FastQCFastQC Report
Thu 26 May 2016
SRR1547737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547737_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2134932
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174430.8170283643694506No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA253.8919334E-545.00000436
CGACTGA253.8919334E-545.00000437
TCGCACG406.8193913E-945.01
GTCGATC207.034647E-445.023
CAACGCG351.2125747E-745.01
CTATACG207.034647E-445.01
CGATTCG351.2125747E-745.010
TCGGCGA207.034647E-445.027
ACCTCGA207.034647E-445.016
ATATCCG207.034647E-445.031
CGTACAA207.034647E-445.032
TAGGCGT207.034647E-445.017
ACGTACC207.034647E-445.028
TCGCGGA406.8193913E-945.039
CGCTTAC207.034647E-445.026
CGCCACT207.034647E-445.031
GTCGAAT1200.044.99999611
CGTTAAT302.16638E-644.99999641
TTTAGCG3250.043.6153831
ACGCTAG1000.042.7500041