##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547742_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1431063
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.520620685462486	31.0	31.0	33.0	30.0	34.0
2	31.93787415368855	31.0	31.0	34.0	30.0	34.0
3	32.0920958755834	33.0	31.0	34.0	30.0	34.0
4	35.776357155485115	37.0	35.0	37.0	35.0	37.0
5	35.656522459178944	37.0	35.0	37.0	33.0	37.0
6	35.69105063858125	37.0	35.0	37.0	33.0	37.0
7	36.05047646399914	37.0	35.0	37.0	35.0	37.0
8	36.060823318050986	37.0	35.0	37.0	35.0	37.0
9	37.862780324835455	39.0	38.0	39.0	35.0	39.0
10	37.24488230077921	39.0	37.0	39.0	34.0	39.0
11	36.96357812339499	39.0	37.0	39.0	33.0	39.0
12	36.57396914042219	39.0	35.0	39.0	32.0	39.0
13	36.43900932383829	39.0	35.0	39.0	32.0	39.0
14	37.51216962495711	40.0	36.0	41.0	32.0	41.0
15	37.665035012434814	40.0	36.0	41.0	32.0	41.0
16	37.74138245486048	40.0	36.0	41.0	33.0	41.0
17	37.678099426789736	40.0	36.0	41.0	33.0	41.0
18	37.56366910471447	39.0	36.0	41.0	32.0	41.0
19	37.51811136197358	39.0	36.0	41.0	32.0	41.0
20	37.38423046364835	39.0	35.0	41.0	32.0	41.0
21	37.292590193443615	39.0	35.0	41.0	32.0	41.0
22	37.22120130280777	39.0	35.0	41.0	32.0	41.0
23	37.12971686082304	39.0	35.0	41.0	32.0	41.0
24	37.044023219103565	39.0	35.0	41.0	32.0	41.0
25	36.960186239180246	39.0	35.0	41.0	31.0	41.0
26	36.8537401917316	39.0	35.0	41.0	31.0	41.0
27	36.807656965486494	39.0	35.0	41.0	31.0	41.0
28	36.812407280462146	39.0	35.0	41.0	31.0	41.0
29	36.79520887619902	39.0	35.0	41.0	31.0	41.0
30	36.792697456366355	39.0	35.0	41.0	31.0	41.0
31	36.68589293413358	39.0	35.0	41.0	31.0	41.0
32	36.52403423189615	39.0	35.0	41.0	30.0	41.0
33	36.40778428343127	39.0	35.0	41.0	30.0	41.0
34	36.21712810686881	39.0	35.0	41.0	30.0	41.0
35	36.11452325998226	39.0	35.0	41.0	30.0	41.0
36	35.99976590827937	39.0	35.0	41.0	29.0	41.0
37	35.78027592076659	38.0	35.0	41.0	28.0	41.0
38	35.83519523598891	38.0	35.0	41.0	29.0	41.0
39	35.71721231001011	38.0	35.0	41.0	28.0	41.0
40	35.58937027929588	38.0	35.0	41.0	27.0	41.0
41	35.50465493133426	38.0	35.0	40.0	27.0	41.0
42	35.48508556227085	38.0	35.0	40.0	27.0	41.0
43	35.43539033571548	38.0	34.0	40.0	27.0	41.0
44	35.29430220751986	38.0	34.0	40.0	27.0	41.0
45	35.25461842001366	38.0	34.0	40.0	27.0	41.0
46	35.05009213430855	38.0	34.0	40.0	26.0	41.0
47	35.08176788862545	37.0	34.0	40.0	26.0	41.0
48	34.911328152569105	37.0	34.0	40.0	26.0	41.0
49	34.86559291938929	37.0	34.0	40.0	26.0	41.0
50	34.77140908541413	37.0	34.0	40.0	26.0	41.0
51	34.08498298118252	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	7.0
13	14.0
14	32.0
15	62.0
16	129.0
17	297.0
18	621.0
19	1176.0
20	2028.0
21	3139.0
22	4655.0
23	6783.0
24	9665.0
25	13985.0
26	18680.0
27	22776.0
28	24957.0
29	28145.0
30	32019.0
31	39142.0
32	48321.0
33	62792.0
34	102295.0
35	132277.0
36	107831.0
37	143287.0
38	213901.0
39	411815.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.7894537137778	24.228073816456718	25.85735219204186	15.125120277723623
2	29.912519574609924	26.478289215778762	29.66333417885865	13.945857030752665
3	28.298963777276054	26.471860428227128	31.41538842105484	13.81378737344198
4	26.666471007915092	27.53170195861398	31.162499484648826	14.639327548822099
5	24.37397934262852	31.690219088887073	29.396469617340397	14.53933195114401
6	23.394288022260376	39.393304138252475	26.4872336158506	10.725174223636555
7	87.0678649367638	3.8562942372208626	7.172360685728021	1.903480140287325
8	86.78045620633054	3.5503678035138915	7.262852858329786	2.4063231318257827
9	82.24885976368616	5.84313898130271	8.604792381607238	3.3032088734038965
10	41.37029606663019	33.003857971312236	15.12036856518546	10.505477396872115
11	30.689773965227246	23.815583241268904	28.16703387621649	17.32760891728736
12	31.141466168854897	21.341059058895382	29.438606127053806	18.078868645195914
13	25.283443146807656	25.547233070801216	28.303086586684163	20.86623719570697
14	18.75843341627867	26.87331025957627	30.639391836697616	23.728864487447442
15	18.289481315637396	28.82717252839323	33.262826304642076	19.620519851327302
16	23.130637854517936	26.802593596508327	30.723594977998875	19.343173570974862
17	23.687566515240768	28.00107332800862	27.105235758313924	21.20612439843669
18	23.927248485915715	27.55413283691913	29.148891418477035	19.369727258688123
19	25.020142369693016	28.031889581381115	26.28214131732845	20.66582673159742
20	25.95692852096658	26.89441345349576	29.370125564003818	17.77853246153384
21	25.89739235798843	26.32895966145446	29.91342799024222	17.860219990314892
22	26.22861467314856	21.691218346082596	30.36616836575329	21.71399861501555
23	23.22979491468929	25.762807088157547	30.49306704177244	20.51433095538072
24	21.164756548104453	25.861684635826656	33.38944546815898	19.58411334790991
25	21.747330480908246	29.325822832398014	28.25955251445953	20.667294172234207
26	20.966861696515107	30.296569752694325	26.360963842961493	22.375604707829076
27	19.99164257618288	30.81534495686074	29.59792825333336	19.59508421362302
28	18.213244280650116	28.269475208289226	32.2215723556545	21.295708155406157
29	19.45616650000734	26.500929728460594	31.461507983925234	22.581395787606834
30	21.137923347888947	28.26297654261203	30.567696879871814	20.031403229627205
31	24.01648285225738	27.49410752706205	28.010716509336063	20.478693111344505
32	24.213958435093353	26.789037240149455	27.424928182756453	21.572076142000736
33	23.39980839417971	27.282306928486026	27.5894911684531	21.72839350888116
34	21.160633738696337	26.169218266421534	30.121874438791302	22.548273556090823
35	22.19021804071519	26.468506278200195	29.244344937993645	22.096930743090976
36	24.657474898030348	28.058792659722176	26.857238290697193	20.42649415155028
37	21.37851373419619	28.469536281770964	28.96371438573983	21.18823559829302
38	21.835796187868738	27.980459280968063	28.60838411726109	21.575360413902114
39	21.281383139666108	28.463736397349383	28.292115720971054	21.962764742013455
40	24.431209527463153	26.893644794114586	27.865719398796557	20.809426279625704
41	20.156205561879524	25.76259745378086	30.651550630545266	23.429646353794347
42	20.777002829365305	26.066357665595437	28.937090819901012	24.21954868513825
43	21.867450978747964	27.383141063670852	27.933431302465372	22.815976655115815
44	21.189004257674192	27.520102189770824	28.031120921999943	23.259772630555048
45	20.92947689933986	29.365583485842343	26.489958862747482	23.214980752070314
46	21.059310456632588	29.68276029776467	26.82977618735164	22.428153058251105
47	20.835420942334473	26.94668229141554	30.992625761409524	21.225271004840458
48	21.05518764722448	26.472070062603812	31.630333535281117	20.842408754890595
49	21.908050169699028	27.541764408694796	28.869658428734446	21.680526992871734
50	20.7049584819117	26.69435238001402	30.614934492751196	21.98575464532309
51	20.085139508183776	27.9650860933446	28.976641838968654	22.97313255950297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1565.0
1	2916.5
2	4268.0
3	9963.0
4	15658.0
5	11176.5
6	6695.0
7	6720.0
8	6745.0
9	6803.0
10	6861.0
11	6786.5
12	6712.0
13	6725.0
14	6738.0
15	6281.5
16	5825.0
17	5903.0
18	5981.0
19	6182.5
20	6384.0
21	6852.0
22	7320.0
23	7708.5
24	8097.0
25	10370.5
26	14178.0
27	15712.0
28	19881.0
29	24050.0
30	29365.5
31	34681.0
32	38268.0
33	41855.0
34	49958.0
35	58061.0
36	59244.5
37	60428.0
38	62760.0
39	65092.0
40	73513.0
41	81934.0
42	82092.5
43	82251.0
44	86366.5
45	90482.0
46	91080.5
47	91679.0
48	93345.0
49	95011.0
50	94447.5
51	93884.0
52	86571.5
53	79259.0
54	76798.5
55	74338.0
56	73065.0
57	71792.0
58	68506.5
59	65221.0
60	57911.0
61	50601.0
62	48034.0
63	45467.0
64	40469.0
65	35471.0
66	30183.5
67	24896.0
68	21125.0
69	17354.0
70	14924.5
71	12495.0
72	10115.0
73	7735.0
74	6071.0
75	3368.5
76	2330.0
77	1956.0
78	1582.0
79	1304.0
80	1026.0
81	628.5
82	231.0
83	150.0
84	69.0
85	54.0
86	39.0
87	64.5
88	90.0
89	60.0
90	30.0
91	17.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	5.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1431063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.338251983053308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.0077716980309	15.858869536652287
2	6.40554747414048	2.4774418228867847
3	1.9277204954323206	1.1183623408069971
4	0.9704324748107045	0.7506587092170974
5	0.6472297676278818	0.6258146168660508
6	0.4793061779064644	0.5561366187233632
7	0.3695903155083524	0.5003061456257685
8	0.2882251757835751	0.4459016861730088
9	0.25867646766247	0.4502115642368687
>10	3.349753365365956	16.684780800330536
>50	2.1822449284829637	30.965410415627815
>100	1.1087710966433004	28.146816134716317
>500	0.0036388943112678047	0.54754784277428
>1k	7.277788622535609E-4	0.22215763264855443
>5k	3.6388943112678043E-4	0.6495841327142242
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9231	0.6450449770555174	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2137	0.149329554324303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037105284673001816	0.0
2	0.0	0.0	0.0	0.12067952284420742	0.0
3	0.0	0.0	0.0	0.16994360136485956	0.0
4	0.0	0.0	0.0	0.255474427051779	0.0
5	0.0	0.0	0.0	0.42982035032699467	0.0
6	0.0	0.0	0.0	0.5674103795570146	0.0
7	0.0	0.0	0.0	0.6549676708852091	0.0
8	0.0	0.0	0.0	0.8182728503217538	0.0
9	0.0	0.0	0.0	0.876271694537557	0.0
10	0.0	0.0	0.0	0.9941560923593161	0.0
11	0.0	0.0	0.0	1.2105686472223796	0.0
12	0.0	0.0	0.0	1.3767388298069336	0.0
13	0.0	0.0	0.0	1.4512987897807434	0.0
14	0.0	0.0	0.0	1.4872161463192046	0.0
15	0.0	0.0	0.0	1.5486390186875072	0.0
16	0.0	0.0	0.0	1.669458297782837	0.0
17	0.0	0.0	0.0	1.8178794364748443	0.0
18	0.0	0.0	0.0	1.9662306970412904	0.0
19	0.0	0.0	0.0	2.0714671541364704	0.0
20	1.397562511224174E-4	0.0	0.0	2.181804714397619	0.0
21	1.397562511224174E-4	0.0	0.0	2.3281993874483513	0.0
22	2.096343766836261E-4	0.0	0.0	2.4815818730552044	0.0
23	2.096343766836261E-4	0.0	0.0	2.6359426524199145	0.0
24	2.096343766836261E-4	0.0	0.0	2.7535475377394287	0.0
25	2.096343766836261E-4	0.0	0.0	2.8589237510857313	0.0
26	2.096343766836261E-4	0.0	0.0	2.96059642377729	0.0
27	2.096343766836261E-4	0.0	0.0	3.084839731025119	0.0
28	2.096343766836261E-4	0.0	0.0	3.2027940069724394	0.0
29	2.096343766836261E-4	0.0	0.0	3.3317890267584307	0.0
30	2.096343766836261E-4	0.0	0.0	3.4834944373518146	0.0
31	2.096343766836261E-4	0.0	0.0	3.6065498164651033	0.0
32	2.096343766836261E-4	0.0	0.0	3.723665554905689	0.0
33	2.096343766836261E-4	0.0	0.0	3.8447643465032635	0.0
34	2.096343766836261E-4	0.0	0.0	3.975087050674918	0.0
35	2.096343766836261E-4	0.0	0.0	4.120503429967793	0.0
36	2.096343766836261E-4	0.0	0.0	4.242650393448786	0.0
37	2.795125022448348E-4	0.0	0.0	4.379751275799878	0.0
38	2.795125022448348E-4	0.0	0.0	4.528591683245252	0.0
39	2.795125022448348E-4	0.0	0.0	4.794058682252284	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	20	7.0338673E-4	45.0	12
CGTTAGA	20	7.0338673E-4	45.0	10
CGAATTT	20	7.0338673E-4	45.0	31
TAGATCG	35	1.2122109E-7	45.0	1
AATCGTC	35	1.2122109E-7	45.0	44
TACGTCG	20	7.0338673E-4	45.0	1
CGGCATA	25	3.8912884E-5	44.999996	21
TACGGAT	25	3.8912884E-5	44.999996	13
TCTAGCG	30	2.165878E-6	44.999996	1
ATCGGTA	25	3.8912884E-5	44.999996	27
CGGTATG	30	2.165878E-6	44.999996	20
CGGTCTA	140	0.0	43.392857	31
TCGATAG	75	0.0	42.000004	1
ACACGCG	350	0.0	41.785713	36
ACGGGAT	585	0.0	41.153843	5
AATTATC	2215	0.0	41.038376	45
TACGGGA	360	0.0	40.625	4
CGTTTTT	4785	0.0	40.34483	1
TAACGGG	240	0.0	40.312496	3
TTACACG	375	0.0	40.2	34
>>END_MODULE