FastQCFastQC Report
Thu 26 May 2016
SRR1547742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547742_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1431063
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92310.6450449770555174No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT21370.149329554324303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA207.0338673E-445.012
CGTTAGA207.0338673E-445.010
CGAATTT207.0338673E-445.031
TAGATCG351.2122109E-745.01
AATCGTC351.2122109E-745.044
TACGTCG207.0338673E-445.01
CGGCATA253.8912884E-544.99999621
TACGGAT253.8912884E-544.99999613
TCTAGCG302.165878E-644.9999961
ATCGGTA253.8912884E-544.99999627
CGGTATG302.165878E-644.99999620
CGGTCTA1400.043.39285731
TCGATAG750.042.0000041
ACACGCG3500.041.78571336
ACGGGAT5850.041.1538435
AATTATC22150.041.03837645
TACGGGA3600.040.6254
CGTTTTT47850.040.344831
TAACGGG2400.040.3124963
TTACACG3750.040.234
Produced by FastQC (version 0.11.5)