##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547738_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	365493
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53382964926825	31.0	31.0	33.0	30.0	34.0
2	31.952981315647634	31.0	31.0	34.0	30.0	34.0
3	32.09189505681368	33.0	31.0	34.0	30.0	34.0
4	35.76151937246404	37.0	35.0	37.0	35.0	37.0
5	35.64756643766091	37.0	35.0	37.0	33.0	37.0
6	35.707329552139164	37.0	35.0	37.0	33.0	37.0
7	36.121567307718614	37.0	35.0	37.0	35.0	37.0
8	36.15424372012597	37.0	35.0	37.0	35.0	37.0
9	37.97604331683507	39.0	38.0	39.0	35.0	39.0
10	37.26943334072062	39.0	37.0	39.0	34.0	39.0
11	36.96248628564706	39.0	37.0	39.0	33.0	39.0
12	36.686264853225644	39.0	35.0	39.0	33.0	39.0
13	36.61514721212171	39.0	35.0	39.0	32.0	39.0
14	37.67647807208346	40.0	36.0	41.0	33.0	41.0
15	37.82369840188458	40.0	36.0	41.0	33.0	41.0
16	37.858339284199694	40.0	36.0	41.0	33.0	41.0
17	37.80049959917153	40.0	36.0	41.0	33.0	41.0
18	37.70470843490847	39.0	36.0	41.0	33.0	41.0
19	37.70633363703272	39.0	36.0	41.0	33.0	41.0
20	37.62208304947017	39.0	35.0	41.0	33.0	41.0
21	37.517531115507	39.0	35.0	41.0	32.0	41.0
22	37.48625007866088	39.0	35.0	41.0	32.0	41.0
23	37.40033324851639	39.0	35.0	41.0	32.0	41.0
24	37.36312049751979	39.0	35.0	41.0	32.0	41.0
25	37.25310744665425	39.0	35.0	41.0	32.0	41.0
26	37.13589042745005	39.0	35.0	41.0	32.0	41.0
27	37.12035798223222	39.0	35.0	41.0	31.0	41.0
28	37.156550193847764	39.0	35.0	41.0	32.0	41.0
29	37.150429693591946	39.0	35.0	41.0	32.0	41.0
30	37.188528918474496	39.0	35.0	41.0	32.0	41.0
31	37.086513284796155	39.0	35.0	41.0	31.0	41.0
32	36.9656546089802	39.0	35.0	41.0	31.0	41.0
33	36.90084899026794	39.0	35.0	41.0	31.0	41.0
34	36.765626154262876	39.0	35.0	41.0	31.0	41.0
35	36.69068354250287	39.0	35.0	41.0	30.0	41.0
36	36.61093919719393	39.0	35.0	41.0	30.0	41.0
37	36.401846273389644	39.0	35.0	41.0	30.0	41.0
38	36.51581562437584	39.0	35.0	41.0	30.0	41.0
39	36.41595598274112	39.0	35.0	41.0	30.0	41.0
40	36.316698267819085	39.0	35.0	41.0	30.0	41.0
41	36.27864008339421	39.0	35.0	41.0	30.0	41.0
42	36.272462126497636	39.0	35.0	41.0	30.0	41.0
43	36.2375722654059	39.0	35.0	41.0	30.0	41.0
44	36.11054931284594	39.0	35.0	41.0	29.0	41.0
45	36.08747363150594	39.0	35.0	41.0	29.0	41.0
46	35.90575195694582	38.0	35.0	40.0	29.0	41.0
47	35.94534505448805	38.0	35.0	40.0	29.0	41.0
48	35.79510961906247	38.0	35.0	40.0	28.0	41.0
49	35.76175467108809	38.0	35.0	40.0	28.0	41.0
50	35.66427811202951	38.0	34.0	40.0	28.0	41.0
51	34.990801465417945	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	16.0
17	39.0
18	105.0
19	189.0
20	322.0
21	530.0
22	852.0
23	1222.0
24	1892.0
25	2673.0
26	3793.0
27	4624.0
28	5382.0
29	6239.0
30	7385.0
31	9130.0
32	11807.0
33	15229.0
34	23717.0
35	31806.0
36	26687.0
37	35634.0
38	56696.0
39	119431.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52675153833315	23.713176449343763	30.44162268497618	13.3184493273469
2	29.53380776102415	25.292686864043905	31.58692505738824	13.586580317543701
3	28.86731072824924	24.747669586011224	33.147009655451676	13.238010030287858
4	25.38324947399813	28.048143192892887	31.982281466402913	14.586325866706066
5	23.581846984757572	32.394874867644525	30.02191560440282	14.00136254319508
6	21.658143931621126	41.68369845660519	25.98627059888972	10.671887012883968
7	91.66523025064775	2.4038764080297024	4.7872325872178125	1.1436607541047297
8	92.76456731045465	1.7278032684620497	4.55248116927	0.9551482518133042
9	88.50374699378646	4.844962831025492	5.222261438659563	1.4290287365284697
10	38.80512075470666	42.49876194619323	11.230858046528935	7.465259252571185
11	26.557827372890863	24.665315067593635	30.60386929435037	18.17298826516513
12	27.19176564257045	23.65489899943364	30.044351054602963	19.10898430339295
13	24.145469270273303	25.996667514836126	30.242439663687133	19.615423551203442
14	18.5270306134454	29.73463240062053	31.252582128795904	20.485754857138165
15	19.198999707244734	29.53161893661438	33.84168780250237	17.42769355363851
16	24.359974062430744	28.499861830459132	30.312482044799765	16.82768206231036
17	23.504690924313188	27.541703945082396	28.17372699340343	20.779878137200985
18	23.07704935525441	27.995611407058412	29.97211984908056	18.955219388606622
19	24.464490427997255	28.496031387742036	27.978100811780255	19.061377372480457
20	25.970948828021328	30.068975329212872	27.80053243153768	16.15954341122812
21	26.280120275901314	25.804598172878823	30.634239233035927	17.281042318183932
22	24.866413310241235	23.03901853113466	31.726189010459844	20.36837914816426
23	20.8638742739259	27.184378360187473	31.55518710344658	20.396560262440047
24	20.588082398294905	27.875773270623515	32.30184983022931	19.234294500852272
25	21.4515736279491	29.717942614496035	29.18195423715365	19.648529520401212
26	20.63103807733664	31.631795957788523	26.96494871310805	20.772217251766794
27	19.63484936784015	30.977063856216127	30.952713184657437	18.43537359128629
28	18.38174739324693	29.421083303921	32.37079779913706	19.826371503695007
29	19.705986161157668	28.330227938701974	31.899106138831662	20.0646797613087
30	21.58290309253529	27.62132243298777	31.16284032799534	19.632934146481603
31	23.151742988237807	27.997526628416956	29.453368463965113	19.397361919380124
32	24.03985849250191	27.81065574443286	28.82107181259285	19.328413950472374
33	23.082521416278833	27.305037305776036	29.106987001119037	20.505454276826097
34	20.444714399454984	28.091646078037062	30.501815356244855	20.961824166263103
35	20.821465800986612	28.432555479858713	29.90809673509479	20.837881984059887
36	23.556401900994	28.59124524956702	28.241033344003853	19.611319505435123
37	20.971673876107065	29.726150706032673	29.34228562516929	19.95988979269097
38	21.195754775057253	29.944759543958433	28.022150903026873	20.837334777957444
39	20.909565983479848	28.859376239763822	28.76416237793884	21.46689539881749
40	22.056783577250453	28.241580550106292	28.353484198055778	21.348151674587477
41	19.952502510307994	27.227607642280425	29.452821257862666	23.36706858954891
42	20.846363678647744	27.833364797684222	28.93844752156676	22.381824002101272
43	21.1035505467957	27.953750140221562	28.627634455379447	22.315064857603293
44	20.74294172528612	28.321746244114117	29.157603565594965	21.777708465004803
45	20.989731677487665	29.318755762764265	27.751283882317857	21.94022867743021
46	21.286864591113922	29.30808524376664	28.255807908769796	21.149242256349645
47	20.63404771090007	28.0599081240954	29.97102543687567	21.33501872812886
48	21.47893393307122	27.599434188890076	29.863499437745727	21.058132440292972
49	21.697816374048205	27.44676368630863	29.54393107391934	21.31148886572383
50	20.331989942351836	28.72640515687031	29.40877116661605	21.5328337341618
51	20.103804997633333	28.914917659161738	28.46566144905648	22.515615894148453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	210.0
1	343.0
2	476.0
3	1746.0
4	3016.0
5	2090.0
6	1164.0
7	1223.0
8	1282.0
9	1262.0
10	1242.0
11	1282.0
12	1322.0
13	1332.5
14	1343.0
15	1299.0
16	1255.0
17	1283.5
18	1312.0
19	1297.5
20	1283.0
21	1490.5
22	1698.0
23	1825.5
24	1953.0
25	2682.0
26	3922.0
27	4433.0
28	5529.5
29	6626.0
30	7703.0
31	8780.0
32	10415.5
33	12051.0
34	12907.5
35	13764.0
36	15593.5
37	17423.0
38	18759.5
39	20096.0
40	21474.0
41	22852.0
42	24870.5
43	26889.0
44	27566.0
45	28243.0
46	28915.5
47	29588.0
48	29201.0
49	28814.0
50	28333.5
51	27853.0
52	25848.0
53	23843.0
54	21664.0
55	19485.0
56	17542.0
57	15599.0
58	13887.0
59	12175.0
60	10997.0
61	9819.0
62	8150.5
63	6482.0
64	5300.5
65	4119.0
66	3358.0
67	2597.0
68	1971.0
69	1345.0
70	1007.0
71	669.0
72	552.5
73	436.0
74	364.0
75	236.0
76	180.0
77	103.0
78	26.0
79	23.5
80	21.0
81	20.5
82	20.0
83	12.0
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	365493.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.226447666521132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.24429435915164	18.98067555600736
2	7.1610284334811025	4.042607886322483
3	3.626930885653091	3.0712612450192833
4	2.6481324797338597	2.9898949141289046
5	2.110094329308025	2.9780233578817987
6	1.828409185259562	3.0965697708429345
7	1.5897387541184966	3.141087442259638
8	1.394016123688135	3.1478498529255834
9	1.2512192181978263	3.1785726403625785
>10	11.1081902381554	54.01882224779009
>50	0.028958784324552976	0.5459692389662348
>100	0.007988630158497372	0.28496381032259344
>500	0.0	0.0
>1k	9.985787698121714E-4	0.523702037170503
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1858	0.5083544691690401	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03693641191486567	0.0
2	0.0	0.0	0.0	0.13379189204718012	0.0
3	0.0	0.0	0.0	0.19261654805974396	0.0
4	0.0	0.0	0.0	0.2744238603748909	0.0
5	0.0	0.0	0.0	0.46649320233219244	0.0
6	0.0	0.0	0.0	0.6506280558040783	0.0
7	0.0	0.0	0.0	0.7559652305242508	0.0
8	0.0	0.0	0.0	0.9504969999425433	0.0
9	0.0	0.0	0.0	1.0180769535941865	0.0
10	0.0	0.0	0.0	1.1852484178903564	0.0
11	0.0	0.0	0.0	1.4670595606482204	0.0
12	0.0	0.0	0.0	1.709745467081449	0.0
13	0.0	0.0	0.0	1.810978596033303	0.0
14	0.0	0.0	0.0	1.868708839840982	0.0
15	0.0	0.0	0.0	1.9434024728243768	0.0
16	0.0	0.0	0.0	2.079383189281327	0.0
17	0.0	0.0	0.0	2.278019004467938	0.0
18	0.0	0.0	0.0	2.4777492318594336	0.0
19	0.0	0.0	0.0	2.6170131849310385	0.0
20	0.0	0.0	0.0	2.776523763793014	0.0
21	0.0	0.0	0.0	2.941506403679414	0.0
22	0.0	0.0	0.0	3.1579264171954047	0.0
23	0.0	0.0	0.0	3.372978415455289	0.0
24	0.0	0.0	0.0	3.5272905363440614	0.0
25	0.0	0.0	0.0	3.688442733513364	0.0
26	0.0	0.0	0.0	3.826612274380084	0.0
27	0.0	0.0	0.0	3.9858492501908382	0.0
28	0.0	0.0	0.0	4.143991813796707	0.0
29	0.0	0.0	0.0	4.325937842858823	0.0
30	0.0	0.0	0.0	4.531140131274744	0.0
31	0.0	0.0	0.0	4.7002268169294625	0.0
32	0.0	0.0	0.0	4.869860708686623	0.0
33	0.0	0.0	0.0	5.035937760777908	0.0
34	0.0	0.0	0.0	5.223355850864449	0.0
35	0.0	0.0	0.0	5.428558139280369	0.0
36	0.0	0.0	0.0	5.611324977496149	0.0
37	0.0	0.0	0.0	5.825829769653591	0.0
38	0.0	0.0	0.0	6.05292030216721	0.0
39	0.0	0.0	0.0	6.430492512852504	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.026966E-4	45.0	25
TTTAGCG	20	7.026966E-4	45.0	1
CTAGACG	20	7.026966E-4	45.0	1
CACGCGT	20	7.026966E-4	45.0	26
CGATTGA	20	7.026966E-4	45.0	10
TACGCGG	25	3.8855673E-5	45.0	2
AGACCGG	20	7.026966E-4	45.0	2
CGTAAGG	35	1.2088822E-7	45.0	2
CGCGTGG	20	7.026966E-4	45.0	28
TATAACG	20	7.026966E-4	45.0	1
CGATAGT	20	7.026966E-4	45.0	10
GCGTAAG	20	7.026966E-4	45.0	1
GCGATCA	30	2.161425E-6	44.999996	9
TATACGG	30	2.161425E-6	44.999996	2
GGCACCG	120	0.0	43.124996	8
CGAGGGC	80	0.0	42.1875	4
AGTACGG	60	3.6379788E-12	41.249996	2
CGTTTTT	995	0.0	40.92965	1
TCGTCCC	55	6.002665E-11	40.909092	38
TAGTGCG	55	6.002665E-11	40.909092	1
>>END_MODULE