
15	3194.5
16	3463.0
17	3588.5
18	3912.5
19	4761.5
20	4631.5
21	3953.0
22	4088.5
23	4360.5
24	5121.0
25	7123.5
26	10575.0
27	11863.5
28	13609.0
29	16139.0
30	18860.5
31	24279.0
32	26295.0
33	24889.0
34	29305.0
35	36415.5
36	38385.0
37	41146.5
38	43643.0
39	47269.5
40	59479.5
41	77596.0
42	91187.5
43	94981.0
44	100696.5
45	106920.5
46	110879.5
47	118797.5
48	118803.5
49	99471.0
50	84600.0
51	73731.0
52	57528.0
53	50918.0
54	49326.0
55	48564.0
56	46967.0
57	45816.0
58	41166.5
59	31706.5
60	23048.5
61	18279.0
62	15172.5
63	11388.0
64	9788.0
65	8511.0
66	5835.5
67	3607.0
68	2806.0
69	2411.5
70	1729.5
71	1471.0
72	1221.5
73	938.0
74	613.5
75	313.0
76	228.5
77	192.0
78	145.5
79	61.0
80	52.5
81	39.0
82	26.0
83	12.5
84	10.0
85	11.5
86	10.0
87	6.5
88	2.5
89	2.0
90	2.5
91	2.0
92	1.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1783520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.490993947825956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.389801228603	11.794191170184437
2	8.643520718941012	2.5050641285216493
3	2.487620089102511	1.0814426296702426
4	1.0761805127301134	0.6237970118696123
5	0.6189232867167088	0.4484406800990189
6	0.40541547174925296	0.35249238884840584
7	0.2952387248105753	0.299481180207573
8	0.22694080098476158	0.2630878218867964
9	0.17665085872717018	0.23038618722243132
>10	1.8230023853664816	6.141483278794644
>50	0.8189794001686944	8.734009549395894
>100	2.0186714458434474	65.14900866357613
>500	0.01749955982719228	1.5480017437852953
>1k	0.0011666373218128187	0.34769460353677734
>5k	3.888791072709396E-4	0.48141896240108406
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8543	0.4789965910110343	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3304	0.18525163721180585	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6068897461200325E-5	0.0	0.0	0.0348187853234054	0.0
2	5.6068897461200325E-5	0.0	0.0	0.1029424957387638	0.0
3	5.6068897461200325E-5	0.0	0.0	0.14527451332197003	0.0
4	5.6068897461200325E-5	0.0	0.0	0.21216470799318202	0.0
5	5.6068897461200325E-5	0.0	0.0	0.34073068987171434	0.0
6	5.6068897461200325E-5	0.0	0.0	0.4543823450255674	0.0
7	5.6068897461200325E-5	0.0	0.0	0.520319368439939	0.0
8	5.6068897461200325E-5	0.0	0.0	0.6589216829640262	0.0
9	5.6068897461200325E-5	0.0	0.0	0.7007490804700817	0.0
10	5.6068897461200325E-5	0.0	0.0	0.7891136628689334	0.0
11	5.6068897461200325E-5	0.0	0.0	0.9705526150533776	0.0
12	5.6068897461200325E-5	0.0	0.0	1.110612720911456	0.0
13	5.6068897461200325E-5	0.0	0.0	1.1657845160132772	0.0
14	1.1213779492240065E-4	0.0	0.0	1.1886045572799857	0.0
15	1.1213779492240065E-4	0.0	0.0	1.2260585807840674	0.0
16	1.1213779492240065E-4	0.0	0.0	1.3050596573068987	0.0
17	1.1213779492240065E-4	0.0	0.0	1.4007692652731676	0.0
18	1.1213779492240065E-4	0.0	0.0	1.513075266887952	0.0
19	1.1213779492240065E-4	0.0	0.0	1.5887122095631112	0.0
20	1.1213779492240065E-4	0.0	0.0	1.6671525971113303	0.0
21	1.1213779492240065E-4	0.0	0.0	1.7704315062348615	0.0
22	1.1213779492240065E-4	0.0	0.0	1.8751682066923836	0.0
23	1.1213779492240065E-4	0.0	0.0	1.9851193146137975	0.0
24	1.1213779492240065E-4	0.0	0.0	2.068325558446219	0.0
25	1.1213779492240065E-4	0.0	0.0	2.1424486408899255	0.0
26	1.1213779492240065E-4	0.0	0.0	2.225206333542657	0.0
27	1.1213779492240065E-4	0.0	0.0	2.3135709159415088	0.0
28	1.1213779492240065E-4	0.0	0.0	2.3968332286713916	0.0
29	1.1213779492240065E-4	0.0	0.0	2.5012335157441465	0.0
30	1.1213779492240065E-4	0.0	0.0	2.6159504799497624	0.0
31	1.1213779492240065E-4	0.0	0.0	2.7248923477168745	0.0
32	1.1213779492240065E-4	0.0	0.0	2.8236296761460484	0.0
33	1.1213779492240065E-4	0.0	0.0	2.9198999730869293	0.0
34	1.1213779492240065E-4	0.0	0.0	3.034000179420472	0.0
35	1.1213779492240065E-4	0.0	0.0	3.1715932537902574	0.0
36	1.1213779492240065E-4	0.0	0.0	3.282946084148201	0.0
37	1.1213779492240065E-4	0.0	0.0	3.4083722077689065	0.0
38	1.1213779492240065E-4	0.0	0.0	3.555833408091863	0.0
39	1.1213779492240065E-4	0.0	0.0	3.8139185431057685	0.0
40	1.1213779492240065E-4	0.0	0.0	3.972313178433659	0.0
41	1.1213779492240065E-4	0.0	0.0	4.1211761011931465	0.0
42	1.1213779492240065E-4	0.0	0.0	4.276318740468287	0.0
43	1.1213779492240065E-4	0.0	0.0	4.395296940880955	0.0
44	1.1213779492240065E-4	0.0	0.0	4.516686103884453	0.0
45	1.1213779492240065E-4	0.0	0.0	4.649008701892886	0.0
46	1.1213779492240065E-4	0.0	0.0	4.815421189557728	0.0
47	1.1213779492240065E-4	0.0	0.0	4.986094913429622	0.0
48	1.1213779492240065E-4	0.0	0.0	5.138434107831704	0.0
49	1.1213779492240065E-4	0.0	0.0	5.288642684130259	5.6068897461200325E-5
50	1.1213779492240065E-4	0.0	0.0	5.4502893155109	5.6068897461200325E-5
51	1.1213779492240065E-4	0.0	0.0	5.617598905535122	5.6068897461200325E-5
52	1.1213779492240065E-4	0.0	0.0	5.820287969857361	5.6068897461200325E-5
53	1.1213779492240065E-4	0.0	0.0	5.997353548039832	5.6068897461200325E-5
54	1.1213779492240065E-4	0.0	0.0	6.176325468735983	5.6068897461200325E-5
55	1.1213779492240065E-4	0.0	0.0	6.348681259531713	5.6068897461200325E-5
56	1.1213779492240065E-4	0.0	0.0	6.5251861487395715	5.6068897461200325E-5
57	1.1213779492240065E-4	0.0	0.0	6.703709518256033	5.6068897461200325E-5
58	1.1213779492240065E-4	0.0	0.0	6.870234143715798	5.6068897461200325E-5
59	1.6820669238360096E-4	0.0	0.0	7.060756257288957	5.6068897461200325E-5
60	1.6820669238360096E-4	0.0	0.0	7.264118148380731	5.6068897461200325E-5
61	1.6820669238360096E-4	0.0	0.0	7.463499147752759	5.6068897461200325E-5
62	2.242755898448013E-4	0.0	0.0	7.71765945994438	5.6068897461200325E-5
63	2.803444873060016E-4	0.0	0.0	7.961334888310756	5.6068897461200325E-5
64	3.364133847672019E-4	0.0	0.0	8.186339373822554	5.6068897461200325E-5
65	3.364133847672019E-4	0.0	0.0	8.389252713734637	5.6068897461200325E-5
66	3.364133847672019E-4	0.0	0.0	8.577812415896654	5.6068897461200325E-5
67	3.364133847672019E-4	0.0	0.0	8.778370862115368	5.6068897461200325E-5
68	3.364133847672019E-4	0.0	0.0	9.030961245178075	5.6068897461200325E-5
69	3.9248228222840226E-4	0.0	0.0	9.400287072755	5.6068897461200325E-5
70	3.9248228222840226E-4	0.0	0.0	9.622880595675966	5.6068897461200325E-5
71	3.9248228222840226E-4	0.0	0.0	9.847380461110612	5.6068897461200325E-5
72	3.9248228222840226E-4	0.0	0.0	10.060890822642863	5.6068897461200325E-5
73	4.485511796896026E-4	0.0	0.0	10.294978469543375	5.6068897461200325E-5
74	4.485511796896026E-4	0.0	0.0	10.555530636045573	5.6068897461200325E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTACG	15	0.0013099099	80.00001	80
TAGCGCG	20	4.0915198E-5	80.0	1
GCGATAA	50	0.0	80.0	9
TATAGCG	215	0.0	74.4186	1
TCGATAG	65	0.0	73.84615	1
GTTAGCG	155	0.0	72.258064	1
TACGTAG	100	0.0	68.0	1
CGTTTTT	4915	0.0	67.222786	1
TAACGCG	155	0.0	67.09678	1
TGTAGCG	155	0.0	67.09678	1
GGATACG	110	0.0	65.454544	1
GCGTAAG	215	0.0	65.11628	1
GCGAGGG	1515	0.0	64.9505	3
TTAAGCG	340	0.0	64.70588	80
GTACGGG	700	0.0	64.57143	3
AAGTACT	6680	0.0	64.43114	80
ATACCGG	75	0.0	63.999996	2
CGAGGGA	790	0.0	63.291138	4
CCGCGAG	70	0.0	62.857143	1
TAGTAGG	1395	0.0	62.7957	2
>>END_MODULE