##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547734_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	878693
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.594158596916103	31.0	31.0	33.0	30.0	34.0
2	32.00916816225917	31.0	31.0	34.0	30.0	34.0
3	32.10918830581329	33.0	31.0	34.0	30.0	34.0
4	35.77229817467534	37.0	35.0	37.0	35.0	37.0
5	35.66680285378397	37.0	35.0	37.0	33.0	37.0
6	35.72895197753937	37.0	35.0	37.0	33.0	37.0
7	36.11294843591561	37.0	35.0	37.0	35.0	37.0
8	36.14379766312011	37.0	35.0	37.0	35.0	37.0
9	37.97866490344182	39.0	38.0	39.0	35.0	39.0
10	37.380360376149575	39.0	37.0	39.0	35.0	39.0
11	36.96603478120345	39.0	37.0	39.0	33.0	39.0
12	36.077738186146924	37.0	35.0	39.0	32.0	39.0
13	35.66447211938641	37.0	35.0	39.0	31.0	39.0
14	36.65969684520077	38.0	35.0	41.0	31.0	41.0
15	36.97946836949879	38.0	35.0	41.0	32.0	41.0
16	37.146737256356886	38.0	35.0	41.0	33.0	41.0
17	37.115119842766475	38.0	35.0	41.0	32.0	41.0
18	37.03243453629425	38.0	35.0	41.0	32.0	41.0
19	36.9653940568549	38.0	35.0	41.0	32.0	41.0
20	36.81589701977824	38.0	35.0	41.0	32.0	41.0
21	36.650561686504844	38.0	35.0	41.0	32.0	41.0
22	36.542192779503196	38.0	35.0	40.0	32.0	41.0
23	36.53546574287038	38.0	35.0	40.0	32.0	41.0
24	36.45677500560492	37.0	35.0	40.0	32.0	41.0
25	36.35364456072826	37.0	35.0	40.0	31.0	41.0
26	36.227094104539354	37.0	35.0	40.0	31.0	41.0
27	36.19162665458812	37.0	35.0	40.0	31.0	41.0
28	36.29952782143479	37.0	35.0	40.0	31.0	41.0
29	36.31486082169768	37.0	35.0	40.0	31.0	41.0
30	36.33713139856582	37.0	35.0	40.0	31.0	41.0
31	36.159169357215774	37.0	35.0	40.0	31.0	41.0
32	35.918821476898074	37.0	35.0	40.0	30.0	41.0
33	35.75880199341522	37.0	35.0	40.0	30.0	41.0
34	35.62860066029888	37.0	35.0	40.0	30.0	41.0
35	35.54715469452926	37.0	35.0	40.0	30.0	41.0
36	35.360716427694314	37.0	35.0	40.0	29.0	41.0
37	35.131941417537185	37.0	34.0	40.0	27.0	41.0
38	35.240311462592736	37.0	34.0	40.0	29.0	41.0
39	35.19889881904146	36.0	34.0	40.0	28.0	41.0
40	35.05674450576026	36.0	34.0	40.0	27.0	41.0
41	35.07368102397538	36.0	34.0	40.0	27.0	41.0
42	35.02564263058884	36.0	34.0	40.0	27.0	41.0
43	34.984751215726085	36.0	34.0	40.0	27.0	41.0
44	34.835584214281894	36.0	34.0	40.0	27.0	41.0
45	34.79859518625959	36.0	34.0	40.0	27.0	41.0
46	34.61698795825163	35.0	34.0	40.0	26.0	41.0
47	34.68334332924013	35.0	34.0	40.0	27.0	41.0
48	34.58328335379934	35.0	34.0	40.0	26.0	41.0
49	34.602995585488905	36.0	34.0	40.0	26.0	41.0
50	34.473726318520804	35.0	34.0	40.0	26.0	41.0
51	33.75621974910464	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	1.0
14	11.0
15	24.0
16	48.0
17	139.0
18	308.0
19	658.0
20	1118.0
21	1916.0
22	2942.0
23	4135.0
24	6326.0
25	9109.0
26	12341.0
27	14532.0
28	15947.0
29	17830.0
30	20863.0
31	25668.0
32	32374.0
33	43050.0
34	80552.0
35	144718.0
36	55508.0
37	68480.0
38	103880.0
39	216049.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.017886793225845	24.905968296094315	30.043143623540875	14.033001287138966
2	30.39172953466114	26.278347500207694	29.49995049465513	13.829972470476037
3	28.694322135262258	24.295288570638437	33.81510948647593	13.195279807623367
4	24.979714189142282	27.929322300280074	32.987402881324876	14.103560629252765
5	23.877736592871457	32.04270433473352	30.151144939131186	13.928414133263836
6	22.032268380424107	40.668811518926404	26.653222456534877	10.64569764411461
7	87.8776774140684	4.225366538711473	6.5135377202276565	1.3834183269924762
8	89.00628547171765	3.2254723777246435	6.148563832874508	1.6196783176831955
9	85.6525544188926	5.281025341046304	7.068338998945024	1.9980812411160669
10	53.60006282057556	27.170695567166238	11.380311439831658	7.848930172426548
11	42.522815135661716	21.199668143481283	23.642159434523776	12.635357286333223
12	40.97938642961763	21.395868636713846	23.293801134184523	14.330943799484006
13	21.49487932645418	39.00179015879266	23.15279625534743	16.35053425940573
14	13.851936910843717	41.26230663041586	27.578460281349688	17.30729617739074
15	13.806187143860257	28.202455237494778	44.511678140146785	13.479679478498177
16	16.820891938367552	22.135717480394177	43.78525833254618	17.25813224869209
17	16.869942061675694	23.745608534493844	27.34618347932668	32.038265924503776
18	20.98855914409242	25.82938523466102	33.516028920225835	19.66602670102072
19	27.728797202208277	25.706020191352387	26.005442173774	20.559740432665333
20	29.861624025683604	25.3310314296347	26.980071538068472	17.827273006613233
21	23.130490398808227	28.476157201662012	28.793901851955116	19.599450547574637
22	22.42091378900253	24.922356272327196	27.828490724291647	24.828239214378627
23	19.595808775078442	31.089697994635213	26.37929288158663	22.935200348699716
24	20.025879345801094	25.245222165193077	37.57671905887494	17.152179430130886
25	16.665661385717197	28.40593927571973	34.33383445640286	20.594564882160206
26	16.721539832455704	36.141860695373694	27.30066132312423	19.835938149046367
27	17.511235437177717	37.00427794462912	28.984412075662373	16.500074542530783
28	14.338568760647915	29.984875263601733	39.199128705930285	16.477427269820062
29	15.168892889780617	25.15759201450336	38.5158411413315	21.157673954384524
30	17.06898768967091	33.74045315030392	32.07331798477966	17.117241175245507
31	27.127790934945423	28.73563349201598	25.27947758773542	18.857097985303174
32	28.249343058383303	27.896660153204817	27.287346092435016	16.566650695976865
33	25.996565353314526	30.74748518538329	25.012262530827034	18.243686930475146
34	19.136490218995714	29.825889133064678	27.86513606003462	23.17248458790499
35	20.159145458083767	27.49458570854667	30.725293134234594	21.620975699134963
36	27.92499769544084	26.537596179780653	28.3625794219369	17.174826702841607
37	19.63108844613534	32.712449057862074	30.571200635489298	17.085261860513285
38	19.78324625324203	32.892489185642766	25.487969063142646	21.836295497972557
39	19.47563028270397	32.37968209602216	29.287931052142213	18.85675656913165
40	23.460526031275997	28.92136388932198	26.3619944622297	21.256115617172323
41	17.506227999995446	26.050736719195438	29.344719942004772	27.098315338804333
42	21.802609102382746	25.757801644032675	26.20095983466353	26.238629418921057
43	22.08393602771389	26.75291597861824	26.825182401589633	24.337965592078234
44	18.556310338195477	30.98101384670186	27.9520833783813	22.510592436721357
45	17.836832659415748	38.419903197134836	23.166794318379687	20.576469825069736
46	21.744909769396138	34.38459166056859	25.622828450892403	18.247670119142864
47	20.084033900349723	28.79014627406842	29.165362646567118	21.960457179014742
48	22.325772482539406	25.59062152537917	32.305253370631156	19.778352621450267
49	20.253831542984866	25.746648715763072	32.106890574978976	21.89262916627309
50	19.04225935565664	31.411994860548564	28.795950348984228	20.749795434810565
51	17.140457474908757	34.82922932127603	25.52916661450586	22.501146589309347
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	690.0
1	1249.5
2	1809.0
3	6103.0
4	10397.0
5	6916.0
6	3435.0
7	3632.0
8	3829.0
9	4116.5
10	4404.0
11	4654.5
12	4905.0
13	4836.0
14	4767.0
15	4541.5
16	4316.0
17	4255.0
18	4194.0
19	3911.5
20	3629.0
21	3506.0
22	3383.0
23	3703.5
24	4024.0
25	4989.5
26	7656.5
27	9358.0
28	11111.0
29	12864.0
30	14981.5
31	17099.0
32	19633.5
33	22168.0
34	26672.0
35	31176.0
36	30229.0
37	29282.0
38	33608.0
39	37934.0
40	51098.5
41	64263.0
42	74950.0
43	85637.0
44	92547.5
45	99458.0
46	91461.5
47	83465.0
48	74673.5
49	65882.0
50	63207.5
51	60533.0
52	53201.0
53	45869.0
54	40693.5
55	35518.0
56	34176.0
57	32834.0
58	29697.0
59	26560.0
60	24368.5
61	22177.0
62	18158.5
63	14140.0
64	12173.0
65	10206.0
66	7745.0
67	5284.0
68	4282.5
69	3281.0
70	2623.5
71	1966.0
72	1387.5
73	809.0
74	605.0
75	424.5
76	448.0
77	361.5
78	275.0
79	148.5
80	22.0
81	15.5
82	9.0
83	15.0
84	21.0
85	15.5
86	10.0
87	5.5
88	1.0
89	1.5
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	878693.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.969957995397635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.78005794372237	14.04774106161551
2	7.847668684139775	2.6634921586329883
3	2.4444423463375466	1.2444625181855824
4	1.1162681194262567	0.7577209239306035
5	0.633250784510514	0.537311960684801
6	0.4101966808548299	0.4176612266374796
7	0.2700367092033264	0.32077581286770984
8	0.21735701748038955	0.2950831565317696
9	0.15271452639176095	0.23324031883397042
>10	1.5208522460287672	6.016928611417067
>50	0.6760037968560365	8.543090916471156
>100	1.9203888955462531	62.79741987003381
>500	0.009416968314412449	0.9528977054728058
>1k	6.726405938866035E-4	0.3234921055713862
>5k	6.726405938866035E-4	0.848681653113358
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7435	0.8461430784130521	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2834	0.32252447669436307	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06099968931128392	0.0
2	0.0	0.0	0.0	0.23967415240590287	0.0
3	0.0	0.0	0.0	0.3262800545810653	0.0
4	0.0	0.0	0.0	0.5232771855471706	0.0
5	0.0	0.0	0.0	0.8578650336351832	0.0
6	0.0	0.0	0.0	1.1446546177106225	0.0
7	0.0	0.0	0.0	1.300568002704016	0.0
8	0.0	0.0	0.0	1.6154675182344687	0.0
9	0.0	0.0	0.0	1.7124297109456887	0.0
10	0.0	0.0	0.0	1.8930388656789117	0.0
11	0.0	0.0	0.0	2.199516782311911	0.0
12	0.0	0.0	0.0	2.4090325062336904	0.0
13	0.0	0.0	0.0	2.4938175221607546	0.0
14	0.0	0.0	0.0	2.535584100476503	0.0
15	0.0	0.0	0.0	2.596469984397281	0.0
16	0.0	0.0	0.0	2.7416856626830985	0.0
17	0.0	0.0	0.0	2.909890029851154	0.0
18	0.0	0.0	0.0	3.09129582231792	0.0
19	0.0	0.0	0.0	3.21705077882719	0.0
20	1.1380539050612672E-4	0.0	0.0	3.3251658998080105	0.0
21	1.1380539050612672E-4	0.0	0.0	3.478575566210269	0.0
22	2.2761078101225343E-4	0.0	0.0	3.6384721398713773	0.0
23	2.2761078101225343E-4	0.0	0.0	3.809066420240061	0.0
24	2.2761078101225343E-4	0.0	0.0	3.9355042090923678	0.0
25	2.2761078101225343E-4	0.0	0.0	4.048057740302927	0.0
26	2.2761078101225343E-4	0.0	0.0	4.155262418159698	0.0
27	2.2761078101225343E-4	0.0	0.0	4.255183551024078	0.0
28	2.2761078101225343E-4	0.0	0.0	4.364664336690971	0.0
29	2.2761078101225343E-4	0.0	0.0	4.475738397824951	0.0
30	2.2761078101225343E-4	0.0	0.0	4.629603285789234	0.0
31	2.2761078101225343E-4	0.0	0.0	4.775387991027583	0.0
32	2.2761078101225343E-4	0.0	0.0	4.902394806832421	0.0
33	2.2761078101225343E-4	0.0	0.0	5.025190823188531	0.0
34	2.2761078101225343E-4	0.0	0.0	5.15891215703323	0.0
35	2.2761078101225343E-4	0.0	0.0	5.327116524201285	0.0
36	2.2761078101225343E-4	0.0	0.0	5.466869543742809	0.0
37	2.2761078101225343E-4	0.0	0.0	5.60992291960901	0.0
38	2.2761078101225343E-4	0.0	0.0	5.745806555873325	0.0
39	2.2761078101225343E-4	0.0	0.0	5.886583823929405	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGGA	30	2.1649157E-6	45.000004	13
AACGTAG	45	3.8380676E-10	45.000004	1
AGCCGTC	30	2.1649157E-6	45.000004	31
AGCCGTA	45	3.8380676E-10	45.000004	21
CGAACCG	30	2.1649157E-6	45.000004	36
CGAGTGA	30	2.1649157E-6	45.000004	12
CGAGTAT	30	2.1649157E-6	45.000004	19
ACGTTCG	30	2.1649157E-6	45.000004	33
CCTAGAT	30	2.1649157E-6	45.000004	30
TAGACGC	30	2.1649157E-6	45.000004	12
GCGCGAC	30	2.1649157E-6	45.000004	9
GCAACCG	60	0.0	45.000004	1
GAGTATC	30	2.1649157E-6	45.000004	21
GAGTACT	30	2.1649157E-6	45.000004	22
TGGAACG	45	3.8380676E-10	45.000004	1
ATAGCGT	30	2.1649157E-6	45.000004	37
TCCTCGA	30	2.1649157E-6	45.000004	36
TTACTAA	75	0.0	45.000004	28
TACGGTG	30	2.1649157E-6	45.000004	15
TAACGGA	30	2.1649157E-6	45.000004	44
>>END_MODULE