FastQCFastQC Report
Thu 26 May 2016
SRR1547740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547740_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1333689
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77330.579820332926192No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACC253.891143E-545.00000430
ACGTACG253.891143E-545.0000041
CCGATTA207.033694E-445.012
TCTCGCG406.8157533E-945.032
GTATACG207.033694E-445.01
TAACGCG302.1657652E-644.9999961
CGTTTTT36800.042.8600581
GTCGAAG501.0822987E-940.5000041
AGGGCGA5950.040.4621856
CGTTAGG1900.040.263162
TTTCGCG451.9286745E-840.01
ACCGACG451.9286745E-840.01
GCGTCAT850.039.70588337
TAATGCG800.039.3751
CGGACGG403.458772E-739.3752
GGGCGAT11350.039.25117
TTACACG5800.039.18103434
TAAGGGA18800.039.135644
GCGTATG1150.039.1304361
ATAGGGT6100.039.0983624
Produced by FastQC (version 0.11.5)