FastQCFastQC Report
Thu 26 May 2016
SRR1547729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547729_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1056018
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62790.5945921376340176No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT253.8905888E-545.00000421
CGACGGT650.045.00000428
ACCGCAC253.8905888E-545.00000410
CGCGATA207.0330244E-445.00000436
CCCGTAA207.0330244E-445.00000424
CCGTAAA207.0330244E-445.00000428
CATAGCG207.0330244E-445.00000424
CGTACGA207.0330244E-445.00000438
CGCGTCA207.0330244E-445.00000435
CAATCGA207.0330244E-445.00000413
GTGCGTA207.0330244E-445.00000433
CGATAGT207.0330244E-445.00000412
ATCGATA207.0330244E-445.00000410
ACGCGTC207.0330244E-445.00000411
GTCTACG351.2118107E-745.01
GTTGCGC351.2118107E-745.042
TCCGCGC302.165334E-644.99999631
ACGTACG302.165334E-644.99999637
TGTAGCG1000.042.7500041
CATACGA2450.042.244918