##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547732_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1543245
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.579964296012623	31.0	31.0	33.0	30.0	34.0
2	32.00185258983505	31.0	31.0	34.0	30.0	34.0
3	32.160312199294346	33.0	31.0	34.0	30.0	34.0
4	35.827108463011385	37.0	35.0	37.0	35.0	37.0
5	35.72001529245195	37.0	35.0	37.0	33.0	37.0
6	35.77675417707493	37.0	35.0	37.0	33.0	37.0
7	36.121230912784426	37.0	35.0	37.0	35.0	37.0
8	36.145872495941994	37.0	35.0	37.0	35.0	37.0
9	37.96320480545863	39.0	38.0	39.0	35.0	39.0
10	37.33788218980136	39.0	37.0	39.0	34.0	39.0
11	37.00080738962381	39.0	37.0	39.0	33.0	39.0
12	36.634331554613816	39.0	35.0	39.0	33.0	39.0
13	36.50159566368269	39.0	35.0	39.0	32.0	39.0
14	37.586104604259205	40.0	36.0	41.0	33.0	41.0
15	37.751946061707635	40.0	36.0	41.0	33.0	41.0
16	37.81504621754809	40.0	36.0	41.0	33.0	41.0
17	37.740617335549445	40.0	36.0	41.0	33.0	41.0
18	37.65475086587029	39.0	36.0	41.0	33.0	41.0
19	37.63671743631115	39.0	36.0	41.0	33.0	41.0
20	37.52808594876381	39.0	35.0	41.0	33.0	41.0
21	37.43665458174172	39.0	35.0	41.0	32.0	41.0
22	37.35216248878176	39.0	35.0	41.0	32.0	41.0
23	37.25756052992234	39.0	35.0	41.0	32.0	41.0
24	37.171130961059326	39.0	35.0	41.0	32.0	41.0
25	37.06960916769534	39.0	35.0	41.0	32.0	41.0
26	36.94686326539208	39.0	35.0	41.0	31.0	41.0
27	36.91658809845488	39.0	35.0	41.0	31.0	41.0
28	36.98325411713629	39.0	35.0	41.0	31.0	41.0
29	36.95208473055153	39.0	35.0	41.0	31.0	41.0
30	36.973271904331455	39.0	35.0	41.0	31.0	41.0
31	36.88796399793941	39.0	35.0	41.0	31.0	41.0
32	36.72710165916624	39.0	35.0	41.0	31.0	41.0
33	36.658020599451156	39.0	35.0	41.0	31.0	41.0
34	36.51303908323047	39.0	35.0	41.0	30.0	41.0
35	36.433848157615934	39.0	35.0	41.0	30.0	41.0
36	36.315427556868805	39.0	35.0	41.0	30.0	41.0
37	36.12386691678898	39.0	35.0	41.0	30.0	41.0
38	36.185318598148704	39.0	35.0	41.0	30.0	41.0
39	36.079593324455935	38.0	35.0	41.0	30.0	41.0
40	35.93827940476075	38.0	35.0	41.0	29.0	41.0
41	35.88636541832308	38.0	35.0	41.0	29.0	41.0
42	35.883424861250155	38.0	35.0	40.0	29.0	41.0
43	35.842167640264506	38.0	35.0	40.0	29.0	41.0
44	35.69706754274273	38.0	35.0	40.0	29.0	41.0
45	35.643821298627245	38.0	35.0	40.0	29.0	41.0
46	35.45893944253829	38.0	34.0	40.0	28.0	41.0
47	35.46914391428451	38.0	34.0	40.0	28.0	41.0
48	35.32757792832635	37.0	34.0	40.0	28.0	41.0
49	35.28885303370495	37.0	34.0	40.0	28.0	41.0
50	35.175112182446725	37.0	34.0	40.0	27.0	41.0
51	34.48386808316243	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	2.0
13	2.0
14	12.0
15	31.0
16	74.0
17	183.0
18	498.0
19	967.0
20	1564.0
21	2567.0
22	3875.0
23	5792.0
24	8592.0
25	12360.0
26	17009.0
27	20559.0
28	23328.0
29	26840.0
30	32403.0
31	39609.0
32	50529.0
33	65994.0
34	111041.0
35	156407.0
36	116070.0
37	153298.0
38	237771.0
39	455544.0
40	318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.58701955943483	23.188800222906927	28.976150902805454	14.248029314852795
2	30.15224413492349	25.674277253449713	30.20395335802157	13.969525253605228
3	28.940900505104505	26.133828394065752	31.165563471775382	13.759707629054363
4	25.860313819257474	28.6356346529553	30.980822876471333	14.523228651315895
5	23.50871054174807	32.54486487887536	29.28718382369617	14.6592407556804
6	23.10747807379904	40.043285414823956	26.105770632660402	10.7434658787166
7	91.07018004270223	2.615592469115403	5.197748899235053	1.1164785889473154
8	91.71622133880233	2.081911815687075	5.116556347177538	1.085310498333058
9	87.02098500238135	4.7629507952399	6.188777543423112	2.027286658955642
10	44.21067296508331	34.62839665769207	11.495582360545473	9.66534801667914
11	30.427897061062893	23.885773159802884	27.507427530949396	18.17890224818483
12	31.032337703993857	22.090141228385644	28.074997813049773	18.802523254570726
13	24.36871656801091	28.25092580892859	27.62179692790192	19.75856069515858
14	18.945274405554528	29.586682607103864	29.984480753218058	21.48356223412355
15	19.06340211696782	26.404621430816235	34.90962225699743	19.622354195218517
16	24.019063726109593	24.843106570894445	32.47397529232235	18.663854410673615
17	22.976585052924197	25.099903126204847	27.540345181743664	24.383166639127293
18	24.125268508888738	24.832933202440312	30.360182602243974	20.681615686426976
19	25.859989826631548	26.594869900761058	26.472271091109967	21.072869181497428
20	27.519933646310207	26.35122744606333	27.596071913403254	18.532766994223213
21	26.22713827033297	25.3340201977003	29.02429620701833	19.414545324948403
22	26.04479522046078	22.870639464245794	28.497354600209302	22.587210715084126
23	23.248868455753946	26.997268742163428	28.335811876921678	21.418050925160944
24	22.411023525104568	25.618615320315307	32.374315160586946	19.596045993993176
25	22.448995460863312	28.386030733940498	28.82990063146163	20.335073173734568
26	22.13180668007996	29.61026927027141	26.85970147319447	21.39822257645416
27	20.364491704168813	28.864891835061833	29.146959815194606	21.623656645574744
28	19.479862238335453	28.16040226924435	31.306305868478436	21.053429623941756
29	20.281225599305362	26.25532562878869	31.25530942915739	22.20813934274856
30	21.876889281999944	27.344329643057325	30.522826900459744	20.255954174482987
31	25.50528269976575	26.772417859769508	27.164837728293307	20.55746171217143
32	25.289698006473373	26.6454127504058	27.57915949832982	20.48572974479101
33	24.493388930467944	26.859312682043356	26.82477506811945	21.822523319369253
34	21.02368710088158	26.16933798586744	29.396434137159037	23.41054077609194
35	21.60564265557316	27.422411865905932	29.165686588973234	21.806258889547674
36	24.945099449536528	27.197431386461645	27.31471671704752	20.542752446954307
37	21.712106632453047	28.772100346996105	28.18236896928226	21.333424051268594
38	21.84902591617015	28.849664181643224	27.923952450842222	21.37735745134441
39	21.962812126396003	28.30820770519263	27.513259398216093	22.215720770195272
40	24.43915256488762	26.46676321646919	27.808675874537098	21.285408344106088
41	20.334101195856782	25.8417814410544	28.938114168521523	24.88600319456729
42	21.93028326675285	26.47103991913144	27.661259229739933	23.93741758437578
43	22.549562771951308	26.59849861817145	27.852803670188464	22.999134939688773
44	21.827707201384097	27.907040035768787	28.006246577827888	22.259006185019228
45	21.597348444349407	28.437739957038577	26.557157159103056	23.407754439508956
46	22.139906495728155	28.8556256459603	27.43310362256155	21.571364235749993
47	21.595339690068656	27.134706414082014	29.547285103791037	21.722668792058293
48	22.395471879060032	26.7406017839034	29.575537260771945	21.288389076264625
49	22.69529465509365	26.468642373699574	28.788559172393235	22.04750379881354
50	21.212574801797512	28.27205012813908	29.365460442120337	21.14991462794307
51	20.89758917087047	28.246454710690784	28.006052182252333	22.849903936186415
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1138.0
1	2046.5
2	2955.0
3	8450.5
4	13946.0
5	9444.0
6	4942.0
7	5072.5
8	5203.0
9	5414.0
10	5625.0
11	5710.0
12	5795.0
13	5608.5
14	5422.0
15	5133.0
16	4844.0
17	4767.0
18	4690.0
19	4847.5
20	5005.0
21	5609.5
22	6214.0
23	6495.5
24	6777.0
25	8727.5
26	13108.0
27	15538.0
28	19282.5
29	23027.0
30	26155.0
31	29283.0
32	34062.0
33	38841.0
34	45290.0
35	51739.0
36	55653.0
37	59567.0
38	61832.5
39	64098.0
40	74125.0
41	84152.0
42	94406.0
43	104660.0
44	107807.5
45	110955.0
46	110741.5
47	110528.0
48	112200.5
49	113873.0
50	111440.5
51	109008.0
52	105066.0
53	101124.0
54	96316.0
55	91508.0
56	87899.5
57	84291.0
58	77782.0
59	71273.0
60	65045.5
61	58818.0
62	50437.0
63	42056.0
64	37120.0
65	32184.0
66	27085.5
67	21987.0
68	18820.0
69	15653.0
70	13247.0
71	10841.0
72	8739.5
73	6638.0
74	5139.5
75	3128.0
76	2615.0
77	1851.5
78	1088.0
79	922.5
80	757.0
81	475.0
82	193.0
83	117.0
84	41.0
85	28.5
86	16.0
87	12.5
88	9.0
89	8.5
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1543245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.490011325380753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.90174459591474	14.77384162496144
2	5.3668765998203485	1.9846721822519837
3	1.7746107700266067	0.9843771970780386
4	1.0069696026656998	0.7447551743041178
5	0.6559630939662267	0.6064382518233665
6	0.5147121278848669	0.5710219844341208
7	0.3777991762731102	0.4889857733606529
8	0.32925007855016825	0.4870270145020091
9	0.3051894625649004	0.5078660957290687
>10	6.459166609561323	33.22811703797176
>50	3.0680026528663378	38.88359395630675
>100	0.2351324387457271	5.510764093620616
>500	0.0035252239692010077	0.43938802714641945
>1k	7.050447938402015E-4	0.20434378654562005
>5k	3.5252239692010076E-4	0.5848077999640521
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8972	0.5813723679649051	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1783	0.11553577040586556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.479852518556677E-5	0.0	0.0	0.03278805374389679	0.0
2	6.479852518556677E-5	0.0	0.0	0.12020126421922638	0.0
3	6.479852518556677E-5	0.0	0.0	0.1661434185757932	0.0
4	6.479852518556677E-5	0.0	0.0	0.2299699658835765	0.0
5	6.479852518556677E-5	0.0	0.0	0.38373686614892644	0.0
6	6.479852518556677E-5	0.0	0.0	0.503614137742225	0.0
7	6.479852518556677E-5	0.0	0.0	0.5761236874248742	0.0
8	6.479852518556677E-5	0.0	0.0	0.7144685386960593	0.0
9	6.479852518556677E-5	0.0	0.0	0.7723984202119559	0.0
10	6.479852518556677E-5	0.0	0.0	0.8867030186392958	0.0
11	6.479852518556677E-5	0.0	0.0	1.0883560290167795	0.0
12	6.479852518556677E-5	0.0	0.0	1.2368094502169131	0.0
13	6.479852518556677E-5	0.0	0.0	1.301283982776552	0.0
14	6.479852518556677E-5	0.0	0.0	1.3345904247219333	0.0
15	6.479852518556677E-5	0.0	0.0	1.3861700507696444	0.0
16	6.479852518556677E-5	0.0	0.0	1.4868669589080152	0.0
17	6.479852518556677E-5	0.0	0.0	1.6144552549983961	0.0
18	6.479852518556677E-5	0.0	0.0	1.7450890817724989	0.0
19	6.479852518556677E-5	0.0	0.0	1.8302343438663335	0.0
20	1.2959705037113355E-4	0.0	0.0	1.9225722422557663	0.0
21	1.2959705037113355E-4	0.0	0.0	2.037978415611261	0.0
22	1.9439557555670032E-4	0.0	0.0	2.161484404614951	0.0
23	1.9439557555670032E-4	0.0	0.0	2.2925718210653527	0.0
24	1.9439557555670032E-4	0.0	0.0	2.397091842189672	0.0
25	1.9439557555670032E-4	0.0	0.0	2.485930620219084	0.0
26	1.9439557555670032E-4	0.0	0.0	2.57353822626997	0.0
27	1.9439557555670032E-4	0.0	0.0	2.6789654267468874	0.0
28	1.9439557555670032E-4	0.0	0.0	2.7759688189496807	0.0
29	1.9439557555670032E-4	0.0	0.0	2.88735748374367	0.0
30	1.9439557555670032E-4	0.0	0.0	3.0286182686482057	0.0
31	1.9439557555670032E-4	0.0	0.0	3.142210083298504	0.0
32	1.9439557555670032E-4	0.0	0.0	3.2499700306821016	0.0
33	1.9439557555670032E-4	0.0	0.0	3.3591555456197817	0.0
34	1.9439557555670032E-4	0.0	0.0	3.480523183292348	0.0
35	1.9439557555670032E-4	0.0	0.0	3.6182848478368634	0.0
36	1.9439557555670032E-4	0.0	0.0	3.730969483134564	0.0
37	2.591941007422671E-4	0.0	0.0	3.8501987694760067	0.0
38	2.591941007422671E-4	0.0	0.0	3.9752599230841508	0.0
39	2.591941007422671E-4	0.0	0.0	4.17814410544016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGC	25	3.8914295E-5	45.0	44
CGCCGAT	25	3.8914295E-5	45.0	23
CGTTAGG	220	0.0	43.977272	2
GCGTTAG	115	0.0	41.086956	1
CGTTTTT	4170	0.0	40.95324	1
ATTCCGG	50	1.0822987E-9	40.5	2
AATGCGG	300	0.0	39.75	2
TACGGGA	630	0.0	39.642853	4
ACGTATG	40	3.459245E-7	39.375	1
TAGGGAT	2455	0.0	39.31772	5
AGGGATC	1710	0.0	38.94737	6
GTAGGGA	1875	0.0	38.639996	4
GCGATAA	105	0.0	38.57143	9
ATAGGGA	2010	0.0	38.395523	4
TAGGGAC	1700	0.0	38.25	5
ACGGGAT	770	0.0	37.98701	5
CGAATAT	350	0.0	37.92857	14
AAGGGAT	2515	0.0	37.842945	5
ACCGTTC	185	0.0	37.7027	45
GGGCGAT	2110	0.0	37.642178	7
>>END_MODULE