##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547729_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1056018
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.524226859769435	31.0	31.0	33.0	30.0	34.0
2	31.93463653081671	31.0	31.0	34.0	30.0	34.0
3	32.089345067981796	33.0	31.0	34.0	30.0	34.0
4	35.75205536269268	37.0	35.0	37.0	35.0	37.0
5	35.620526354664406	37.0	35.0	37.0	33.0	37.0
6	35.68181129488323	37.0	35.0	37.0	33.0	37.0
7	36.09608832425205	37.0	35.0	37.0	35.0	37.0
8	36.13524769464157	37.0	35.0	37.0	35.0	37.0
9	37.9656066468564	39.0	38.0	39.0	35.0	39.0
10	37.29377245463619	39.0	37.0	39.0	34.0	39.0
11	36.90385391158105	39.0	37.0	39.0	33.0	39.0
12	36.476338471503325	39.0	35.0	39.0	32.0	39.0
13	36.305305401991255	39.0	35.0	39.0	32.0	39.0
14	37.30112649594988	40.0	35.0	41.0	32.0	41.0
15	37.49885323924403	40.0	35.0	41.0	32.0	41.0
16	37.59929281508459	40.0	35.0	41.0	33.0	41.0
17	37.562814270211305	39.0	35.0	41.0	33.0	41.0
18	37.466002473442686	39.0	36.0	41.0	32.0	41.0
19	37.45929520140755	39.0	36.0	41.0	32.0	41.0
20	37.32687226922268	39.0	35.0	41.0	32.0	41.0
21	37.24065025406764	39.0	35.0	41.0	32.0	41.0
22	37.20875022963624	39.0	35.0	41.0	32.0	41.0
23	37.138663356117036	39.0	35.0	41.0	32.0	41.0
24	37.03713667759451	39.0	35.0	41.0	32.0	41.0
25	36.95098568395615	39.0	35.0	41.0	31.0	41.0
26	36.83891467759072	39.0	35.0	41.0	31.0	41.0
27	36.78688052665769	39.0	35.0	41.0	31.0	41.0
28	36.878613811507	39.0	35.0	41.0	31.0	41.0
29	36.871598779566256	39.0	35.0	41.0	31.0	41.0
30	36.888422356437104	39.0	35.0	41.0	31.0	41.0
31	36.76989786158948	39.0	35.0	41.0	31.0	41.0
32	36.658219840949684	39.0	35.0	41.0	31.0	41.0
33	36.601184828288915	39.0	35.0	41.0	30.0	41.0
34	36.45108700798661	39.0	35.0	41.0	30.0	41.0
35	36.3971021327288	39.0	35.0	41.0	30.0	41.0
36	36.269359991969836	39.0	35.0	41.0	30.0	41.0
37	36.065794333051144	38.0	35.0	41.0	29.0	41.0
38	36.1448204481363	38.0	35.0	41.0	30.0	41.0
39	36.07144669882521	38.0	35.0	41.0	30.0	41.0
40	35.966586743786564	38.0	35.0	41.0	29.0	41.0
41	35.91412172898568	38.0	35.0	41.0	29.0	41.0
42	35.91095890410959	38.0	35.0	41.0	29.0	41.0
43	35.86621534860201	38.0	35.0	40.0	29.0	41.0
44	35.74806963517667	38.0	35.0	40.0	29.0	41.0
45	35.70596618618243	38.0	35.0	40.0	29.0	41.0
46	35.53548140277912	38.0	34.0	40.0	28.0	41.0
47	35.583419032630125	38.0	34.0	40.0	28.0	41.0
48	35.42705048588187	38.0	34.0	40.0	28.0	41.0
49	35.425263584522234	38.0	34.0	40.0	28.0	41.0
50	35.30523059266035	37.0	34.0	40.0	28.0	41.0
51	34.60380694268469	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	2.0
14	11.0
15	25.0
16	74.0
17	184.0
18	361.0
19	716.0
20	1195.0
21	1862.0
22	2927.0
23	4140.0
24	6146.0
25	8755.0
26	11817.0
27	14471.0
28	16706.0
29	19522.0
30	23362.0
31	28545.0
32	36030.0
33	46537.0
34	75953.0
35	109345.0
36	75474.0
37	98402.0
38	155638.0
39	317613.0
40	202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.51999492432894	23.172805766568374	30.84095157468907	13.466247734413617
2	28.62280756578013	26.130520502491432	32.121990344861544	13.12468158686689
3	27.87547181960914	25.305913346174023	33.138450291567004	13.680164542649841
4	25.64246063987546	27.86694923760769	31.75239437206563	14.738195750451224
5	23.069871915062055	33.34128774320135	29.69703167938425	13.891808662352345
6	22.216572066006453	41.50469026096146	25.628824508673148	10.649913164358942
7	91.16908992081575	2.750047821154564	4.847265860998581	1.2335963970311112
8	92.48223041652699	1.8565024459810346	4.5467028024143525	1.1145643350776218
9	88.76060824720791	4.245476876341122	5.256065711001138	1.7378491654498314
10	46.44523104719806	36.461405013929685	9.271148787236582	7.822215151635673
11	30.102422496586236	23.87809677486558	27.801230660841007	18.218250067707178
12	31.151647036319456	23.454997926171714	26.859864131103826	18.533490906405003
13	25.488296601004905	27.687880320221815	26.903139908600043	19.920683170173238
14	17.940035113037847	31.163957432543764	29.889736727972437	21.00627072644595
15	18.20499271792716	27.419892463954216	36.23366268378001	18.14145213433862
16	23.42043412138808	26.383451797223152	31.95258035374397	18.243533727644795
17	23.48274366535419	25.51954606834353	27.15644998475405	23.841260281548234
18	24.202333672342707	26.59803147294838	30.43101538041965	18.768619474289263
19	26.740926764505907	27.72575846245045	25.61291568893712	19.92039908410652
20	26.76800963619938	28.306051601393158	27.2549331545485	17.671005607858955
21	25.18839640990968	25.758935927228517	30.734229908959886	18.31843775390192
22	24.78897140010871	21.87121810423686	30.642564804766586	22.697245690887843
23	21.65446043533349	26.385913876468013	30.339539666937494	21.620086021261002
24	21.58571160718851	26.481082708817468	33.11524992945196	18.817955754542062
25	21.741106685681494	28.623754519335844	29.000642034510776	20.634496760471883
26	20.522093373408406	31.75457236524377	25.651456698654755	22.07187756269306
27	19.157627994977357	30.865004195004254	30.122024435189555	19.855343374828838
28	18.72676412712662	28.071585900997896	32.741392665655326	20.460257306220157
29	19.720591883850464	26.665075784693066	31.9058008480916	21.70853148336487
30	21.75739428683981	27.676043400775363	30.375429206699128	20.1911331056857
31	24.910654932018204	26.98997554965919	28.270730233764958	19.82863928455765
32	25.653729387188477	26.90777998102305	27.49545935770034	19.94303127408813
33	24.62997789810401	27.061091761693458	27.45833877831628	20.850591561886255
34	20.367266467048857	27.46364171822829	30.02732907961796	22.141762735104894
35	21.58059805798765	27.351048940453666	29.18037381938565	21.88797918217303
36	25.3913285568996	26.69092761676411	27.757481406566935	20.16026241976936
37	21.210623303769445	29.100450939283235	29.217683789480866	20.471241967466465
38	21.76269722675182	29.057269857142586	27.763257823256797	21.4167750928488
39	21.155984083604633	28.205485133776133	28.514854860428517	22.12367592219072
40	23.33994306915223	25.04616398584115	28.175182619993222	23.4387103250134
41	19.793886089062877	25.44634655848669	29.263137560155222	25.496629792295206
42	21.451717679054713	26.496139270353346	28.2152387554	23.83690429519194
43	21.515447653354393	26.518866155690528	28.312585580927596	23.65310061002748
44	21.35029895323754	27.438736839712956	29.023842396625817	22.187121810423687
45	20.21802658666803	29.64097202888587	26.71838926987987	23.42261211456623
46	22.407383207483207	28.552543611946007	27.73039853487346	21.309674645697328
47	21.07662937563564	26.825300326320196	29.1722300188065	22.925840279237665
48	21.15929842104964	27.54829936610929	30.439632657776666	20.852769555064402
49	22.239393646699206	25.834597516330216	29.562658969828163	22.363349867142414
50	20.89206812762661	27.919126378527636	28.826402580259046	22.362402913586703
51	20.393970557320046	29.495330572016766	28.02613213032354	22.084566740339653
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	631.0
1	1239.5
2	1848.0
3	5644.5
4	9441.0
5	6288.5
6	3136.0
7	3217.0
8	3298.0
9	3427.5
10	3557.0
11	3670.0
12	3783.0
13	3725.0
14	3667.0
15	3399.0
16	3131.0
17	3172.5
18	3214.0
19	3043.5
20	2873.0
21	3491.0
22	4109.0
23	4394.5
24	4680.0
25	6117.0
26	8353.5
27	9153.0
28	12189.5
29	15226.0
30	16789.0
31	18352.0
32	22136.0
33	25920.0
34	30048.0
35	34176.0
36	38171.0
37	42166.0
38	45079.5
39	47993.0
40	55424.0
41	62855.0
42	72395.0
43	81935.0
44	82293.5
45	82652.0
46	84945.5
47	87239.0
48	86279.5
49	85320.0
50	82832.0
51	80344.0
52	75349.5
53	70355.0
54	65876.0
55	61397.0
56	57820.0
57	54243.0
58	48545.0
59	42847.0
60	38374.5
61	33902.0
62	28616.0
63	23330.0
64	19871.5
65	16413.0
66	13587.0
67	10761.0
68	8376.5
69	5992.0
70	4919.5
71	3847.0
72	3028.5
73	2210.0
74	1692.0
75	966.5
76	759.0
77	509.5
78	260.0
79	191.0
80	122.0
81	97.5
82	73.0
83	61.5
84	50.0
85	35.5
86	21.0
87	14.0
88	7.0
89	3.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1056018.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.475666013649924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.48099272709678	15.674209347999254
2	6.779173576568108	2.6405784085160224
3	2.039922393953211	1.1918654171519383
4	1.0507084900192343	0.8185299051728412
5	0.6793927470071944	0.6615813116404139
6	0.4830348621615162	0.5644455389044271
7	0.404138768747227	0.5509610178302087
8	0.35494257069799806	0.5530194368752428
9	0.28101220875734007	0.49256099311644236
>10	4.151804334038298	20.98858997787134
>50	2.4212652521851314	34.173536315572456
>100	0.8711663462910629	20.853512579448935
>500	0.0019565779815633086	0.2384461630609162
>1k	0.0	0.0
>5k	4.891444953908272E-4	0.5981635868395897
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6279	0.5945921376340176	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03655240725063399	0.0
2	0.0	0.0	0.0	0.15994045556041658	0.0
3	0.0	0.0	0.0	0.22802641621639025	0.0
4	0.0	0.0	0.0	0.3429865778803013	0.0
5	0.0	0.0	0.0	0.600747335746171	0.0
6	0.0	0.0	0.0	0.8124861508042477	0.0
7	0.0	0.0	0.0	0.948184595338337	0.0
8	0.0	0.0	0.0	1.2235586893405226	0.0
9	0.0	0.0	0.0	1.3094473768439554	0.0
10	9.469535557159063E-5	0.0	0.0	1.4782891958281015	0.0
11	9.469535557159063E-5	0.0	0.0	1.7653108185655926	0.0
12	9.469535557159063E-5	0.0	0.0	1.9696633958890852	0.0
13	9.469535557159063E-5	0.0	0.0	2.053184699503228	0.0
14	9.469535557159063E-5	0.0	0.0	2.0878431996424305	0.0
15	9.469535557159063E-5	0.0	0.0	2.1460808433189587	0.0
16	9.469535557159063E-5	0.0	0.0	2.2773286061411833	0.0
17	9.469535557159063E-5	0.0	0.0	2.458007344571778	0.0
18	1.8939071114318126E-4	0.0	0.0	2.641905725091807	0.0
19	1.8939071114318126E-4	0.0	0.0	2.7710701900914567	0.0
20	2.840860667147719E-4	0.0	0.0	2.9027914296915394	0.0
21	2.840860667147719E-4	0.0	0.0	3.065572745919104	0.0
22	2.840860667147719E-4	0.0	0.0	3.270682886087169	0.0
23	2.840860667147719E-4	0.0	0.0	3.4681227024539356	0.0
24	2.840860667147719E-4	0.0	0.0	3.6124384243450396	0.0
25	2.840860667147719E-4	0.0	0.0	3.748326259590272	0.0
26	2.840860667147719E-4	0.0	0.0	3.8768278571009205	0.0
27	2.840860667147719E-4	0.0	0.0	4.035063796261048	0.0
28	2.840860667147719E-4	0.0	0.0	4.187428623375738	0.0
29	2.840860667147719E-4	0.0	0.0	4.3697171828510495	0.0
30	2.840860667147719E-4	0.0	0.0	4.562706317505952	0.0
31	2.840860667147719E-4	0.0	0.0	4.732589785401386	0.0
32	2.840860667147719E-4	0.0	0.0	4.8829660100490715	0.0
33	2.840860667147719E-4	0.0	0.0	5.034005102185758	0.0
34	2.840860667147719E-4	0.0	0.0	5.215441403460926	0.0
35	2.840860667147719E-4	0.0	0.0	5.4084305381158275	0.0
36	2.840860667147719E-4	0.0	0.0	5.578692787433548	0.0
37	2.840860667147719E-4	0.0	0.0	5.759845002642001	0.0
38	2.840860667147719E-4	0.0	0.0	5.985219948902386	0.0
39	2.840860667147719E-4	0.0	0.0	6.364474847966607	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	25	3.8905888E-5	45.000004	21
CGACGGT	65	0.0	45.000004	28
ACCGCAC	25	3.8905888E-5	45.000004	10
CGCGATA	20	7.0330244E-4	45.000004	36
CCCGTAA	20	7.0330244E-4	45.000004	24
CCGTAAA	20	7.0330244E-4	45.000004	28
CATAGCG	20	7.0330244E-4	45.000004	24
CGTACGA	20	7.0330244E-4	45.000004	38
CGCGTCA	20	7.0330244E-4	45.000004	35
CAATCGA	20	7.0330244E-4	45.000004	13
GTGCGTA	20	7.0330244E-4	45.000004	33
CGATAGT	20	7.0330244E-4	45.000004	12
ATCGATA	20	7.0330244E-4	45.000004	10
ACGCGTC	20	7.0330244E-4	45.000004	11
GTCTACG	35	1.2118107E-7	45.0	1
GTTGCGC	35	1.2118107E-7	45.0	42
TCCGCGC	30	2.165334E-6	44.999996	31
ACGTACG	30	2.165334E-6	44.999996	37
TGTAGCG	100	0.0	42.750004	1
CATACGA	245	0.0	42.2449	18
>>END_MODULE