FastQCFastQC Report
Thu 26 May 2016
SRR1547732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547732_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1543245
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89720.5813723679649051No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17830.11553577040586556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGC253.8914295E-545.044
CGCCGAT253.8914295E-545.023
CGTTAGG2200.043.9772722
GCGTTAG1150.041.0869561
CGTTTTT41700.040.953241
ATTCCGG501.0822987E-940.52
AATGCGG3000.039.752
TACGGGA6300.039.6428534
ACGTATG403.459245E-739.3751
TAGGGAT24550.039.317725
AGGGATC17100.038.947376
GTAGGGA18750.038.6399964
GCGATAA1050.038.571439
ATAGGGA20100.038.3955234
TAGGGAC17000.038.255
ACGGGAT7700.037.987015
CGAATAT3500.037.9285714
AAGGGAT25150.037.8429455
ACCGTTC1850.037.702745
GGGCGAT21100.037.6421787
Produced by FastQC (version 0.11.5)