FastQCFastQC Report
Thu 26 May 2016
SRR1547739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547739_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences998111
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62410.6252811561038802No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG11700.1172214312836949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGA207.0328417E-445.018
CGGTTAT207.0328417E-445.031
ATTCGCG207.0328417E-445.01
CAATTCG207.0328417E-445.016
TATTCCG207.0328417E-445.014
AACGCGT253.8904356E-545.012
GCGATAC253.8904356E-545.09
ACGACTA253.8904356E-545.013
AATAGCG800.042.18751
CGTTTTT28600.041.931821
CGACGGT1700.041.0294128
ACGTTAG501.0804797E-940.51
CGGTCTA1400.040.17857431
AACGTTA451.9279469E-840.045
TACCGGT451.9279469E-840.040
GTAGGGC3750.039.64
GTTAACG403.4576078E-739.3751
ACGTAGG1500.039.02
TCGACGG750.039.02
TTAGGGA13250.038.886794
Produced by FastQC (version 0.11.5)