FastQCFastQC Report
Thu 26 May 2016
SRR1547730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547730_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences737037
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69880.9481206506593293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATA351.2111377E-745.09
TACGCGG253.8894388E-545.02
TGCGACG207.0316374E-445.01
TATTACG207.0316374E-445.01
TTCGGTA207.0316374E-445.025
ACCCGTT207.0316374E-445.025
TACGAAT900.042.512
CGTTTTT32250.041.6511651
CGTTAGG1050.040.7142872
TACGGGT451.9266736E-840.04
ACACGAC3850.039.1558426
GGCGATA1900.039.0789458
AATCGTT750.039.022
CATACGA2900.038.79310618
CTAGGGA5300.038.6320764
GCGAGAC4050.038.33333621
TAACGGG1000.038.253
ATTGGGC1950.038.0769234
TAAGGGA7900.037.879754
CGATTGA301.1395244E-437.50000410
Produced by FastQC (version 0.11.5)