FastQCFastQC Report
Thu 26 May 2016
SRR1547741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547741_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1017054
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71430.7023225905409153No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11730.11533310915644596No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG10830.1064840214973836No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT453.8380676E-1045.0000044
CTAACGG253.8904873E-545.0000042
CTTATCG253.8904873E-545.00000425
AGGCACG453.8380676E-1045.00000410
ACGTGCG253.8904873E-545.0000041
CAGTCGA253.8904873E-545.00000414
CGCGTTC253.8904873E-545.00000425
GCGTCGC253.8904873E-545.00000421
TGTCGTT253.8904873E-545.00000438
TATCGAA253.8904873E-545.00000427
CGATACT253.8904873E-545.00000410
TCGGTCT253.8904873E-545.00000442
ATCGACG253.8904873E-545.0000041
TGCAACG253.8904873E-545.0000041
GCATACG253.8904873E-545.00000424
CGAACAC207.032903E-445.033
CGAGTAG207.032903E-445.045
CAACGAA207.032903E-445.014
CGACGGA207.032903E-445.029
CGTGCGG850.045.02