##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547733_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1219928 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.570029542727113 31.0 31.0 33.0 30.0 34.0 2 31.97624859827793 31.0 31.0 34.0 30.0 34.0 3 32.10719567056417 33.0 31.0 34.0 30.0 34.0 4 35.78487336957591 37.0 35.0 37.0 35.0 37.0 5 35.66128820717288 37.0 35.0 37.0 33.0 37.0 6 35.71101573207599 37.0 35.0 37.0 33.0 37.0 7 36.08955446550944 37.0 35.0 37.0 35.0 37.0 8 36.11471332734391 37.0 35.0 37.0 35.0 37.0 9 37.93779632896368 39.0 38.0 39.0 35.0 39.0 10 37.32999816382606 39.0 37.0 39.0 34.0 39.0 11 37.002641139477085 39.0 37.0 39.0 33.0 39.0 12 36.33961184594501 38.0 35.0 39.0 32.0 39.0 13 36.034689752182096 38.0 35.0 39.0 32.0 39.0 14 36.96261746594881 39.0 35.0 41.0 31.0 41.0 15 37.234211363293575 39.0 35.0 41.0 32.0 41.0 16 37.3940962089566 39.0 35.0 41.0 33.0 41.0 17 37.351260074365044 39.0 35.0 41.0 32.0 41.0 18 37.25637250722993 39.0 35.0 41.0 32.0 41.0 19 37.20322920696959 39.0 35.0 41.0 32.0 41.0 20 37.04203362821412 39.0 35.0 41.0 32.0 41.0 21 36.93622410502915 38.0 35.0 41.0 32.0 41.0 22 36.875209028729564 38.0 35.0 41.0 32.0 41.0 23 36.84152425389039 38.0 35.0 41.0 32.0 41.0 24 36.73204648143169 38.0 35.0 41.0 31.0 41.0 25 36.63152825412647 38.0 35.0 40.0 31.0 41.0 26 36.48045048560243 38.0 35.0 40.0 31.0 41.0 27 36.43792256592192 38.0 35.0 40.0 31.0 41.0 28 36.50334855827557 38.0 35.0 41.0 31.0 41.0 29 36.488237830429334 38.0 35.0 41.0 31.0 41.0 30 36.530810834737785 38.0 35.0 41.0 31.0 41.0 31 36.3566218662085 38.0 35.0 41.0 31.0 41.0 32 36.164284285629975 38.0 35.0 41.0 30.0 41.0 33 36.05926415329429 38.0 35.0 41.0 30.0 41.0 34 35.87889285269294 38.0 35.0 41.0 30.0 41.0 35 35.789402325383136 38.0 35.0 41.0 29.0 41.0 36 35.621377655074724 38.0 35.0 40.0 29.0 41.0 37 35.39289121980969 38.0 34.0 40.0 27.0 41.0 38 35.47723964037222 38.0 34.0 40.0 29.0 41.0 39 35.39003121495695 38.0 34.0 40.0 28.0 41.0 40 35.23876409099553 37.0 34.0 40.0 27.0 41.0 41 35.17357171898669 37.0 34.0 40.0 27.0 41.0 42 35.185349463247015 37.0 34.0 40.0 27.0 41.0 43 35.13280373923707 37.0 34.0 40.0 27.0 41.0 44 34.993175007049594 37.0 34.0 40.0 27.0 41.0 45 34.95932055006525 37.0 34.0 40.0 27.0 41.0 46 34.77758605425894 36.0 34.0 40.0 26.0 41.0 47 34.80180141778859 36.0 34.0 40.0 26.0 41.0 48 34.64637995029215 36.0 34.0 40.0 26.0 41.0 49 34.63026998314655 36.0 34.0 40.0 26.0 41.0 50 34.506368408627395 36.0 34.0 40.0 26.0 41.0 51 33.82142060842935 35.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 2.0 12 5.0 13 6.0 14 14.0 15 29.0 16 80.0 17 217.0 18 497.0 19 941.0 20 1671.0 21 2703.0 22 4032.0 23 6076.0 24 8767.0 25 12548.0 26 16568.0 27 19423.0 28 21819.0 29 24382.0 30 28321.0 31 34518.0 32 44122.0 33 58140.0 34 101851.0 35 154537.0 36 88336.0 37 112071.0 38 165409.0 39 312626.0 40 214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.07717504639618 23.95485635217816 28.25420844508856 13.713760156337095 2 30.93854719294909 25.4100241981494 30.097431979592237 13.55399662930927 3 29.162048907804394 25.18624049943931 31.509154638634413 14.142555954121882 4 25.91538189139031 27.862627958371313 31.115278934494494 15.106711215743879 5 24.103553652346697 31.347505754437964 29.568876195972223 14.980064397243115 6 22.79765691089966 40.6575633971841 26.075063446367324 10.469716245548916 7 88.0892970732699 3.546110918021391 6.543500927923615 1.8210910807850957 8 88.63285374218806 2.9374684407604383 6.468660445534491 1.9610173715170074 9 84.4325238866556 4.732082549134047 7.941616226531402 2.893777337678945 10 47.47173603688086 27.77131109376947 13.525880215881594 11.231072653468074 11 38.20356611209842 22.38386199841302 23.837718291571306 15.574853597917254 12 35.961056718101396 21.637014643487156 25.943744220970416 16.45818441744103 13 25.030247686748723 29.186066718691595 26.76461233777731 19.019073256782367 14 17.823920755979042 33.02990012525329 29.794053419546074 19.35212569922159 15 17.50349200936449 26.668049261923656 38.83761992511033 16.990838803601523 16 22.49304877009135 23.125545114137882 36.540763061426574 17.84064305434419 17 22.08097527067171 23.888295046920803 27.400961368211895 26.62976831419559 18 24.822366565895692 25.729879140408286 30.880920841229976 18.566833452466046 19 28.209205789194115 26.526155641972316 25.77988209140212 19.484756477431457 20 29.12253837931419 26.963558505092106 26.5629610927858 17.350942022807903 21 25.904397636581834 25.67258067689241 29.461574781462513 18.96144690506325 22 25.595035116826566 21.875143451088917 28.906869913634246 23.622951518450268 23 22.502885416188494 26.533451154494365 28.67931550058692 22.28434792873022 24 22.730275885134205 24.81293978005259 34.36784793856687 18.088936396246336 25 21.894078994825925 26.742971716363588 30.15104170082169 21.211907587988797 26 20.237833708218847 31.38603261831846 26.688788190778475 21.687345482684222 27 19.879369930028655 31.45398744843958 30.065134991573274 18.601507629958487 28 18.126561567567922 27.43604540595839 34.28685955236703 20.15053347410667 29 18.218780124728674 26.293436989723983 33.79437147110321 21.69341141444413 30 21.401508941511302 28.31921228138054 31.00486258205402 19.274416195054133 31 26.508285734895832 26.689116078981712 27.287512049891472 19.51508613623099 32 27.763933609196606 26.039077716061932 26.767973191860506 19.429015482880956 33 25.810949498658935 27.847381156920736 25.697746096490942 20.643923247929386 34 21.284616797056877 27.33718711268206 29.18049261923655 22.197703471024518 35 22.701749611452477 27.68384691555567 28.9339206904014 20.68048278259045 36 27.987881252008318 26.09768773239076 27.232836692001495 18.681594323599427 37 22.048760254703556 29.814054599943603 28.618574210937037 19.5186109344158 38 22.320333659035615 29.881189709556626 25.615446157478143 22.18303047392961 39 21.53840226636326 28.845882707831937 27.796722429520432 21.81899259628437 40 24.96844076043832 25.99497675272639 26.89371831780236 22.142864169032926 41 19.930930349987868 25.000983664609716 29.31082818002374 25.757257805378682 42 22.519361798401217 25.680531965820936 27.31259549743919 24.487510738338656 43 22.385747355581643 25.62929943406496 27.489163294882978 24.49578991547042 44 21.36478546274862 28.009357929320416 28.30486717248887 22.320989435442094 45 20.40522063597196 31.08683463286358 25.249522922664287 23.258421808500174 46 23.311375753323148 29.26795679745034 26.455741650326903 20.964925798899607 47 21.504383865277298 27.34644995442354 29.308041130296214 21.84112505000295 48 23.079558793633723 26.35180108990039 30.48106117738096 20.08757893908493 49 22.65912414503151 25.098284488920658 29.59920585477176 22.643385511276076 50 20.433828881704493 29.13696546025667 29.108193270422518 21.321012387616317 51 19.74788676053013 30.777308169006695 26.278354132375025 23.19645093808815 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 950.0 1 1719.5 2 2489.0 3 7566.5 4 12644.0 5 8714.0 6 4784.0 7 5068.5 8 5353.0 9 5645.0 10 5937.0 11 6033.5 12 6130.0 13 5981.5 14 5833.0 15 5611.0 16 5389.0 17 4944.5 18 4500.0 19 4369.5 20 4239.0 21 4616.5 22 4994.0 23 5622.5 24 6251.0 25 8097.5 26 10296.0 27 10648.0 28 13140.0 29 15632.0 30 19516.5 31 23401.0 32 25396.5 33 27392.0 34 31042.0 35 34692.0 36 37763.5 37 40835.0 38 44324.0 39 47813.0 40 56770.0 41 65727.0 42 78643.5 43 91560.0 44 94873.5 45 98187.0 46 97178.5 47 96170.0 48 91964.5 49 87759.0 50 85668.5 51 83578.0 52 76824.5 53 70071.0 54 67651.5 55 65232.0 56 62147.5 57 59063.0 58 57770.5 59 56478.0 60 52947.0 61 49416.0 62 42739.5 63 36063.0 64 31769.0 65 27475.0 66 23105.0 67 18735.0 68 15555.5 69 12376.0 70 11123.0 71 9870.0 72 7680.5 73 5491.0 74 4302.0 75 2526.0 76 1939.0 77 1471.5 78 1004.0 79 626.0 80 248.0 81 287.0 82 326.0 83 251.0 84 176.0 85 94.0 86 12.0 87 7.0 88 2.0 89 3.0 90 4.0 91 2.5 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1219928.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.66586045101968 #Duplication Level Percentage of deduplicated Percentage of total 1 82.55925130821694 14.584802125516246 2 7.421135754946173 2.6220149726990334 3 2.128023946279755 1.127801222142191 4 1.055750169193311 0.746029406404378 5 0.6147845122434236 0.5430348700370261 6 0.40670930227504254 0.431092186687349 7 0.2793611923484056 0.345460908762018 8 0.23517654696343943 0.33236768480070383 9 0.1957589033497798 0.31124245217596724 >10 2.091177282105249 8.58339289594579 >50 1.0820579277283071 14.390769680876256 >100 1.9247472841214308 54.51968589153999 >500 0.0041994486199013045 0.4813079479362964 >1k 0.001399816206633768 0.31290374556708017 >5k 4.66605402211256E-4 0.6680940089096904 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8105 0.6643834718114512 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1540 0.12623695824671619 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.197205080955598E-5 0.0 0.0 0.04352715897987422 0.0 2 8.197205080955598E-5 0.0 0.0 0.1813221763907378 0.0 3 8.197205080955598E-5 0.0 0.0 0.25534293827176685 0.0 4 8.197205080955598E-5 0.0 0.0 0.3886294928881049 0.0 5 8.197205080955598E-5 0.0 0.0 0.6288076017601039 0.0 6 8.197205080955598E-5 0.0 0.0 0.852345384317763 0.0 7 8.197205080955598E-5 0.0 0.0 0.9787462866660983 0.0 8 8.197205080955598E-5 0.0 0.0 1.2313841472611498 0.0 9 8.197205080955598E-5 0.0 0.0 1.312208589359372 0.0 10 8.197205080955598E-5 0.0 0.0 1.4759067748260553 0.0 11 8.197205080955598E-5 0.0 0.0 1.775432648484173 0.0 12 8.197205080955598E-5 0.0 0.0 1.9823301047274922 0.0 13 8.197205080955598E-5 0.0 0.0 2.0697942829412885 0.0 14 8.197205080955598E-5 0.0 0.0 2.111026224498495 0.0 15 8.197205080955598E-5 0.0 0.0 2.1816041602455227 0.0 16 8.197205080955598E-5 0.0 0.0 2.3217763671298632 0.0 17 8.197205080955598E-5 0.0 0.0 2.4632601268271572 0.0 18 8.197205080955598E-5 0.0 0.0 2.628515781259222 0.0 19 8.197205080955598E-5 0.0 0.0 2.7438504567482673 0.0 20 2.4591615242866794E-4 0.0 0.0 2.865333036048029 0.0 21 2.4591615242866794E-4 0.0 0.0 3.017964994655422 0.0 22 2.4591615242866794E-4 0.0 0.0 3.1746955558032934 0.0 23 2.4591615242866794E-4 0.0 0.0 3.3334754182214033 0.0 24 2.4591615242866794E-4 0.0 0.0 3.452088975742831 0.0 25 2.4591615242866794E-4 0.0 0.0 3.566112098418923 0.0 26 2.4591615242866794E-4 0.0 0.0 3.676856339062633 0.0 27 3.278882032382239E-4 0.0 0.0 3.823832226164167 0.0 28 3.278882032382239E-4 0.0 0.0 3.942035923431547 0.0 29 3.278882032382239E-4 0.0 0.0 4.091716888209796 0.0 30 3.278882032382239E-4 0.0 0.0 4.241971657343712 0.0 31 3.278882032382239E-4 0.0 0.0 4.371733413775239 0.0 32 3.278882032382239E-4 0.0 0.0 4.493625853329049 0.0 33 4.098602540477799E-4 0.0 0.0 4.61707576184824 0.0 34 4.098602540477799E-4 0.0 0.0 4.767904335337823 0.0 35 4.098602540477799E-4 0.0 0.0 4.928159694670505 0.0 36 4.098602540477799E-4 0.0 0.0 5.061774137490081 0.0 37 4.098602540477799E-4 0.0 0.0 5.215635676859618 0.0 38 4.098602540477799E-4 0.0 0.0 5.382612744358683 0.0 39 4.098602540477799E-4 0.0 0.0 5.687958633624279 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTTA 25 3.890947E-5 45.0 44 CTCCGTA 20 7.0334575E-4 45.0 40 TTAATCG 85 0.0 45.0 20 ACGCATA 20 7.0334575E-4 45.0 24 GCGGTAT 20 7.0334575E-4 45.0 37 CGGCATA 25 3.890947E-5 45.0 6 GACGTAA 20 7.0334575E-4 45.0 42 CGTGAAT 20 7.0334575E-4 45.0 17 ATTACGC 35 1.2120108E-7 45.0 35 TTCACGT 20 7.0334575E-4 45.0 15 ATTAACG 20 7.0334575E-4 45.0 13 TGACGTA 20 7.0334575E-4 45.0 19 CGCGAAT 20 7.0334575E-4 45.0 16 GCGCATC 25 3.890947E-5 45.0 34 TAGCCCG 40 6.8157533E-9 45.0 13 GCCGATA 35 1.2120108E-7 45.0 9 ACGATAC 40 6.8157533E-9 45.0 41 GTAGCAC 25 3.890947E-5 45.0 31 CCGTACA 35 1.2120108E-7 45.0 42 TACGCGG 45 3.8562575E-10 45.0 2 >>END_MODULE