FastQCFastQC Report
Thu 26 May 2016
SRR1547735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547735_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1312710
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106030.8077183841061621No Hit
GGTTGAGGGGGTCATTCCCTTGACACAGTTGGGTTTCTCATAAGTATCCTA13150.10017444827875159No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAA302.165738E-645.00000432
ACGTTCG302.165738E-645.0000041
CCGCTCG302.165738E-645.00000419
TCCGTTC302.165738E-645.00000421
CGGTCTA1200.045.00000431
CACGTAC302.165738E-645.00000421
ACGCGAG453.8562575E-1045.01
CGAACTA207.0336537E-445.027
AGTTTCG207.0336537E-445.01
CGACGCA253.89111E-545.019
ACGTTAC207.0336537E-445.045
CTATCGC207.0336537E-445.029
TCTTCGA253.89111E-545.045
CGATTAC207.0336537E-445.010
TCGATCT207.0336537E-445.029
CGTTCAT253.89111E-545.017
ACCTCGT253.89111E-545.020
CCGTAGT207.0336537E-445.035
CGCTCAT207.0336537E-445.024
TATCTCG207.0336537E-445.016