##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547741_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1017054
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50133621223652	31.0	31.0	33.0	30.0	34.0
2	31.902924525148123	31.0	31.0	34.0	30.0	34.0
3	32.03597842395782	33.0	31.0	34.0	30.0	34.0
4	35.71007340809829	37.0	35.0	37.0	33.0	37.0
5	35.58627270528409	37.0	35.0	37.0	33.0	37.0
6	35.639500950785305	37.0	35.0	37.0	33.0	37.0
7	36.061126547852915	37.0	35.0	37.0	35.0	37.0
8	36.093608598953445	37.0	35.0	37.0	35.0	37.0
9	37.9085004336053	39.0	38.0	39.0	35.0	39.0
10	37.260189724439414	39.0	37.0	39.0	34.0	39.0
11	36.88330609780798	39.0	37.0	39.0	33.0	39.0
12	36.320576881856816	38.0	35.0	39.0	32.0	39.0
13	36.04340084203985	38.0	35.0	39.0	31.0	39.0
14	36.93901110462178	39.0	35.0	41.0	31.0	41.0
15	37.22822485335095	39.0	35.0	41.0	32.0	41.0
16	37.36888601785156	39.0	35.0	41.0	32.0	41.0
17	37.3267623941305	39.0	35.0	41.0	32.0	41.0
18	37.22044650529864	39.0	35.0	41.0	32.0	41.0
19	37.18753773152655	39.0	35.0	41.0	32.0	41.0
20	37.046123411342954	39.0	35.0	41.0	32.0	41.0
21	36.95486473677897	39.0	35.0	41.0	32.0	41.0
22	36.94486920065208	39.0	35.0	41.0	32.0	41.0
23	36.887551693420406	38.0	35.0	41.0	32.0	41.0
24	36.787381004351786	38.0	35.0	41.0	31.0	41.0
25	36.698845882322864	38.0	35.0	41.0	31.0	41.0
26	36.57593598766634	38.0	35.0	41.0	31.0	41.0
27	36.54778212366305	38.0	35.0	41.0	31.0	41.0
28	36.599181557714736	38.0	35.0	41.0	31.0	41.0
29	36.59632920179263	38.0	35.0	41.0	31.0	41.0
30	36.59692897328952	38.0	35.0	41.0	31.0	41.0
31	36.43944569314904	38.0	35.0	41.0	30.0	41.0
32	36.27528135182596	38.0	35.0	41.0	30.0	41.0
33	36.19641533291251	38.0	35.0	41.0	30.0	41.0
34	36.04416088034657	38.0	35.0	41.0	30.0	41.0
35	35.97656269971899	38.0	35.0	41.0	30.0	41.0
36	35.827021967368495	38.0	35.0	41.0	29.0	41.0
37	35.59025676119459	38.0	34.0	40.0	28.0	41.0
38	35.68594292928399	38.0	35.0	41.0	29.0	41.0
39	35.58953310247047	38.0	35.0	40.0	28.0	41.0
40	35.466992903031695	38.0	34.0	40.0	27.0	41.0
41	35.397353532850765	38.0	34.0	40.0	27.0	41.0
42	35.427812092573255	38.0	34.0	40.0	27.0	41.0
43	35.38104663075904	38.0	34.0	40.0	27.0	41.0
44	35.26497118147119	38.0	34.0	40.0	27.0	41.0
45	35.24380514702268	38.0	34.0	40.0	27.0	41.0
46	35.084010288539254	37.0	34.0	40.0	26.0	41.0
47	35.12325304261131	37.0	34.0	40.0	27.0	41.0
48	34.96069431908237	37.0	34.0	40.0	26.0	41.0
49	34.93746939690518	37.0	34.0	40.0	26.0	41.0
50	34.809635476582365	37.0	34.0	40.0	26.0	41.0
51	34.138349586157666	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	2.0
13	4.0
14	9.0
15	26.0
16	96.0
17	195.0
18	466.0
19	805.0
20	1434.0
21	2303.0
22	3486.0
23	4874.0
24	7262.0
25	10001.0
26	13515.0
27	16307.0
28	18116.0
29	20474.0
30	23910.0
31	29489.0
32	36606.0
33	47907.0
34	81782.0
35	114415.0
36	69419.0
37	89908.0
38	140776.0
39	283313.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.5563303423417	23.621361304316192	28.49504549414289	14.327262859199216
2	29.41535061068537	25.21341049737772	31.816403062177624	13.554835829759284
3	29.373563252295355	24.947741221213427	32.50899165629357	13.16970387019765
4	25.91160351367774	27.66411616295693	31.738137797993026	14.6861425253723
5	23.291781950614222	31.99004182668767	30.18001010762457	14.538166115073537
6	22.49949363553951	40.99605330690406	25.792140830280395	10.712312227276035
7	89.25691261230966	3.4525207117812817	6.025540433447978	1.265026242461069
8	89.9135149166121	2.6853048117405764	5.84629724675386	1.5548830248934669
9	85.92739421898935	4.9944250747747905	6.9602990598335985	2.117881646402256
10	46.336871001933034	33.97046764478582	11.452980864339553	8.239680488941591
11	33.550037657784145	23.033388590969604	27.12756648122912	16.289007270017127
12	33.669008725200435	22.218092647981326	27.077028358376253	17.03587026844199
13	25.4081887490733	29.41957850812248	27.402084845052478	17.77014789775174
14	17.157496062155992	32.54773099560102	30.47271826274711	19.822054679495878
15	17.00686492555951	27.767650488567963	38.68811292222439	16.537371663648145
16	23.300434391880863	25.828323766486346	34.90542291756387	15.965818924068929
17	23.223054036462173	25.025121576632113	27.43168012711223	24.320144259793484
18	24.649625290299237	26.172946569208715	30.941326615892567	18.23610152459948
19	26.316990051659005	27.887998080731208	26.77881410426585	19.016197763343932
20	26.43704267423362	29.27759981279263	27.579263244626144	16.7060942683476
21	24.62484784485386	25.777883966829684	31.26520322421425	18.3320649641022
22	24.22467243627182	22.66792127065033	31.522711675092964	21.584694617984887
23	20.85002369589029	27.423519301826648	31.01959188007716	20.7068651222059
24	20.228522772635475	27.077028358376253	34.12984954584516	18.564599323143117
25	21.207920130101254	28.34461100393883	30.47114509160772	19.9763237743522
26	20.040725467870928	31.67776735551898	26.685013775079792	21.596493401530303
27	18.523401903930374	31.219876230760608	31.259893771618813	18.99682809369021
28	17.160445758042346	28.158878486294732	34.195037824933586	20.485637930729343
29	18.7293889999941	27.17033707158125	33.95581748855027	20.144456439874382
30	20.885813339311383	27.86843176468506	32.28442147614581	18.961333419857745
31	25.667860310268676	26.64155492235417	28.16054998063033	19.530034786746818
32	26.1603612000936	27.093251685751202	28.516479950917063	18.22990716323814
33	24.51364430993831	26.713724148373636	28.150029398635667	20.622602143052386
34	19.39631523989877	27.685648942927315	30.209605389684324	22.708430427489592
35	20.18103266886517	28.29485946665565	29.90637665256712	21.61773121191205
36	26.798282097115788	26.815881949237703	27.70865657084088	18.677179382805633
37	20.589958841910068	31.324983727511025	28.828066159712268	19.256991270866642
38	21.596001785549245	29.959274532129072	26.787269899140064	21.65745378318162
39	20.197944258613603	28.77801965284046	29.11300678233407	21.911029306211862
40	24.18691632892649	26.96808625697357	27.682600923844753	21.16239649025519
41	19.65765829542974	25.587038642982574	29.466478672715507	25.28882438887217
42	20.773233279648867	26.949306526497118	28.371551559700865	23.905908634153153
43	21.134472702531035	27.5730688832648	28.444704017682444	22.84775439652172
44	20.687397129355965	28.443819108916536	28.096541579896446	22.772242181831054
45	19.393857159993473	31.054988230713416	26.14226973199063	23.408884877302484
46	21.695898152900437	29.60826072165293	27.597256389532905	21.09858473591373
47	20.448865055346126	28.52306760506325	29.50738112233962	21.520686217251
48	21.70012605033754	28.047969920967812	29.59892001801281	20.652984010681834
49	23.101526565944386	25.538958600035006	30.115903383694477	21.24361145032614
50	20.024502140495983	28.747441138818587	29.513280514112328	21.714776206573102
51	19.64556454229569	29.71228666324502	27.603647397286675	23.038501397172617
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	645.0
1	1337.5
2	2030.0
3	6396.5
4	10763.0
5	7354.0
6	3945.0
7	3941.5
8	3938.0
9	4202.0
10	4466.0
11	4430.0
12	4394.0
13	4236.0
14	4078.0
15	4026.0
16	3974.0
17	3977.0
18	3980.0
19	3815.0
20	3650.0
21	4187.0
22	4724.0
23	4909.0
24	5094.0
25	6756.0
26	10223.5
27	12029.0
28	13419.5
29	14810.0
30	17083.0
31	19356.0
32	23058.0
33	26760.0
34	31542.5
35	36325.0
36	38574.0
37	40823.0
38	43247.5
39	45672.0
40	54536.0
41	63400.0
42	71502.0
43	79604.0
44	81239.0
45	82874.0
46	81384.5
47	79895.0
48	79933.5
49	79972.0
50	74972.5
51	69973.0
52	68546.5
53	67120.0
54	62150.0
55	57180.0
56	54037.0
57	50894.0
58	46700.5
59	42507.0
60	34881.0
61	27255.0
62	24261.5
63	21268.0
64	17771.5
65	14275.0
66	11590.5
67	8906.0
68	7001.5
69	5097.0
70	3924.5
71	2752.0
72	2455.0
73	2158.0
74	1667.0
75	842.5
76	509.0
77	364.5
78	220.0
79	151.0
80	82.0
81	66.5
82	51.0
83	28.0
84	5.0
85	3.0
86	1.0
87	2.0
88	3.0
89	1.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1017054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.44111105614978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.89126154287952	15.920571101833284
2	7.027745873932502	2.7325437601904032
3	2.2068010137148613	1.2870799075936357
4	1.0533160726830466	0.8191053898503455
5	0.6382326732835234	0.6203976140484168
6	0.4692080711221586	0.5473155731476625
7	0.3779808187095108	0.5143856951538209
8	0.3101846289460818	0.482426705540111
9	0.2474139564164573	0.4329001983180373
>10	2.8078187386841047	13.303628890394027
>50	1.493910629992669	21.514256026467997
>100	1.4735853152988692	40.77232318139482
>500	0.0010162657346888752	0.12456999724212348
>1k	0.0010162657346888752	0.22286273892008768
>5k	5.081328673444376E-4	0.7056332199052243
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7143	0.7023225905409153	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1173	0.11533310915644596	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1083	0.1064840214973836	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.83231962118039E-5	0.0	0.0	0.04621190221954783	0.0
2	9.83231962118039E-5	0.0	0.0	0.15603891238813278	0.0
3	9.83231962118039E-5	0.0	0.0	0.2169009708432394	0.0
4	9.83231962118039E-5	0.0	0.0	0.3275145665815188	0.0
5	9.83231962118039E-5	0.0	0.0	0.580598473630702	0.0
6	9.83231962118039E-5	0.0	0.0	0.7778348052315807	0.0
7	9.83231962118039E-5	0.0	0.0	0.9172570974599186	0.0
8	9.83231962118039E-5	0.0	0.0	1.2066222639112574	0.0
9	9.83231962118039E-5	0.0	0.0	1.2972762508185407	0.0
10	9.83231962118039E-5	0.0	0.0	1.4762244679240237	0.0
11	9.83231962118039E-5	0.0	0.0	1.7900721102321018	0.0
12	9.83231962118039E-5	0.0	0.0	2.0293907698116325	0.0
13	9.83231962118039E-5	0.0	0.0	2.1135554257689364	0.0
14	9.83231962118039E-5	0.0	0.0	2.1562276929248596	0.0
15	9.83231962118039E-5	0.0	0.0	2.2217109416019207	0.0
16	9.83231962118039E-5	0.0	0.0	2.3530707317408908	0.0
17	9.83231962118039E-5	0.0	0.0	2.5208101044782283	0.0
18	9.83231962118039E-5	0.0	0.0	2.6898276787663193	0.0
19	1.966463924236078E-4	0.0	0.0	2.81469813795531	0.0
20	2.949695886354117E-4	0.0	0.0	2.950482471923811	0.0
21	2.949695886354117E-4	0.0	0.0	3.1207782477626558	0.0
22	2.949695886354117E-4	0.0	0.0	3.3152615298696038	0.0
23	2.949695886354117E-4	0.0	0.0	3.4893919103607085	0.0
24	2.949695886354117E-4	0.0	0.0	3.626552769076175	0.0
25	2.949695886354117E-4	0.0	0.0	3.7497517339295654	0.0
26	2.949695886354117E-4	0.0	0.0	3.875802071473098	0.0
27	2.949695886354117E-4	0.0	0.0	4.045212938546036	0.0
28	2.949695886354117E-4	0.0	0.0	4.180702302925901	0.0
29	2.949695886354117E-4	0.0	0.0	4.359847166423808	0.0
30	2.949695886354117E-4	0.0	0.0	4.555805296473934	0.0
31	2.949695886354117E-4	0.0	0.0	4.715482167121903	0.0
32	2.949695886354117E-4	0.0	0.0	4.856674276882053	0.0
33	2.949695886354117E-4	0.0	0.0	5.006617151105054	0.0
34	2.949695886354117E-4	0.0	0.0	5.184877105837055	0.0
35	2.949695886354117E-4	0.0	0.0	5.371592855443271	0.0
36	2.949695886354117E-4	0.0	0.0	5.531957988464723	0.0
37	2.949695886354117E-4	0.0	0.0	5.698320836455095	0.0
38	2.949695886354117E-4	0.0	0.0	5.923087662995279	0.0
39	2.949695886354117E-4	0.0	0.0	6.375177719177153	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	45	3.8380676E-10	45.000004	4
CTAACGG	25	3.8904873E-5	45.000004	2
CTTATCG	25	3.8904873E-5	45.000004	25
AGGCACG	45	3.8380676E-10	45.000004	10
ACGTGCG	25	3.8904873E-5	45.000004	1
CAGTCGA	25	3.8904873E-5	45.000004	14
CGCGTTC	25	3.8904873E-5	45.000004	25
GCGTCGC	25	3.8904873E-5	45.000004	21
TGTCGTT	25	3.8904873E-5	45.000004	38
TATCGAA	25	3.8904873E-5	45.000004	27
CGATACT	25	3.8904873E-5	45.000004	10
TCGGTCT	25	3.8904873E-5	45.000004	42
ATCGACG	25	3.8904873E-5	45.000004	1
TGCAACG	25	3.8904873E-5	45.000004	1
GCATACG	25	3.8904873E-5	45.000004	24
CGAACAC	20	7.032903E-4	45.0	33
CGAGTAG	20	7.032903E-4	45.0	45
CAACGAA	20	7.032903E-4	45.0	14
CGACGGA	20	7.032903E-4	45.0	29
CGTGCGG	85	0.0	45.0	2
>>END_MODULE