##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547730_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	737037
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539313494437863	31.0	31.0	33.0	30.0	34.0
2	31.959806631146062	31.0	31.0	34.0	30.0	34.0
3	32.09358281877301	33.0	31.0	34.0	30.0	34.0
4	35.782029938795475	37.0	35.0	37.0	35.0	37.0
5	35.68234701921342	37.0	35.0	37.0	33.0	37.0
6	35.731747524208416	37.0	35.0	37.0	33.0	37.0
7	36.078921411001076	37.0	35.0	37.0	35.0	37.0
8	36.09232236644836	37.0	35.0	37.0	35.0	37.0
9	37.89222793428281	39.0	38.0	39.0	35.0	39.0
10	37.271980918189996	39.0	37.0	39.0	34.0	39.0
11	37.013111960457884	39.0	37.0	39.0	33.0	39.0
12	36.63276606194805	39.0	35.0	39.0	32.0	39.0
13	36.51733766418782	39.0	35.0	39.0	32.0	39.0
14	37.60195621115358	40.0	36.0	41.0	32.0	41.0
15	37.72000048844224	40.0	36.0	41.0	33.0	41.0
16	37.7766095867643	40.0	36.0	41.0	33.0	41.0
17	37.6915080246989	40.0	36.0	41.0	33.0	41.0
18	37.564483194195134	39.0	36.0	41.0	32.0	41.0
19	37.51501213643277	39.0	36.0	41.0	32.0	41.0
20	37.383545195153026	39.0	35.0	41.0	32.0	41.0
21	37.2923516729825	39.0	35.0	41.0	32.0	41.0
22	37.21068955832611	39.0	35.0	41.0	32.0	41.0
23	37.102400557909576	39.0	35.0	41.0	32.0	41.0
24	36.98768582852693	39.0	35.0	41.0	31.0	41.0
25	36.87234426494192	39.0	35.0	41.0	31.0	41.0
26	36.71735204609809	38.0	35.0	41.0	31.0	41.0
27	36.69162606490583	39.0	35.0	41.0	31.0	41.0
28	36.695118426890375	39.0	35.0	41.0	31.0	41.0
29	36.661574656360536	39.0	35.0	41.0	31.0	41.0
30	36.65902661603149	39.0	35.0	41.0	31.0	41.0
31	36.554181133375934	39.0	35.0	41.0	30.0	41.0
32	36.37884258185139	39.0	35.0	41.0	30.0	41.0
33	36.23840458484445	39.0	35.0	41.0	30.0	41.0
34	36.02378849365771	38.0	35.0	41.0	29.0	41.0
35	35.891500698065364	38.0	35.0	41.0	29.0	41.0
36	35.74985923366127	38.0	35.0	41.0	28.0	41.0
37	35.54390213788453	38.0	35.0	41.0	27.0	41.0
38	35.58753088379552	38.0	35.0	40.0	27.0	41.0
39	35.467637309931526	38.0	34.0	40.0	27.0	41.0
40	35.31325157353023	38.0	34.0	40.0	26.0	41.0
41	35.23091920758388	38.0	34.0	40.0	26.0	41.0
42	35.19610141688952	38.0	34.0	40.0	26.0	41.0
43	35.141752720691095	38.0	34.0	40.0	26.0	41.0
44	34.975354018862014	37.0	34.0	40.0	26.0	41.0
45	34.90087471863692	37.0	34.0	40.0	26.0	41.0
46	34.72016872965672	37.0	34.0	40.0	25.0	41.0
47	34.72837320243082	37.0	34.0	40.0	25.0	41.0
48	34.56010078191461	36.0	34.0	40.0	24.0	41.0
49	34.51856012656081	36.0	34.0	40.0	24.0	41.0
50	34.38478936606982	36.0	33.0	40.0	24.0	41.0
51	33.71039445780877	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	7.0
14	5.0
15	15.0
16	50.0
17	152.0
18	288.0
19	549.0
20	948.0
21	1578.0
22	2437.0
23	3629.0
24	5250.0
25	7461.0
26	10425.0
27	12105.0
28	13604.0
29	14646.0
30	17108.0
31	20688.0
32	25392.0
33	32918.0
34	52820.0
35	70319.0
36	60913.0
37	78031.0
38	113662.0
39	191921.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.503695201190716	23.09232779358431	27.526569222440667	14.87740778278431
2	33.15613734452951	24.750996218643024	28.12260442827158	13.970262008555881
3	29.609639678876366	25.445940977182964	31.54020761508581	13.404211728854861
4	25.859760093455282	27.591694853854015	30.70809199538151	15.840453057309198
5	23.805317779161697	32.23257448404897	29.32091604627719	14.641191690512144
6	23.187438351127557	40.395801024914626	26.19719227121569	10.219568352742128
7	88.49487881883813	3.0705378427405954	6.925839544012037	1.508743794409236
8	88.80897431200876	2.6377237506393847	7.1510656859832	1.402236251368656
9	83.79728561795406	5.153472620777519	8.457241631017167	2.5920001302512627
10	41.92584632793198	32.96130316388458	14.548387665748125	10.564462842435319
11	32.65087098748096	25.444855549992738	25.026016332965646	16.87825712956066
12	29.92156431766655	22.55694083200708	28.841835620192747	18.67965923013363
13	24.730508780427577	25.763971143918145	29.636232645036813	19.869287430617458
14	21.520357865344618	26.400574190983626	31.143483977059493	20.93558396661226
15	20.835453308314236	25.180554029173567	33.88052431560424	20.103468346907956
16	25.16929272207501	23.93963939395173	31.666388525949174	19.224679358024087
17	24.473533893142406	24.037056484274196	28.567900933060347	22.921508689523048
18	25.340654539731382	24.660634405057007	29.776659787771848	20.22205126743976
19	27.124147091665684	25.975358089213973	26.900277733682298	20.000217085438045
20	28.8916295925442	25.5588254049661	27.41761946822208	18.131925534267616
21	28.2661521741785	25.118820357729664	28.4544737916821	18.160553676409734
22	27.33363453937862	22.324795091698245	28.879554215052977	21.46201615387016
23	25.390719868880396	25.453946002710854	28.558132088348348	20.597202040060402
24	23.30222227649358	25.67971485827713	30.941323162880565	20.07673970234873
25	24.09254894937432	26.457830475267862	28.5969361103988	20.85268446495902
26	23.284584084652465	27.75355918359594	27.125775232451016	21.836081499300576
27	22.612433297107202	26.728101845633255	28.979277838154665	21.680187019104874
28	20.91523220679559	27.067026485780225	30.42058946837133	21.597151839052856
29	22.263739812248232	25.77061938545826	30.166328149061716	21.79931265323179
30	23.029644373348965	25.693282698154906	30.12209699106015	21.154975937435978
31	25.379865596978167	25.557197264180765	27.4425842935972	21.62035284524386
32	25.909825422604293	25.829910845724164	26.802453608163496	21.457810123508047
33	25.220036443217914	25.61581033245278	26.480624446262535	22.683528778066773
34	22.710800136221113	27.12794608683146	28.63886073562114	21.522393041326286
35	23.744398178110462	27.421418463387866	28.011890854868888	20.822292503632788
36	25.442413338814738	28.328157202419963	25.688805310995242	20.540624147770057
37	23.415785096270607	28.179317998960702	26.872599340331625	21.532297564437066
38	24.146955987284223	28.225041619348822	26.62742847374012	21.00057391962683
39	23.764071545933245	27.146941062660357	26.107237492826002	22.981749898580397
40	26.14794101245935	25.45408168110963	26.91913703111241	21.478840275318607
41	22.222357900621002	25.42708167975285	28.028307941121	24.32225247850515
42	22.85217702774759	25.773604310231374	27.129709906015577	24.244508756005466
43	23.253785088129906	25.857589239074834	27.493735049936436	23.394890622858824
44	22.88921723061393	26.19230784885969	28.51444364394189	22.404031276584487
45	22.33130765483958	26.94762949485575	26.64126767041546	24.079795179889203
46	22.98826246172173	27.23689584105004	27.217493830024814	22.55734786720341
47	22.57417198865186	27.15168980661758	28.714297925341604	21.559840279388958
48	23.34889564567315	26.79309179864783	28.76042858092606	21.097583974752965
49	23.473584094149956	26.445755097776637	27.904569241435638	22.17609156663777
50	22.085594074652967	27.209353126098147	28.741433605097168	21.96361919415172
51	22.13213176543376	27.41164961867586	27.37311695342296	23.08310166246742
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	766.0
1	1070.0
2	1374.0
3	6022.5
4	10671.0
5	7126.0
6	3581.0
7	3584.0
8	3587.0
9	3754.5
10	3922.0
11	3982.5
12	4043.0
13	3997.0
14	3951.0
15	3656.5
16	3362.0
17	3339.0
18	3316.0
19	3331.0
20	3346.0
21	3845.0
22	4344.0
23	4378.5
24	4413.0
25	4685.5
26	5968.5
27	6979.0
28	8482.5
29	9986.0
30	12005.0
31	14024.0
32	15419.5
33	16815.0
34	18940.5
35	21066.0
36	21915.0
37	22764.0
38	24702.0
39	26640.0
40	29740.0
41	32840.0
42	36051.0
43	39262.0
44	40180.5
45	41099.0
46	42856.5
47	44614.0
48	45591.0
49	46568.0
50	47102.0
51	47636.0
52	45967.0
53	44298.0
54	43595.0
55	42892.0
56	41958.5
57	41025.0
58	40238.0
59	39451.0
60	36912.0
61	34373.0
62	31910.5
63	29448.0
64	27492.5
65	25537.0
66	21840.5
67	18144.0
68	15465.0
69	12786.0
70	10837.0
71	8888.0
72	7302.0
73	5716.0
74	4897.0
75	3275.0
76	2472.0
77	1808.5
78	1145.0
79	808.0
80	471.0
81	334.5
82	198.0
83	163.5
84	129.0
85	78.5
86	28.0
87	24.0
88	20.0
89	11.0
90	2.0
91	3.5
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	737037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.420235537254143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.50569828126237	17.92271457028264
2	5.21212095393121	2.6498668459517822
3	2.67505066332923	2.0400125380775087
4	1.971529034032762	2.004669296545918
5	1.598222661454455	2.031359824757472
6	1.3786031394807792	2.1026649910799655
7	1.2601087317336717	2.2422582534254367
8	1.234066237372845	2.5096203538072563
9	1.1229258080419646	2.569053467815936
>10	12.939279934374998	60.28018546451318
>50	0.07183701763398123	1.222133547154092
>100	0.028413148765611587	1.2175001238017962
>500	0.00160829143956292	0.25565594719634765
>1k	0.0	0.0
>5k	5.360971465209733E-4	0.9523047755906596
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6988	0.9481206506593293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03242713730789635	0.0
2	0.0	0.0	0.0	0.10528643745157977	0.0
3	0.0	0.0	0.0	0.15575880179692472	0.0
4	0.0	0.0	0.0	0.20188945738137976	0.0
5	0.0	0.0	0.0	0.33553268017752164	0.0
6	0.0	0.0	0.0	0.47392464693088676	0.0
7	0.0	0.0	0.0	0.5494975150501263	0.0
8	0.0	0.0	0.0	0.6835477730426016	0.0
9	0.0	0.0	0.0	0.7526080780205064	0.0
10	0.0	0.0	0.0	0.8703769281596446	0.0
11	0.0	0.0	0.0	1.0511005553316861	0.0
12	0.0	0.0	0.0	1.1814874965571607	0.0
13	0.0	0.0	0.0	1.2500050879399542	0.0
14	0.0	0.0	0.0	1.286366898812407	0.0
15	0.0	0.0	0.0	1.3396885095320858	0.0
16	0.0	0.0	0.0	1.4244875087682165	0.0
17	0.0	0.0	0.0	1.522175955888239	0.0
18	0.0	0.0	0.0	1.6273267149410409	0.0
19	0.0	0.0	0.0	1.6955729495262788	0.0
20	1.3567839877780898E-4	0.0	0.0	1.7701960688540739	0.0
21	1.3567839877780898E-4	0.0	0.0	1.8564875304767603	0.0
22	1.3567839877780898E-4	0.0	0.0	1.9567538671735611	0.0
23	1.3567839877780898E-4	0.0	0.0	2.064211159005586	0.0
24	1.3567839877780898E-4	0.0	0.0	2.15063829902705	0.0
25	1.3567839877780898E-4	0.0	0.0	2.230824232704735	0.0
26	1.3567839877780898E-4	0.0	0.0	2.307075492817864	0.0
27	1.3567839877780898E-4	0.0	0.0	2.387261426495549	0.0
28	1.3567839877780898E-4	0.0	0.0	2.4689398225597903	0.0
29	1.3567839877780898E-4	0.0	0.0	2.550618218624031	0.0
30	1.3567839877780898E-4	0.0	0.0	2.6616031488242786	0.0
31	2.7135679755561795E-4	0.0	0.0	2.756713706367523	0.0
32	2.7135679755561795E-4	0.0	0.0	2.8523669775058784	0.0
33	2.7135679755561795E-4	0.0	0.0	2.942728791091899	0.0
34	2.7135679755561795E-4	0.0	0.0	3.041638343800922	0.0
35	2.7135679755561795E-4	0.0	0.0	3.157372017958393	0.0
36	2.7135679755561795E-4	0.0	0.0	3.254517751483304	0.0
37	2.7135679755561795E-4	0.0	0.0	3.3579046913519943	0.0
38	2.7135679755561795E-4	0.0	0.0	3.4838142454178014	0.0
39	2.7135679755561795E-4	0.0	0.0	3.6812263156395133	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATA	35	1.2111377E-7	45.0	9
TACGCGG	25	3.8894388E-5	45.0	2
TGCGACG	20	7.0316374E-4	45.0	1
TATTACG	20	7.0316374E-4	45.0	1
TTCGGTA	20	7.0316374E-4	45.0	25
ACCCGTT	20	7.0316374E-4	45.0	25
TACGAAT	90	0.0	42.5	12
CGTTTTT	3225	0.0	41.651165	1
CGTTAGG	105	0.0	40.714287	2
TACGGGT	45	1.9266736E-8	40.0	4
ACACGAC	385	0.0	39.15584	26
GGCGATA	190	0.0	39.078945	8
AATCGTT	75	0.0	39.0	22
CATACGA	290	0.0	38.793106	18
CTAGGGA	530	0.0	38.632076	4
GCGAGAC	405	0.0	38.333336	21
TAACGGG	100	0.0	38.25	3
ATTGGGC	195	0.0	38.076923	4
TAAGGGA	790	0.0	37.87975	4
CGATTGA	30	1.1395244E-4	37.500004	10
>>END_MODULE