FastQCFastQC Report
Thu 26 May 2016
SRR1547728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547728_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2396410
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141330.5897571784460923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAC207.0348196E-445.00000426
CACGCGA207.0348196E-445.00000420
TACTCGC502.1827873E-1145.045
CTCGATA253.892077E-545.039
CGGTCTA1150.043.04347631
CGTTTTT66700.041.660421
AGGGCGA16050.039.6728946
CGACGGT1250.039.60000228
GTCGATA403.460318E-739.37500413
ACGATTG1950.039.230771
TCGTCCC5050.039.20792438
TGCGTAG1900.039.078951
TAGGGCG6700.038.9552235
AGGGATT56400.038.9361726
AATAGCG1850.038.9189221
ACGGGAT13200.038.8636365
CGGGATA6450.038.3720936
ATAGCGG5650.038.230092
ATTAGCG2600.038.0769271
GGGCGAT31700.038.0441637
Produced by FastQC (version 0.11.5)