##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547735_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1312710
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.559136442931035	31.0	31.0	33.0	30.0	34.0
2	31.966291869491357	31.0	31.0	34.0	30.0	34.0
3	32.08891910627633	33.0	31.0	34.0	30.0	34.0
4	35.76153377364383	37.0	35.0	37.0	35.0	37.0
5	35.63877246307258	37.0	35.0	37.0	33.0	37.0
6	35.69439099267927	37.0	35.0	37.0	33.0	37.0
7	36.121045775533055	37.0	35.0	37.0	35.0	37.0
8	36.163529644780645	37.0	35.0	37.0	35.0	37.0
9	37.9849113665623	39.0	38.0	39.0	35.0	39.0
10	37.317697739790205	39.0	37.0	39.0	34.0	39.0
11	36.92384456582185	39.0	37.0	39.0	33.0	39.0
12	36.589209345552334	39.0	35.0	39.0	32.0	39.0
13	36.44786891240259	39.0	35.0	39.0	32.0	39.0
14	37.504822847392035	40.0	36.0	41.0	32.0	41.0
15	37.698362166815215	40.0	36.0	41.0	33.0	41.0
16	37.76341309200052	40.0	36.0	41.0	33.0	41.0
17	37.7025702554258	40.0	36.0	41.0	33.0	41.0
18	37.61044480502167	39.0	36.0	41.0	33.0	41.0
19	37.58734373928743	39.0	36.0	41.0	33.0	41.0
20	37.47276550037708	39.0	35.0	41.0	32.0	41.0
21	37.37976476144769	39.0	35.0	41.0	32.0	41.0
22	37.31736103175873	39.0	35.0	41.0	32.0	41.0
23	37.260948724394574	39.0	35.0	41.0	32.0	41.0
24	37.183686419696656	39.0	35.0	41.0	32.0	41.0
25	37.09748383115844	39.0	35.0	41.0	32.0	41.0
26	36.978088077336196	39.0	35.0	41.0	32.0	41.0
27	36.95757402625104	39.0	35.0	41.0	31.0	41.0
28	37.01269815877079	39.0	35.0	41.0	32.0	41.0
29	36.98637703681697	39.0	35.0	41.0	32.0	41.0
30	36.99007930159746	39.0	35.0	41.0	32.0	41.0
31	36.90559300988033	39.0	35.0	41.0	31.0	41.0
32	36.75002094902911	39.0	35.0	41.0	31.0	41.0
33	36.65984033030905	39.0	35.0	41.0	31.0	41.0
34	36.502900107411385	39.0	35.0	41.0	30.0	41.0
35	36.43036771259456	39.0	35.0	41.0	30.0	41.0
36	36.330999230599296	39.0	35.0	41.0	30.0	41.0
37	36.10817240670064	39.0	35.0	41.0	30.0	41.0
38	36.21705784217382	39.0	35.0	41.0	30.0	41.0
39	36.13433355425037	39.0	35.0	41.0	30.0	41.0
40	36.043942683456365	38.0	35.0	41.0	29.0	41.0
41	35.9993738144754	38.0	35.0	41.0	29.0	41.0
42	35.98378088077336	38.0	35.0	41.0	29.0	41.0
43	35.945361123172674	38.0	35.0	41.0	29.0	41.0
44	35.80717218578361	38.0	35.0	40.0	29.0	41.0
45	35.77466919578582	38.0	35.0	40.0	29.0	41.0
46	35.60741519452126	38.0	35.0	40.0	28.0	41.0
47	35.66477211265245	38.0	35.0	40.0	29.0	41.0
48	35.52435572213208	38.0	34.0	40.0	28.0	41.0
49	35.49882076010695	38.0	34.0	40.0	28.0	41.0
50	35.40054696010543	38.0	34.0	40.0	28.0	41.0
51	34.73158351806568	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	9.0
15	19.0
16	64.0
17	147.0
18	369.0
19	765.0
20	1270.0
21	2126.0
22	3198.0
23	4828.0
24	7152.0
25	10810.0
26	14663.0
27	17597.0
28	19802.0
29	22645.0
30	27360.0
31	34289.0
32	43336.0
33	56296.0
34	92260.0
35	135725.0
36	96639.0
37	124565.0
38	190421.0
39	406048.0
40	302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.811519680660616	24.16939004045067	30.850378225198256	13.168712053690456
2	29.633963327772317	25.67741542305612	31.3767701929596	13.31185105621196
3	28.61690700916425	25.818040542084695	32.86041852351243	12.704633925238628
4	25.768067585376812	27.746722429173236	32.061841533926	14.423368451523947
5	23.424975813393665	32.75704458715177	30.28544004387869	13.53253955557587
6	21.985891781124543	41.25960798653168	26.545847902430847	10.208652329912928
7	90.06048556040558	2.8392409595417116	5.8063090857843696	1.2939643942683456
8	90.96053202916104	1.9500879859222526	5.6269853966222545	1.4623945882944442
9	86.98996731951459	4.664929801707917	6.6422134363263785	1.7028894424511127
10	43.562477622628	37.292852191268445	11.120049363530407	8.02462082257315
11	27.263066480791643	24.97360422332427	30.705030052334482	17.058299243549605
12	29.40390489902568	23.858734983355046	29.05531305467316	17.68204706294612
13	23.698379687821376	28.393323734869085	29.777483221732144	18.130813355577395
14	16.50646372770833	33.16642670506052	30.70960074959435	19.617508817636796
15	16.68670155632242	30.258015860319492	37.047938996427234	16.00734358693085
16	20.6666361953516	27.617676409869656	35.37262609411066	16.343061300668083
17	20.520754774474177	27.710918633970945	29.174760609731017	22.59356598182386
18	21.839400933945807	28.687828994979853	31.25808442077839	18.21468565029595
19	23.471673103731973	29.99641962047977	28.04000883668137	18.491898439106887
20	25.28410692384457	29.518705578536004	29.46240982395198	15.734777673667452
21	24.037830137654165	27.218197469357285	31.57658584150346	17.167386551485095
22	22.956936413983286	24.921498274561785	31.719115417723643	20.40244989373129
23	19.81816242734496	28.867838288730947	30.67067364459782	20.643325639326278
24	19.737718155571297	28.69879866840353	33.59751963495364	17.965963541071524
25	19.809173389400552	30.236305048335122	30.64401124391526	19.310510318349063
26	18.706492675457643	33.8361100319187	27.596879737337265	19.860517555286393
27	17.571207654394343	33.34590275079797	31.655125656085502	17.427763938722187
28	16.760975386795256	31.48760960151138	33.64116979378537	18.11024521790799
29	17.572883576722962	28.86776211044328	33.47380609578658	20.085548217047176
30	19.2840764525295	30.20613844642	32.11569958330477	18.394085517745733
31	22.33524540835371	30.099717378552764	28.406883470073357	19.158153743020165
32	22.720859900511154	30.403059320032604	29.75447737885748	17.12160340059876
33	22.346215081777395	30.28132641634481	28.45601846561693	18.916440036260866
34	18.817712975447737	30.1523565753289	30.369997943186235	20.65993250603713
35	19.496766231688646	29.60539646989815	30.02582443951825	20.87201285889496
36	22.37188716472031	29.4317099740232	29.616975569623143	18.579427291633337
37	20.119447555057857	30.623366927958195	30.74471894020766	18.512466576776287
38	19.833550441453177	31.605990660541934	28.485804176093733	20.074654721911163
39	19.23181814719169	30.16462127964288	29.403752542450352	21.199808030715086
40	21.22791781886327	29.365282507179803	28.64075081320322	20.76604886075371
41	18.243252508170123	28.512238041913296	30.901950925947087	22.3425585239695
42	19.836902286110416	29.13842356651507	29.530894104562318	21.493780042812197
43	21.049889160591448	29.07092960364437	28.577522834441726	21.301658401322456
44	19.54414912661593	30.677529690487614	29.074433804876932	20.703887378019516
45	18.93617021276596	32.047215302694426	27.76782381485629	21.248790669683327
46	20.790806804244653	31.16529926640309	28.10971196989434	19.934181959457913
47	18.985457564884854	29.732538032010115	31.42552429706485	19.856480106040173
48	19.930296866787028	29.638076955306197	30.291153415453525	20.140472762453246
49	20.60416999946675	28.18147191687425	30.38264353893853	20.831714544720466
50	19.24705380472458	29.17986455500453	30.66572205589963	20.907359584371264
51	17.72348805143558	30.895323414920277	28.536615093965917	22.84457343967822
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	863.0
1	1645.0
2	2427.0
3	8486.5
4	14546.0
5	9541.0
6	4536.0
7	4744.0
8	4952.0
9	5572.0
10	6192.0
11	6791.0
12	7390.0
13	7829.5
14	8269.0
15	7828.0
16	7387.0
17	7288.0
18	7189.0
19	6821.0
20	6453.0
21	7098.0
22	7743.0
23	8386.0
24	9029.0
25	11011.0
26	16369.0
27	19745.0
28	23671.0
29	27597.0
30	33501.0
31	39405.0
32	43731.0
33	48057.0
34	52434.5
35	56812.0
36	58944.5
37	61077.0
38	68185.0
39	75293.0
40	83141.5
41	90990.0
42	98567.5
43	106145.0
44	107991.5
45	109838.0
46	111114.5
47	112391.0
48	107959.0
49	103527.0
50	94413.5
51	85300.0
52	78196.0
53	71092.0
54	62447.0
55	53802.0
56	50778.0
57	47754.0
58	42921.0
59	38088.0
60	32192.5
61	26297.0
62	21902.5
63	17508.0
64	13600.5
65	9693.0
66	7250.0
67	4807.0
68	3995.5
69	3184.0
70	2371.0
71	1558.0
72	1433.0
73	1308.0
74	996.5
75	564.5
76	444.0
77	341.0
78	238.0
79	155.5
80	73.0
81	47.5
82	22.0
83	14.5
84	7.0
85	4.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1312710.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.220683096929474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10979749943496	15.996541450983381
2	8.475663494252112	3.4276741110697175
3	2.81436702917779	1.7072527144635288
4	1.4018789689776179	1.1338780148778653
5	0.8390184990175801	0.848276359054796
6	0.5649004828801868	0.6853604187373612
7	0.4126214104004356	0.58404407451007
8	0.3195795810948632	0.5169693946854962
9	0.2430248251641438	0.4422715176897759
>10	3.1125860622508843	15.18789561760542
>50	1.4095493768768204	20.85484362793349
>100	1.2905712813992976	36.95321584793868
>500	0.00568366682941558	0.7486385543709312
>1k	3.7891112196103866E-4	0.10075321860585146
>5k	0.0	0.0
>10k+	3.7891112196103866E-4	0.8123850774736449
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10603	0.8077183841061621	No Hit
GGTTGAGGGGGTCATTCCCTTGACACAGTTGGGTTTCTCATAAGTATCCTA	1315	0.10017444827875159	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.617828766444988E-5	0.0	0.0	0.06673217999405809	0.0
2	7.617828766444988E-5	0.0	0.0	0.2501694966900534	0.0
3	7.617828766444988E-5	0.0	0.0	0.34790623976354257	0.0
4	7.617828766444988E-5	0.0	0.0	0.5003389933801068	0.0
5	7.617828766444988E-5	0.0	0.0	0.8941045623176482	0.0
6	7.617828766444988E-5	0.0	0.0	1.2040740148242948	0.0
7	7.617828766444988E-5	0.0	0.0	1.4255242970648505	0.0
8	7.617828766444988E-5	0.0	0.0	1.8566172269579724	0.0
9	7.617828766444988E-5	0.0	0.0	1.9976994157125336	0.0
10	7.617828766444988E-5	0.0	0.0	2.246650059799956	0.0
11	7.617828766444988E-5	0.0	0.0	2.6948069261299143	0.0
12	7.617828766444988E-5	0.0	0.0	3.0529972347281578	0.0
13	7.617828766444988E-5	0.0	0.0	3.1919464314281143	0.0
14	7.617828766444988E-5	0.0	0.0	3.2481660077244783	0.0
15	7.617828766444988E-5	0.0	0.0	3.3288388143611307	0.0
16	7.617828766444988E-5	0.0	0.0	3.5096098909888704	0.0
17	7.617828766444988E-5	0.0	0.0	3.774481797198163	0.0
18	7.617828766444988E-5	0.0	0.0	4.01360544217687	0.0
19	7.617828766444988E-5	0.0	0.0	4.213649625583716	0.0
20	7.617828766444988E-5	0.0	0.0	4.389164400362609	0.0
21	7.617828766444988E-5	0.0	0.0	4.6144997752740515	0.0
22	1.5235657532889976E-4	0.0	0.0	4.88531358792117	0.0
23	1.5235657532889976E-4	0.0	0.0	5.1463765797472405	0.0
24	1.5235657532889976E-4	0.0	0.0	5.344135414524152	0.0
25	1.5235657532889976E-4	0.0	0.0	5.508985229030022	0.0
26	1.5235657532889976E-4	0.0	0.0	5.672692369220925	0.0
27	1.5235657532889976E-4	0.0	0.0	5.848130965712152	0.0
28	1.5235657532889976E-4	0.0	0.0	6.014123454532989	0.0
29	1.5235657532889976E-4	0.0	0.0	6.219881009514668	0.0
30	1.5235657532889976E-4	0.0	0.0	6.445292562713775	0.0
31	1.5235657532889976E-4	0.0	0.0	6.645641459271278	0.0
32	1.5235657532889976E-4	0.0	0.0	6.834335077816121	0.0
33	1.5235657532889976E-4	0.0	0.0	7.024552262114252	0.0
34	1.5235657532889976E-4	0.0	0.0	7.22703415072636	0.0
35	1.5235657532889976E-4	0.0	0.0	7.45076978159685	0.0
36	1.5235657532889976E-4	0.0	0.0	7.661021855550731	0.0
37	1.5235657532889976E-4	0.0	0.0	7.886509587037502	0.0
38	1.5235657532889976E-4	0.0	0.0	8.128604185235124	0.0
39	1.5235657532889976E-4	0.0	0.0	8.461427124041105	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAA	30	2.165738E-6	45.000004	32
ACGTTCG	30	2.165738E-6	45.000004	1
CCGCTCG	30	2.165738E-6	45.000004	19
TCCGTTC	30	2.165738E-6	45.000004	21
CGGTCTA	120	0.0	45.000004	31
CACGTAC	30	2.165738E-6	45.000004	21
ACGCGAG	45	3.8562575E-10	45.0	1
CGAACTA	20	7.0336537E-4	45.0	27
AGTTTCG	20	7.0336537E-4	45.0	1
CGACGCA	25	3.89111E-5	45.0	19
ACGTTAC	20	7.0336537E-4	45.0	45
CTATCGC	20	7.0336537E-4	45.0	29
TCTTCGA	25	3.89111E-5	45.0	45
CGATTAC	20	7.0336537E-4	45.0	10
TCGATCT	20	7.0336537E-4	45.0	29
CGTTCAT	25	3.89111E-5	45.0	17
ACCTCGT	25	3.89111E-5	45.0	20
CCGTAGT	20	7.0336537E-4	45.0	35
CGCTCAT	20	7.0336537E-4	45.0	24
TATCTCG	20	7.0336537E-4	45.0	16
>>END_MODULE