FastQCFastQC Report
Thu 26 May 2016
SRR1547734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547734_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences878693
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74350.8461430784130521No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28340.32252447669436307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGA302.1649157E-645.00000413
AACGTAG453.8380676E-1045.0000041
AGCCGTC302.1649157E-645.00000431
AGCCGTA453.8380676E-1045.00000421
CGAACCG302.1649157E-645.00000436
CGAGTGA302.1649157E-645.00000412
CGAGTAT302.1649157E-645.00000419
ACGTTCG302.1649157E-645.00000433
CCTAGAT302.1649157E-645.00000430
TAGACGC302.1649157E-645.00000412
GCGCGAC302.1649157E-645.0000049
GCAACCG600.045.0000041
GAGTATC302.1649157E-645.00000421
GAGTACT302.1649157E-645.00000422
TGGAACG453.8380676E-1045.0000041
ATAGCGT302.1649157E-645.00000437
TCCTCGA302.1649157E-645.00000436
TTACTAA750.045.00000428
TACGGTG302.1649157E-645.00000415
TAACGGA302.1649157E-645.00000444
Produced by FastQC (version 0.11.5)