##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547737_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2134932
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.474020249825287	31.0	31.0	33.0	30.0	34.0
2	31.870166356586534	31.0	31.0	34.0	30.0	34.0
3	32.00089557887558	33.0	31.0	34.0	30.0	34.0
4	35.68081465826546	37.0	35.0	37.0	33.0	37.0
5	35.55005452164284	37.0	35.0	37.0	33.0	37.0
6	35.61345747780257	37.0	35.0	37.0	33.0	37.0
7	36.08705616853371	37.0	35.0	37.0	35.0	37.0
8	36.12818066336539	37.0	35.0	37.0	35.0	37.0
9	37.91916182810506	39.0	38.0	39.0	35.0	39.0
10	37.181041831777314	39.0	37.0	39.0	33.0	39.0
11	36.83111405890211	39.0	37.0	39.0	32.0	39.0
12	36.50497954969995	39.0	35.0	39.0	32.0	39.0
13	36.369122295230014	39.0	35.0	39.0	32.0	39.0
14	37.41398414563087	40.0	36.0	41.0	32.0	41.0
15	37.602242600701096	40.0	36.0	41.0	32.0	41.0
16	37.682988497994316	40.0	36.0	41.0	33.0	41.0
17	37.61132017319521	39.0	35.0	41.0	33.0	41.0
18	37.52782149501717	39.0	36.0	41.0	32.0	41.0
19	37.50665454450071	39.0	36.0	41.0	32.0	41.0
20	37.387576278776095	39.0	35.0	41.0	32.0	41.0
21	37.30828710235267	39.0	35.0	41.0	32.0	41.0
22	37.23647544746156	39.0	35.0	41.0	32.0	41.0
23	37.152983326869425	39.0	35.0	41.0	32.0	41.0
24	37.09227132292738	39.0	35.0	41.0	32.0	41.0
25	37.004007621788425	39.0	35.0	41.0	32.0	41.0
26	36.88664603837499	39.0	35.0	41.0	31.0	41.0
27	36.840074063248856	39.0	35.0	41.0	31.0	41.0
28	36.89968720315214	39.0	35.0	41.0	31.0	41.0
29	36.869795384583675	39.0	35.0	41.0	31.0	41.0
30	36.88675517534048	39.0	35.0	41.0	31.0	41.0
31	36.772324832828396	39.0	35.0	41.0	31.0	41.0
32	36.61041709993574	39.0	35.0	41.0	30.0	41.0
33	36.53555335720294	39.0	35.0	41.0	30.0	41.0
34	36.40818958168223	39.0	35.0	41.0	30.0	41.0
35	36.34859424094069	39.0	35.0	41.0	30.0	41.0
36	36.23775932910275	39.0	35.0	41.0	30.0	41.0
37	36.00276308566268	38.0	35.0	41.0	29.0	41.0
38	36.11234971418293	38.0	35.0	41.0	30.0	41.0
39	36.03391770791763	38.0	35.0	41.0	30.0	41.0
40	35.94268997794777	38.0	35.0	41.0	29.0	41.0
41	35.901351893175054	38.0	35.0	41.0	29.0	41.0
42	35.89687259360017	38.0	35.0	40.0	29.0	41.0
43	35.84577963138873	38.0	35.0	40.0	29.0	41.0
44	35.709411353616886	38.0	35.0	40.0	28.0	41.0
45	35.658059835161026	38.0	35.0	40.0	28.0	41.0
46	35.46973252543875	38.0	34.0	40.0	27.0	41.0
47	35.50269376261164	38.0	34.0	40.0	28.0	41.0
48	35.354452975551446	38.0	34.0	40.0	27.0	41.0
49	35.34625552476613	38.0	34.0	40.0	27.0	41.0
50	35.22945320975094	38.0	34.0	40.0	27.0	41.0
51	34.538505676059	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	2.0
13	3.0
14	12.0
15	35.0
16	112.0
17	306.0
18	640.0
19	1286.0
20	2284.0
21	3607.0
22	5467.0
23	8107.0
24	12450.0
25	18488.0
26	24584.0
27	29727.0
28	33667.0
29	39206.0
30	47304.0
31	58690.0
32	73543.0
33	95386.0
34	148630.0
35	218664.0
36	161809.0
37	207081.0
38	315605.0
39	627874.0
40	360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.625535614249074	23.38496027039737	30.637135046924215	13.352369068429345
2	27.940234162024836	26.001905447105578	32.42566976372081	13.63219062714878
3	27.878405494882273	25.923261256096215	32.74769407175498	13.450639177266535
4	25.77046950441513	27.38194940166713	32.321872546760275	14.525708547157473
5	22.517860053622314	33.10882969574675	30.771799757556686	13.601510493074255
6	21.888753365446767	40.50958063301314	27.111636342515826	10.49002965902427
7	90.81474257728115	2.4025589573813124	5.53211999258056	1.2505784727569778
8	91.25316403520112	1.8725654962312617	5.272439590581808	1.6018308779858093
9	86.50786067190899	4.992711711661074	6.622178130263634	1.877249486166304
10	40.20469972814122	40.415994514110984	11.812694736881548	7.5666110208662385
11	27.90646259459318	23.98085746993347	31.355846462557118	16.756833472916234
12	28.87740686822812	23.2391476637195	30.058849649543873	17.824595818508506
13	23.27409022863492	27.88163744793745	30.564720562528457	18.279551760899178
14	16.962085911869792	32.30074775215323	31.58765712444237	19.149509211534607
15	16.51687266854401	28.91473826801041	37.428358373943524	17.140030689502055
16	20.914764498354046	28.135884421611557	34.13846436326778	16.810886716766625
17	20.832935194188853	27.965761907170815	28.439406969402302	22.761895929238026
18	21.911330196933672	27.98688670177786	31.583535213299534	18.518247887988938
19	24.81104784602039	28.155697699036786	28.692576625391347	18.34067782955148
20	24.799572070679535	29.380420547352326	28.655479425105813	17.164527956862326
21	24.246580218948424	28.525732903905137	30.46022074707766	16.76746613006878
22	23.51283319562403	24.2551050806302	30.558069296820694	21.673992426925075
23	19.251245472923728	28.97230450431208	31.257014274927723	20.519435747836464
24	19.127260259343153	28.187642510393772	34.46479794204218	18.22029928822089
25	19.320240644666903	30.519332700057895	30.55638306044408	19.604043594831126
26	19.13667507911259	33.821779803759554	27.197587557823855	19.843957559303995
27	18.501245004524737	32.2862273833546	31.272330922015314	17.940196690105353
28	17.07581318749262	29.887696657317424	34.15490516793977	18.88158498725018
29	18.410843998778414	27.92454279574244	33.38537246151165	20.279240743967488
30	19.881007919690184	29.9291968081419	31.25523435875241	18.93456091341551
31	24.08245321162454	28.8033529873551	27.91170866332042	19.202485137699938
32	23.855748098768487	28.926916641841522	28.567467254226365	18.64986800516363
33	22.319071520779115	28.99956532573403	28.42380928291861	20.25755387056824
34	19.894263611206352	28.995677614087946	30.125596506118228	20.984462268587478
35	19.669291574626264	28.724146717553534	30.2551556677215	21.3514060400987
36	23.529601879591482	28.870427723224907	28.448493909876287	19.151476487307324
37	19.562449764207944	30.91475513037418	30.38326279244491	19.139532312972964
38	19.72081546391173	31.86241997403196	28.2183226444683	20.19844191758801
39	19.608680744866817	30.866884753238043	28.547794496499186	20.976640005395954
40	22.303145954999973	27.700694916746766	28.32305665941585	21.673102468837417
41	18.546726546793995	27.913722778992494	29.80394691727886	23.735603756934648
42	20.37217110427873	28.95881461329916	28.06473461449826	22.604279667923848
43	20.77597787657874	28.798622157520708	28.4442314790354	21.981168486865155
44	20.622061967313243	30.41595704219151	28.003514866047258	20.95846612444799
45	19.542449127185314	31.643396604669377	26.96727577271782	21.84687849542749
46	21.36868996295901	30.906042909095	27.674464573110523	20.05080255483547
47	19.559358330850817	28.768363582540335	29.963905173560562	21.708372913048287
48	20.273057877253233	28.791455652920096	29.961563178593043	20.973923291233632
49	20.791997122156584	27.31595198348238	30.45389736066535	21.438153533695687
50	19.274056503907385	30.048310672190027	29.135963112642465	21.541669711260127
51	18.905098616724093	30.165176221069334	28.03958158854708	22.89014357365949
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1469.0
1	2400.5
2	3332.0
3	13216.5
4	23101.0
5	14679.0
6	6257.0
7	6787.0
8	7317.0
9	8383.0
10	9449.0
11	10234.0
12	11019.0
13	11848.5
14	12678.0
15	12311.5
16	11945.0
17	11770.5
18	11596.0
19	11955.0
20	12314.0
21	13022.0
22	13730.0
23	15496.0
24	17262.0
25	20107.5
26	26269.0
27	29585.0
28	34444.0
29	39303.0
30	45214.0
31	51125.0
32	59300.0
33	67475.0
34	75082.0
35	82689.0
36	84560.5
37	86432.0
38	97605.0
39	108778.0
40	126437.0
41	144096.0
42	158102.5
43	172109.0
44	175810.0
45	179511.0
46	179780.5
47	180050.0
48	181327.5
49	182605.0
50	172723.0
51	162841.0
52	145587.5
53	128334.0
54	116868.0
55	105402.0
56	91737.5
57	78073.0
58	71656.5
59	65240.0
60	52659.5
61	40079.0
62	33956.5
63	27834.0
64	21934.5
65	16035.0
66	12953.0
67	9871.0
68	7884.0
69	5897.0
70	4630.5
71	3364.0
72	2521.0
73	1678.0
74	1309.5
75	856.5
76	772.0
77	480.0
78	188.0
79	137.5
80	87.0
81	67.0
82	47.0
83	50.5
84	54.0
85	31.0
86	8.0
87	6.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2134932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.515760258208303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34925588326882	16.073945501101594
2	8.891658023979932	3.648382486358928
3	3.2227216146741107	1.983497520768
4	1.6609548732245252	1.3630300791510848
5	1.0072567096962226	1.033231858729971
6	0.6782087047157236	0.8348380314586661
7	0.4543623112947827	0.6525111774222421
8	0.36514113849718954	0.5992918446254059
9	0.30128445341180965	0.5562971654149812
>10	2.7237664861630178	12.90913289722941
>50	1.0398790680105368	15.53052073501868
>100	1.2990478086756	42.88956676428157
>500	0.00461637456260788	0.6481845208488668
>1k	0.0016157310969127578	0.4515698123038277
>5k	0.0	0.0
>10k+	2.3081872813039402E-4	0.8259996052868778
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17443	0.8170283643694506	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08904264866515654	0.0
2	0.0	0.0	0.0	0.32675513786855975	0.0
3	0.0	0.0	0.0	0.4520987085302951	0.0
4	0.0	0.0	0.0	0.6685458834286057	0.0
5	0.0	0.0	0.0	1.1702948852703505	0.0
6	0.0	0.0	0.0	1.5614548847457437	0.0
7	0.0	0.0	0.0	1.8439463177281525	0.0
8	0.0	0.0	0.0	2.4307097368909174	0.0
9	0.0	0.0	0.0	2.6345101389646133	0.0
10	4.6839899350424275E-5	0.0	0.0	3.0054821418199738	0.0
11	4.6839899350424275E-5	0.0	0.0	3.61777330612872	0.0
12	4.6839899350424275E-5	0.0	0.0	4.067014780798639	0.0
13	4.6839899350424275E-5	0.0	0.0	4.231703866914731	0.0
14	4.6839899350424275E-5	0.0	0.0	4.298638083086487	0.0
15	4.6839899350424275E-5	0.0	0.0	4.4022011005502755	0.0
16	4.6839899350424275E-5	0.0	0.0	4.634667521026431	0.0
17	4.6839899350424275E-5	0.0	0.0	4.948635366372325	0.0
18	4.6839899350424275E-5	0.0	0.0	5.256701384400065	0.0
19	4.6839899350424275E-5	0.0	0.0	5.49132244024634	0.0
20	3.278792954529699E-4	0.0	0.0	5.711376287394634	0.0
21	3.278792954529699E-4	0.0	0.0	5.993352481484187	0.0
22	4.215590941538185E-4	0.0	0.0	6.318515062774833	0.0
23	4.215590941538185E-4	0.0	0.0	6.650328909773239	0.0
24	4.215590941538185E-4	0.0	0.0	6.895395263174659	0.0
25	4.215590941538185E-4	0.0	0.0	7.111046159784012	0.0
26	4.215590941538185E-4	0.0	0.0	7.308804214841503	0.0
27	4.215590941538185E-4	0.0	0.0	7.517569646246344	0.0
28	4.215590941538185E-4	0.0	0.0	7.735656217621919	0.0
29	4.215590941538185E-4	0.0	0.0	7.981331489714895	0.0
30	4.215590941538185E-4	0.0	0.0	8.267335915148585	0.0
31	4.215590941538185E-4	0.0	0.0	8.521442369124637	0.0
32	4.215590941538185E-4	0.0	0.0	8.740184699091119	0.0
33	4.215590941538185E-4	0.0	0.0	8.97550835342765	0.0
34	4.215590941538185E-4	0.0	0.0	9.212518244140798	0.0
35	4.215590941538185E-4	0.0	0.0	9.48995096799336	0.0
36	4.6839899350424277E-4	0.0	0.0	9.740684949216181	0.0
37	4.6839899350424277E-4	0.0	0.0	9.996571319367549	0.0
38	4.6839899350424277E-4	0.0	0.0	10.276299198288283	0.0
39	4.6839899350424277E-4	0.0	0.0	10.626240086335303	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGA	25	3.8919334E-5	45.000004	36
CGACTGA	25	3.8919334E-5	45.000004	37
TCGCACG	40	6.8193913E-9	45.0	1
GTCGATC	20	7.034647E-4	45.0	23
CAACGCG	35	1.2125747E-7	45.0	1
CTATACG	20	7.034647E-4	45.0	1
CGATTCG	35	1.2125747E-7	45.0	10
TCGGCGA	20	7.034647E-4	45.0	27
ACCTCGA	20	7.034647E-4	45.0	16
ATATCCG	20	7.034647E-4	45.0	31
CGTACAA	20	7.034647E-4	45.0	32
TAGGCGT	20	7.034647E-4	45.0	17
ACGTACC	20	7.034647E-4	45.0	28
TCGCGGA	40	6.8193913E-9	45.0	39
CGCTTAC	20	7.034647E-4	45.0	26
CGCCACT	20	7.034647E-4	45.0	31
GTCGAAT	120	0.0	44.999996	11
CGTTAAT	30	2.16638E-6	44.999996	41
TTTAGCG	325	0.0	43.615383	1
ACGCTAG	100	0.0	42.750004	1
>>END_MODULE