FastQCFastQC Report
Thu 26 May 2016
SRR1547733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547733_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1219928
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81050.6643834718114512No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15400.12623695824671619No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA253.890947E-545.044
CTCCGTA207.0334575E-445.040
TTAATCG850.045.020
ACGCATA207.0334575E-445.024
GCGGTAT207.0334575E-445.037
CGGCATA253.890947E-545.06
GACGTAA207.0334575E-445.042
CGTGAAT207.0334575E-445.017
ATTACGC351.2120108E-745.035
TTCACGT207.0334575E-445.015
ATTAACG207.0334575E-445.013
TGACGTA207.0334575E-445.019
CGCGAAT207.0334575E-445.016
GCGCATC253.890947E-545.034
TAGCCCG406.8157533E-945.013
GCCGATA351.2120108E-745.09
ACGATAC406.8157533E-945.041
GTAGCAC253.890947E-545.031
CCGTACA351.2120108E-745.042
TACGCGG453.8562575E-1045.02