FastQCFastQC Report
Thu 26 May 2016
SRR1547743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547743_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences886915
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57940.6532756803075831No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20060.2261772548665881No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT9110.10271559281329103No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC9060.10215184093176911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTCC351.2115015E-745.00000436
CGTTAGG1950.045.0000042
CCACTAG351.2115015E-745.00000410
GCGATAT950.045.0000049
TATAGCG351.2115015E-745.0000041
CCACGTA351.2115015E-745.00000419
TCCGGAC253.890082E-545.015
GATACGT207.0324144E-445.022
CCTTACG253.890082E-545.017
CTACTTA253.890082E-545.023
CTCGTCT253.890082E-545.040
CTATCGT207.0324144E-445.031
ACGTTAG453.8380676E-1045.01
ACGCACG207.0324144E-445.041
CACGCGA207.0324144E-445.041
CAACCGA207.0324144E-445.044
ACCGAAT207.0324144E-445.02
TGAACGA406.8121153E-945.027
CACGATT207.0324144E-445.010
CGACATA207.0324144E-445.030