##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547743_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	886915
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53773585969343	31.0	31.0	33.0	30.0	34.0
2	31.946355625961903	31.0	31.0	34.0	30.0	34.0
3	32.081685392625	33.0	31.0	34.0	30.0	34.0
4	35.73806509079224	37.0	35.0	37.0	33.0	37.0
5	35.61393932902251	37.0	35.0	37.0	33.0	37.0
6	35.674517851203326	37.0	35.0	37.0	33.0	37.0
7	36.06685533562968	37.0	35.0	37.0	35.0	37.0
8	36.09478022132899	37.0	35.0	37.0	35.0	37.0
9	37.89650642959021	39.0	38.0	39.0	35.0	39.0
10	37.28086231487798	39.0	37.0	39.0	34.0	39.0
11	36.93872017047857	39.0	37.0	39.0	33.0	39.0
12	36.19053798842054	38.0	35.0	39.0	32.0	39.0
13	35.81885637293315	38.0	35.0	39.0	31.0	39.0
14	36.740423828664525	39.0	35.0	41.0	31.0	41.0
15	37.035356263001525	39.0	35.0	41.0	32.0	41.0
16	37.20468026812039	39.0	35.0	41.0	32.0	41.0
17	37.17146738977242	39.0	35.0	41.0	32.0	41.0
18	37.08350856620984	38.0	35.0	41.0	32.0	41.0
19	37.022560222794745	38.0	35.0	41.0	32.0	41.0
20	36.86443007503537	38.0	35.0	41.0	32.0	41.0
21	36.724193411995515	38.0	35.0	41.0	31.0	41.0
22	36.68193569846039	38.0	35.0	41.0	31.0	41.0
23	36.650686931667636	38.0	35.0	40.0	31.0	41.0
24	36.54390330527728	38.0	35.0	40.0	31.0	41.0
25	36.418871030482066	38.0	35.0	40.0	31.0	41.0
26	36.28239910250701	38.0	35.0	40.0	31.0	41.0
27	36.247372070604285	38.0	35.0	40.0	31.0	41.0
28	36.32102625392512	38.0	35.0	40.0	31.0	41.0
29	36.31678683977608	38.0	35.0	40.0	31.0	41.0
30	36.34060084675533	38.0	35.0	40.0	31.0	41.0
31	36.183240784066115	38.0	35.0	40.0	30.0	41.0
32	36.0043048093673	38.0	35.0	40.0	30.0	41.0
33	35.90251940715852	38.0	35.0	40.0	30.0	41.0
34	35.71080543231313	38.0	35.0	40.0	29.0	41.0
35	35.61794196738131	38.0	35.0	40.0	29.0	41.0
36	35.45113793317285	38.0	34.0	40.0	28.0	41.0
37	35.24452737861013	37.0	34.0	40.0	27.0	41.0
38	35.33291578110642	37.0	34.0	40.0	28.0	41.0
39	35.262274287840434	37.0	34.0	40.0	27.0	41.0
40	35.117607662515574	37.0	34.0	40.0	27.0	41.0
41	35.09074375785729	37.0	34.0	40.0	27.0	41.0
42	35.07570398516205	37.0	34.0	40.0	27.0	41.0
43	35.00652260926921	37.0	34.0	40.0	27.0	41.0
44	34.866548654606135	36.0	34.0	40.0	26.0	41.0
45	34.81954527773237	36.0	34.0	40.0	26.0	41.0
46	34.668953620132704	36.0	34.0	40.0	26.0	41.0
47	34.680701081839864	36.0	34.0	40.0	26.0	41.0
48	34.557402907832206	36.0	34.0	40.0	26.0	41.0
49	34.572720046453156	36.0	34.0	40.0	26.0	41.0
50	34.446004408539714	35.0	34.0	40.0	26.0	41.0
51	33.7458065316293	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	4.0
13	7.0
14	10.0
15	21.0
16	71.0
17	185.0
18	338.0
19	753.0
20	1303.0
21	2023.0
22	3057.0
23	4553.0
24	6558.0
25	9399.0
26	12311.0
27	14981.0
28	16427.0
29	18676.0
30	21862.0
31	26392.0
32	32999.0
33	43811.0
34	77696.0
35	121998.0
36	60575.0
37	76769.0
38	115318.0
39	218656.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.37704289588066	23.783677128022415	29.116093424961804	13.723186551135115
2	30.347553034958253	25.102292778902154	29.805449225686793	14.744704960452806
3	30.144602357610367	25.077600446491488	30.853463973436014	13.92433322246213
4	25.33974507139918	27.59306134184223	31.19859287530372	15.868600711454874
5	23.715012148853045	31.597616457044925	30.870940281763193	13.816431112338837
6	22.360203627179605	40.18603812090223	26.82196151829657	10.631796733621599
7	88.48401481539945	3.431670453200138	6.562748403172796	1.5215663282276204
8	88.69846603113038	2.7700512450460306	6.42259968542645	2.1088830383971406
9	84.17266592627253	5.019308501942126	7.901997372916232	2.9060281988691137
10	48.06187740651585	26.97338527367335	13.808313085244922	11.156424234565883
11	39.75420417965645	23.27066291583748	22.23832047039457	14.7368124341115
12	38.41067069561345	20.744716235490436	25.07038442240801	15.774228646488108
13	24.281131788277342	31.783429077194548	26.334992643037946	17.600446491490164
14	17.488259867067306	36.19264529295367	28.673773698719717	17.64532114125931
15	16.098385978363204	26.3697197589397	41.496084743182834	16.03580951951427
16	20.735921706138694	22.646364082240126	39.0836776917743	17.534036519846886
17	20.999757586690944	23.27133941809531	26.664223741846737	29.06467925336701
18	23.232440538270296	25.982647717086753	31.633358326333415	19.151553418309533
19	28.42662487386052	26.685420812591964	25.663000400263837	19.224953913283684
20	29.215764757614878	26.84045258001049	26.911372566705943	17.032410095668695
21	24.864051233770994	26.517310001522134	29.989908841320755	18.62872992338612
22	24.21280506023689	23.38792330719404	28.950575872546974	23.448695760022098
23	21.410507207567804	27.757902391999234	28.52099693882728	22.31059346160568
24	21.97200408156362	25.615081490334475	34.44805872039598	17.964855707705922
25	21.333949702057133	26.4135796553221	31.81161667127064	20.44085397135013
26	19.55294475795313	32.572005209067385	26.478974873578643	21.396075159400844
27	19.702339006556436	32.196659206350105	29.746931780384816	18.354070006708646
28	16.797551061826667	28.745257437296694	35.065479781038775	19.391711719837865
29	17.931368845943524	26.17872062148007	35.01203610267049	20.87787442990591
30	20.343212145470535	29.170439106340517	31.361855420192462	19.124493327996483
31	26.60119628149259	26.607848553694545	26.16158256428181	20.629372600531052
32	27.22402936019799	27.650113032252243	26.740330245852196	18.38552736169757
33	25.867191331751073	26.688690573504786	27.03460872800663	20.40950936673751
34	20.51707322573189	27.982839392726472	28.127272624772388	23.37281475676925
35	21.24532790628189	27.47298219107806	29.05983098718592	22.221858915454128
36	27.736930822006617	26.350439444591643	27.223578358692773	18.689051374708963
37	21.16426038571904	30.25712723316214	29.049683453318526	19.528928927800298
38	21.33496445544387	30.583652322939624	25.89571717695608	22.185666044660422
39	21.0578240304877	28.865111087308254	28.15241595868826	21.924648923515782
40	23.869141913261135	26.42744795160754	27.326406701882366	22.37700343324896
41	19.157641938629972	24.441575573758477	29.460996826076908	26.939785661534643
42	22.207426867287168	25.657475631824923	26.634344892126077	25.500752608761836
43	22.67815968835796	25.18110529193891	27.823410360632078	24.317324659071048
44	21.463725385183473	27.692732674495304	28.602177209766438	22.24136473055479
45	20.122559659042864	31.372679456317687	25.295885175016767	23.20887570962268
46	22.48975380955334	30.541258181449184	25.83550847600954	21.13347953298794
47	21.236420626553844	27.684840148153995	27.82814587643686	23.250593348855304
48	22.827328436208656	25.743053167439946	30.441925099925022	20.987693296426375
49	22.116888315114753	24.692445161035725	30.379574141828698	22.811092382020824
50	19.371642152855685	28.274975617731123	29.814469255791142	22.53891297362205
51	19.076799918819727	30.46357317217546	26.10915363930027	24.350473269704537
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	846.0
1	1201.5
2	1557.0
3	5287.0
4	9017.0
5	6151.5
6	3286.0
7	3440.5
8	3595.0
9	3865.5
10	4136.0
11	4154.5
12	4173.0
13	4010.5
14	3848.0
15	3810.5
16	3773.0
17	3586.5
18	3400.0
19	3556.0
20	3712.0
21	4033.5
22	4355.0
23	3767.0
24	3179.0
25	3975.5
26	6083.5
27	7395.0
28	10289.5
29	13184.0
30	13310.0
31	13436.0
32	16313.5
33	19191.0
34	22590.5
35	25990.0
36	27531.0
37	29072.0
38	32691.5
39	36311.0
40	44789.5
41	53268.0
42	62217.5
43	71167.0
44	73701.5
45	76236.0
46	75624.5
47	75013.0
48	73496.0
49	71979.0
50	66951.0
51	61923.0
52	58492.5
53	55062.0
54	50321.0
55	45580.0
56	43703.5
57	41827.0
58	39228.5
59	36630.0
60	33071.0
61	29512.0
62	26079.5
63	22647.0
64	20064.5
65	17482.0
66	14589.0
67	11696.0
68	9437.5
69	7179.0
70	5868.5
71	4558.0
72	4092.0
73	3626.0
74	2547.5
75	1189.0
76	909.0
77	635.0
78	361.0
79	376.0
80	391.0
81	257.5
82	124.0
83	74.5
84	25.0
85	16.0
86	7.0
87	5.0
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	5.5
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	886915.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.615917692334335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.79114727261334	16.044084128611793
2	7.653367980965998	3.00255672767552
3	2.4219924120978327	1.4252881142150817
4	1.14832776306871	0.901020115367129
5	0.6574773592413171	0.6448510881725505
6	0.4231429977594318	0.49802029296819766
7	0.3190271360155339	0.43806070291913046
8	0.24495606388668092	0.38440303899514594
9	0.19019853740692283	0.33578269694789137
>10	2.3712799692465523	11.015479555447241
>50	1.2541944525912825	18.096807540289298
>100	1.5173848209215286	45.46952953166375
>500	0.006348890464106814	0.861019521548915
>1k	5.771718603733467E-4	0.227114419490746
>5k	5.771718603733467E-4	0.6559825256876284
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5794	0.6532756803075831	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2006	0.2261772548665881	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	911	0.10271559281329103	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	906	0.10215184093176911	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11252487555177215	0.0
2	0.0	0.0	0.0	0.3753460027172841	0.0
3	0.0	0.0	0.0	0.5130142121849331	0.0
4	0.0	0.0	0.0	0.7562167738734829	0.0
5	0.0	0.0	0.0	1.2221013287631848	0.0
6	0.0	0.0	0.0	1.5754610081011144	0.0
7	0.0	0.0	0.0	1.7749164237835644	0.0
8	0.0	0.0	0.0	2.1502624265008485	0.0
9	0.0	0.0	0.0	2.269326823878275	0.0
10	0.0	0.0	0.0	2.5239171735735666	0.0
11	0.0	0.0	0.0	2.962065135892391	0.0
12	0.0	0.0	0.0	3.270437415084873	0.0
13	0.0	0.0	0.0	3.399874847082302	0.0
14	0.0	0.0	0.0	3.46132380216819	0.0
15	0.0	0.0	0.0	3.5642648957340897	0.0
16	0.0	0.0	0.0	3.76450956405067	0.0
17	0.0	0.0	0.0	3.99587333622726	0.0
18	0.0	0.0	0.0	4.272675510054515	0.0
19	0.0	0.0	0.0	4.436389056448476	0.0
20	1.1275037630438092E-4	0.0	0.0	4.592548327630044	0.0
21	1.1275037630438092E-4	0.0	0.0	4.811509558413151	0.0
22	1.1275037630438092E-4	0.0	0.0	5.0235930162416915	0.0
23	1.1275037630438092E-4	0.0	0.0	5.243117998906321	0.0
24	1.1275037630438092E-4	0.0	0.0	5.412130812986589	0.0
25	1.1275037630438092E-4	0.0	0.0	5.553407034495978	0.0
26	1.1275037630438092E-4	0.0	0.0	5.694006753747541	0.0
27	1.1275037630438092E-4	0.0	0.0	5.862794067075199	0.0
28	1.1275037630438092E-4	0.0	0.0	6.016360079601766	0.0
29	1.1275037630438092E-4	0.0	0.0	6.192814418518122	0.0
30	1.1275037630438092E-4	0.0	0.0	6.384151807106656	0.0
31	1.1275037630438092E-4	0.0	0.0	6.560380645270404	0.0
32	1.1275037630438092E-4	0.0	0.0	6.719133175106972	0.0
33	1.1275037630438092E-4	0.0	0.0	6.868301922957668	0.0
34	1.1275037630438092E-4	0.0	0.0	7.052310537086417	0.0
35	1.1275037630438092E-4	0.0	0.0	7.271271767869525	0.0
36	1.1275037630438092E-4	0.0	0.0	7.4303625488350065	0.0
37	1.1275037630438092E-4	0.0	0.0	7.609410146406364	0.0
38	1.1275037630438092E-4	0.0	0.0	7.81213532300164	0.0
39	1.1275037630438092E-4	0.0	0.0	8.157264224869351	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACTCC	35	1.2115015E-7	45.000004	36
CGTTAGG	195	0.0	45.000004	2
CCACTAG	35	1.2115015E-7	45.000004	10
GCGATAT	95	0.0	45.000004	9
TATAGCG	35	1.2115015E-7	45.000004	1
CCACGTA	35	1.2115015E-7	45.000004	19
TCCGGAC	25	3.890082E-5	45.0	15
GATACGT	20	7.0324144E-4	45.0	22
CCTTACG	25	3.890082E-5	45.0	17
CTACTTA	25	3.890082E-5	45.0	23
CTCGTCT	25	3.890082E-5	45.0	40
CTATCGT	20	7.0324144E-4	45.0	31
ACGTTAG	45	3.8380676E-10	45.0	1
ACGCACG	20	7.0324144E-4	45.0	41
CACGCGA	20	7.0324144E-4	45.0	41
CAACCGA	20	7.0324144E-4	45.0	44
ACCGAAT	20	7.0324144E-4	45.0	2
TGAACGA	40	6.8121153E-9	45.0	27
CACGATT	20	7.0324144E-4	45.0	10
CGACATA	20	7.0324144E-4	45.0	30
>>END_MODULE