FastQCFastQC Report
Thu 26 May 2016
SRR1547738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547738_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences365493
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.5083544691690401No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.026966E-445.025
TTTAGCG207.026966E-445.01
CTAGACG207.026966E-445.01
CACGCGT207.026966E-445.026
CGATTGA207.026966E-445.010
TACGCGG253.8855673E-545.02
AGACCGG207.026966E-445.02
CGTAAGG351.2088822E-745.02
CGCGTGG207.026966E-445.028
TATAACG207.026966E-445.01
CGATAGT207.026966E-445.010
GCGTAAG207.026966E-445.01
GCGATCA302.161425E-644.9999969
TATACGG302.161425E-644.9999962
GGCACCG1200.043.1249968
CGAGGGC800.042.18754
AGTACGG603.6379788E-1241.2499962
CGTTTTT9950.040.929651
TCGTCCC556.002665E-1140.90909238
TAGTGCG556.002665E-1140.9090921