FastQCFastQC Report
Thu 26 May 2016
SRR1547736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547736_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1189052
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76680.6448834870131837No Hit
GGGAATGGTTACATCAGTGCGGCAGAACTGCGCCACGTCATGACAAACTTA15870.13346767004302587No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCA253.8908867E-545.00000415
CTATACG351.2119744E-745.0000041
TCGATCT253.8908867E-545.00000436
CCGTACA253.8908867E-545.00000434
CGAGGTA351.2119744E-745.00000413
CGCTAGA253.8908867E-545.00000414
GTCACGA351.2119744E-745.00000421
TATAGCG351.2119744E-745.0000041
ACCGTAC253.8908867E-545.00000433
CACCGTA253.8908867E-545.00000432
ATTGCCG253.8908867E-545.00000415
CGTATTA207.033386E-445.024
ACGTTAG207.033386E-445.01
ATCACGT207.033386E-445.043
CCCCGTA207.033386E-445.021
TCGTGAC207.033386E-445.026
TACGATA207.033386E-445.036
AACGCTA406.8157533E-945.041
CGAATCG207.033386E-445.016
AATCCGT302.165565E-644.99999645