FastQCFastQC Report
Thu 26 May 2016
SRR1547731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547731_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1723160
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126210.7324334362450382No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC207.0342666E-445.00000410
CCCGCGA207.0342666E-445.00000418
GTCGAAT351.2123928E-745.035
TCCGATC253.8916194E-545.023
ATACTCG253.8916194E-545.044
TTGAGCG1750.045.01
GCCGATT502.1827873E-1145.09
CCCGTCG253.8916194E-545.029
TCGTGCC351.2123928E-745.040
GTTCGCA253.8916194E-545.09
CGCCGAC502.1827873E-1145.013
GCGCTAA351.2123928E-745.08
TCGCGAG253.8916194E-545.01
CGGACTA351.2123928E-745.034
TTTACGC302.1661363E-644.99999627
AATCGCG302.1661363E-644.9999961
CGACGGT2250.044.028
CGGTCTA2300.043.0434831
CGTTTTT58900.042.6315771
TCGTGCG850.042.352941
Produced by FastQC (version 0.11.5)