##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547736_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1189052
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50489213255602	31.0	31.0	33.0	30.0	34.0
2	31.918219724620958	31.0	31.0	34.0	30.0	34.0
3	32.05619434642051	33.0	31.0	34.0	30.0	34.0
4	35.733279116472616	37.0	35.0	37.0	33.0	37.0
5	35.61741034033835	37.0	35.0	37.0	33.0	37.0
6	35.666494821084356	37.0	35.0	37.0	33.0	37.0
7	36.06015296219173	37.0	35.0	37.0	35.0	37.0
8	36.076088346010096	37.0	35.0	37.0	35.0	37.0
9	37.857488991229985	39.0	38.0	39.0	35.0	39.0
10	37.241495746191084	39.0	37.0	39.0	34.0	39.0
11	36.91389274817249	39.0	37.0	39.0	33.0	39.0
12	36.42619246256682	39.0	35.0	39.0	32.0	39.0
13	36.23157776110717	39.0	35.0	39.0	32.0	39.0
14	37.26676797986968	39.0	35.0	41.0	32.0	41.0
15	37.44921332288243	39.0	35.0	41.0	32.0	41.0
16	37.51791343019481	39.0	35.0	41.0	32.0	41.0
17	37.433357834644745	39.0	35.0	41.0	32.0	41.0
18	37.321585599284134	39.0	35.0	41.0	32.0	41.0
19	37.30678977874811	39.0	35.0	41.0	32.0	41.0
20	37.18230573599809	39.0	35.0	41.0	32.0	41.0
21	37.08325455909414	39.0	35.0	41.0	32.0	41.0
22	37.01145198023299	39.0	35.0	41.0	32.0	41.0
23	36.9352425293427	39.0	35.0	41.0	31.0	41.0
24	36.861436673921745	38.0	35.0	41.0	31.0	41.0
25	36.7898367775337	38.0	35.0	41.0	31.0	41.0
26	36.65167292935885	38.0	35.0	41.0	31.0	41.0
27	36.62747550149194	38.0	35.0	41.0	31.0	41.0
28	36.662932319192095	38.0	35.0	41.0	31.0	41.0
29	36.67731015969024	38.0	35.0	41.0	31.0	41.0
30	36.66351093139745	38.0	35.0	41.0	31.0	41.0
31	36.557217009853225	38.0	35.0	41.0	31.0	41.0
32	36.398172661918906	38.0	35.0	41.0	30.0	41.0
33	36.31377769853631	38.0	35.0	41.0	30.0	41.0
34	36.119752542361475	38.0	35.0	41.0	30.0	41.0
35	36.04577848571803	38.0	35.0	41.0	30.0	41.0
36	35.93869149540979	38.0	35.0	41.0	29.0	41.0
37	35.741690859609164	38.0	35.0	41.0	29.0	41.0
38	35.82189761255185	38.0	35.0	41.0	29.0	41.0
39	35.716211738426914	38.0	35.0	40.0	29.0	41.0
40	35.58149433330082	38.0	35.0	40.0	28.0	41.0
41	35.54066853257889	38.0	34.0	40.0	28.0	41.0
42	35.52513514968227	38.0	34.0	40.0	28.0	41.0
43	35.48391912212418	38.0	34.0	40.0	28.0	41.0
44	35.330132744404786	38.0	34.0	40.0	27.0	41.0
45	35.29990530271174	38.0	34.0	40.0	27.0	41.0
46	35.10215028442827	37.0	34.0	40.0	26.0	41.0
47	35.14784719255339	37.0	34.0	40.0	27.0	41.0
48	34.98165176964506	37.0	34.0	40.0	26.0	41.0
49	34.95217114137986	37.0	34.0	40.0	26.0	41.0
50	34.83183662278857	37.0	34.0	40.0	26.0	41.0
51	34.142808724933815	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	0.0
13	8.0
14	16.0
15	30.0
16	97.0
17	237.0
18	452.0
19	912.0
20	1588.0
21	2420.0
22	3734.0
23	5429.0
24	7740.0
25	11116.0
26	15235.0
27	18672.0
28	20622.0
29	23336.0
30	27612.0
31	33859.0
32	42195.0
33	54908.0
34	90932.0
35	126314.0
36	86643.0
37	113099.0
38	173917.0
39	327728.0
40	197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.73956731917527	23.081917359375367	28.815140128438454	14.363375193010903
2	31.89439990849853	24.663177051970813	29.233120166317367	14.209302873213284
3	29.819385527294013	24.375300659685195	31.65832949273875	14.14698432028204
4	26.293299199698584	27.07829430504301	30.805969797788492	15.822436697469916
5	23.871537998338173	32.08076686301356	29.736714626441906	14.310980512206362
6	23.44514789933493	39.82744236585111	26.239811210947884	10.487598523866072
7	88.4593777227573	3.3305524064548906	6.418222247639297	1.791847623148525
8	88.42523287459252	3.0096244739506766	6.5685941405422135	1.9965485109145773
9	83.06020258155236	5.474697490101358	8.179709550129012	3.285390378217269
10	44.33086189670426	31.20755021647497	14.118558313681822	10.343029573138937
11	33.69474169338263	23.27232114323007	26.151841971587448	16.881095191799854
12	33.73452128250068	21.555323064088032	27.243720207358468	17.466435446052824
13	26.327023544807123	26.967702001258143	27.067781728637602	19.63749272529713
14	19.34734561650794	29.03859545251175	30.21718141847455	21.39687751250576
15	19.080073873976914	26.73339769833447	36.0380370244531	18.14849140323552
16	24.900256675065513	24.718431153557624	32.6794791144542	17.70183305692266
17	24.423238008093843	25.235229409647346	26.96778610186939	23.37374648038942
18	25.12446890464	26.078758540417073	30.172019390236926	18.624753164706
19	27.430171262484738	26.89739389025879	25.814262118057073	19.858172729199396
20	28.404729145571427	26.64626946508647	27.734867777019	17.214133612323092
21	26.512549493209715	26.450903745168418	29.309231219492503	17.72731554212936
22	26.456286184287986	22.02847310294251	30.01197592704104	21.50326478572846
23	23.530510019746824	26.171269212784637	29.926277404184177	20.371943363284366
24	23.082842466099045	24.632143926422057	33.306449171272575	18.978564436206323
25	22.993695818181205	27.11714878743739	29.282234923283422	20.606920471097983
26	21.435647894288895	30.3876533574646	26.607751385137068	21.568947363109437
27	20.385063058638313	30.033589784130548	29.572297931461367	20.009049225769772
28	18.68471690052243	28.304985820636947	32.58410902130437	20.426188257536257
29	21.022797993695818	25.757662406690372	31.63595873014805	21.58358086946576
30	22.013082691085	28.08909955157554	30.404893982769465	19.49292377456999
31	26.29868163881815	26.20583456400561	27.163572324843656	20.331911472332582
32	26.80185559588647	26.251753497744424	27.56641425269879	19.37997665367032
33	25.947561586877615	26.63432717828993	26.630794952617716	20.78731628221474
34	22.07170081712154	25.957653660226804	29.997342420684713	21.973303101966945
35	22.569155932625318	26.42550536057296	29.776662416782447	21.228676290019276
36	24.622304154906598	28.817831347998236	26.082458967311773	20.47740552978339
37	22.09524898826965	29.057013486374018	28.524908919038023	20.32282860631831
38	22.744421606456235	28.470327622341156	27.0988989547976	21.686351816405004
39	22.644762382132992	28.051086075293597	27.509394038275868	21.79475750429754
40	24.965182346945298	25.78020137050356	27.777422686308086	21.47719359624306
41	20.98570962413755	24.998654390220107	29.37701631215456	24.63861967348779
42	21.924272445612132	25.7573260042454	27.581720563945062	24.736680986197406
43	22.155128623474834	26.408264735268094	27.628228201962575	23.808378439294497
44	21.859430874343595	26.78368986385793	27.816613571147435	23.54026569065104
45	21.707208767993325	29.25052899284472	26.188089335033286	22.854172904128667
46	22.771417902665316	28.853490007165373	27.094357521790467	21.280734568378843
47	22.209794020782944	26.50800806020258	28.905968788581156	22.37622913043332
48	22.29296952530251	25.21420425683654	31.025472393133352	21.467353824727596
49	22.680084638855156	25.577518897407348	29.983970423497038	21.75842604024046
50	21.278127449430304	27.465325317984412	29.277609389665045	21.978937842920242
51	19.974736176382528	28.534328187497266	28.7566902036244	22.734245432495804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	880.0
1	1587.5
2	2295.0
3	7292.5
4	12290.0
5	8377.0
6	4464.0
7	4519.0
8	4574.0
9	4856.5
10	5139.0
11	4991.0
12	4843.0
13	4988.0
14	5133.0
15	4783.0
16	4433.0
17	4522.5
18	4612.0
19	4328.5
20	4045.0
21	4113.5
22	4182.0
23	4633.5
24	5085.0
25	6707.0
26	9871.0
27	11413.0
28	14033.5
29	16654.0
30	19191.5
31	21729.0
32	24892.0
33	28055.0
34	32929.5
35	37804.0
36	38084.5
37	38365.0
38	43032.0
39	47699.0
40	55641.5
41	63584.0
42	73653.0
43	83722.0
44	84453.0
45	85184.0
46	86709.0
47	88234.0
48	87220.0
49	86206.0
50	83786.5
51	81367.0
52	80007.5
53	78648.0
54	73757.5
55	68867.0
56	65390.0
57	61913.0
58	59143.5
59	56374.0
60	52600.5
61	48827.0
62	44616.0
63	40405.0
64	33642.0
65	26879.0
66	22292.5
67	17706.0
68	14740.0
69	11774.0
70	9585.0
71	7396.0
72	6020.0
73	4644.0
74	3506.5
75	1902.0
76	1435.0
77	1140.5
78	846.0
79	557.0
80	268.0
81	283.0
82	298.0
83	170.5
84	43.0
85	32.0
86	21.0
87	17.0
88	13.0
89	7.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1189052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.13070877576874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.02189803764446	15.691370445939734
2	6.564272575196297	2.511583739216917
3	1.915500590860702	1.099346518907071
4	0.9421705202574684	0.720975593606406
5	0.5774043228595116	0.5523076973247631
6	0.4115114741200681	0.472350370156699
7	0.3400363249630974	0.45535951342361747
8	0.2669087486989716	0.40849228328518944
9	0.1949914567928124	0.3357292296299568
>10	3.4431328942403994	17.325515589828278
>50	2.1328456923216237	29.907230965744485
>100	1.185355582356901	29.37003143464778
>500	0.0030891619793366747	0.3683485075338068
>1k	4.4130885419095355E-4	0.13398328706306475
>5k	4.4130885419095355E-4	0.6473748236922372
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7668	0.6448834870131837	No Hit
GGGAATGGTTACATCAGTGCGGCAGAACTGCGCCACGTCATGACAAACTTA	1587	0.13346767004302587	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.410061124324252E-5	0.0	0.0	0.046255336183783385	0.0
2	8.410061124324252E-5	0.0	0.0	0.17560207627589036	0.0
3	8.410061124324252E-5	0.0	0.0	0.2438917726054033	0.0
4	8.410061124324252E-5	0.0	0.0	0.35019494521686184	0.0
5	8.410061124324252E-5	0.0	0.0	0.5929934098761029	0.0
6	8.410061124324252E-5	0.0	0.0	0.7740620258828042	0.0
7	8.410061124324252E-5	0.0	0.0	0.9022313574175057	0.0
8	8.410061124324252E-5	0.0	0.0	1.1390586786784767	0.0
9	8.410061124324252E-5	0.0	0.0	1.2143287257411788	0.0
10	8.410061124324252E-5	0.0	0.0	1.3800069298903666	0.0
11	8.410061124324252E-5	0.0	0.0	1.630206248339013	0.0
12	8.410061124324252E-5	0.0	0.0	1.8222920444185788	0.0
13	8.410061124324252E-5	0.0	0.0	1.9000851098185783	0.0
14	8.410061124324252E-5	0.0	0.0	1.9363324732644156	0.0
15	8.410061124324252E-5	0.0	0.0	1.9955393035796585	0.0
16	8.410061124324252E-5	0.0	0.0	2.1224471259457114	0.0
17	8.410061124324252E-5	0.0	0.0	2.2734077231273315	0.0
18	8.410061124324252E-5	0.0	0.0	2.4226022074728437	0.0
19	8.410061124324252E-5	0.0	0.0	2.535297026538789	0.0
20	2.5230183372972753E-4	0.0	0.0	2.6563178061178148	0.0
21	2.5230183372972753E-4	0.0	0.0	2.808624013079327	0.0
22	2.5230183372972753E-4	0.0	0.0	2.9738817141722986	0.0
23	2.5230183372972753E-4	0.0	0.0	3.1375415036516485	0.0
24	2.5230183372972753E-4	0.0	0.0	3.262178609514134	0.0
25	3.3640244497297006E-4	0.0	0.0	3.3751257304138087	0.0
26	3.3640244497297006E-4	0.0	0.0	3.485633933587429	0.0
27	3.3640244497297006E-4	0.0	0.0	3.6074957192788877	0.0
28	3.3640244497297006E-4	0.0	0.0	3.7333102336987785	0.0
29	3.3640244497297006E-4	0.0	0.0	3.878804291149588	0.0
30	3.3640244497297006E-4	0.0	0.0	4.04692141302483	0.0
31	3.3640244497297006E-4	0.0	0.0	4.189471949082126	0.0
32	3.3640244497297006E-4	0.0	0.0	4.323360122181368	0.0
33	3.3640244497297006E-4	0.0	0.0	4.461285124620286	0.0
34	3.3640244497297006E-4	0.0	0.0	4.61493694136169	0.0
35	4.205030562162126E-4	0.0	0.0	4.777839825339851	0.0
36	4.205030562162126E-4	0.0	0.0	4.923922587069363	0.0
37	4.205030562162126E-4	0.0	0.0	5.088255181438659	0.0
38	4.205030562162126E-4	0.0	0.0	5.282275291576819	0.0
39	4.205030562162126E-4	0.0	0.0	5.560311912346979	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCA	25	3.8908867E-5	45.000004	15
CTATACG	35	1.2119744E-7	45.000004	1
TCGATCT	25	3.8908867E-5	45.000004	36
CCGTACA	25	3.8908867E-5	45.000004	34
CGAGGTA	35	1.2119744E-7	45.000004	13
CGCTAGA	25	3.8908867E-5	45.000004	14
GTCACGA	35	1.2119744E-7	45.000004	21
TATAGCG	35	1.2119744E-7	45.000004	1
ACCGTAC	25	3.8908867E-5	45.000004	33
CACCGTA	25	3.8908867E-5	45.000004	32
ATTGCCG	25	3.8908867E-5	45.000004	15
CGTATTA	20	7.033386E-4	45.0	24
ACGTTAG	20	7.033386E-4	45.0	1
ATCACGT	20	7.033386E-4	45.0	43
CCCCGTA	20	7.033386E-4	45.0	21
TCGTGAC	20	7.033386E-4	45.0	26
TACGATA	20	7.033386E-4	45.0	36
AACGCTA	40	6.8157533E-9	45.0	41
CGAATCG	20	7.033386E-4	45.0	16
AATCCGT	30	2.165565E-6	44.999996	45
>>END_MODULE