##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547728_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2396410
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.547730980925635	31.0	31.0	33.0	30.0	34.0
2	31.960823481791515	31.0	31.0	34.0	30.0	34.0
3	32.097193301647046	33.0	31.0	34.0	30.0	34.0
4	35.76755897363139	37.0	35.0	37.0	35.0	37.0
5	35.651891370842215	37.0	35.0	37.0	33.0	37.0
6	35.7112747818612	37.0	35.0	37.0	33.0	37.0
7	36.11660901097892	37.0	35.0	37.0	35.0	37.0
8	36.15281817385172	37.0	35.0	37.0	35.0	37.0
9	37.95881380898928	39.0	38.0	39.0	35.0	39.0
10	37.28387003893324	39.0	37.0	39.0	34.0	39.0
11	36.95650535592824	39.0	37.0	39.0	33.0	39.0
12	36.60282964935049	39.0	35.0	39.0	32.0	39.0
13	36.48277548499631	39.0	35.0	39.0	32.0	39.0
14	37.58288481520274	40.0	36.0	41.0	32.0	41.0
15	37.72742018268994	40.0	36.0	41.0	33.0	41.0
16	37.78702225412179	40.0	36.0	41.0	33.0	41.0
17	37.71095346789573	40.0	36.0	41.0	33.0	41.0
18	37.62229877191299	39.0	36.0	41.0	33.0	41.0
19	37.614482914025565	39.0	36.0	41.0	33.0	41.0
20	37.518202227498634	39.0	35.0	41.0	32.0	41.0
21	37.41972450457142	39.0	35.0	41.0	32.0	41.0
22	37.362150466739834	39.0	35.0	41.0	32.0	41.0
23	37.26919058091061	39.0	35.0	41.0	32.0	41.0
24	37.19739735687966	39.0	35.0	41.0	32.0	41.0
25	37.085585938967036	39.0	35.0	41.0	32.0	41.0
26	36.97569072070305	39.0	35.0	41.0	31.0	41.0
27	36.943499234271265	39.0	35.0	41.0	31.0	41.0
28	36.986934205749435	39.0	35.0	41.0	31.0	41.0
29	36.97473679378737	39.0	35.0	41.0	31.0	41.0
30	36.990618466789904	39.0	35.0	41.0	31.0	41.0
31	36.906049465659045	39.0	35.0	41.0	31.0	41.0
32	36.75509866842485	39.0	35.0	41.0	31.0	41.0
33	36.69716659503173	39.0	35.0	41.0	31.0	41.0
34	36.55961751119383	39.0	35.0	41.0	30.0	41.0
35	36.50327281224832	39.0	35.0	41.0	30.0	41.0
36	36.38588889213448	39.0	35.0	41.0	30.0	41.0
37	36.1717026719134	39.0	35.0	41.0	30.0	41.0
38	36.25161679345354	39.0	35.0	41.0	30.0	41.0
39	36.176901698791106	39.0	35.0	41.0	30.0	41.0
40	36.08646433623629	38.0	35.0	41.0	30.0	41.0
41	36.05109184154631	38.0	35.0	41.0	29.0	41.0
42	36.02425294503028	38.0	35.0	41.0	29.0	41.0
43	35.96683622585451	38.0	35.0	41.0	29.0	41.0
44	35.829643091123806	38.0	35.0	40.0	29.0	41.0
45	35.79884160056084	38.0	35.0	40.0	29.0	41.0
46	35.615957202648964	38.0	34.0	40.0	28.0	41.0
47	35.64320337504851	38.0	35.0	40.0	28.0	41.0
48	35.48680401099979	38.0	34.0	40.0	28.0	41.0
49	35.45963503741012	38.0	34.0	40.0	28.0	41.0
50	35.3492278032557	38.0	34.0	40.0	28.0	41.0
51	34.65436674024895	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	4.0
14	19.0
15	37.0
16	116.0
17	332.0
18	683.0
19	1386.0
20	2455.0
21	3920.0
22	5908.0
23	9048.0
24	13427.0
25	19182.0
26	26030.0
27	31948.0
28	36086.0
29	41810.0
30	49999.0
31	62595.0
32	78577.0
33	102452.0
34	165460.0
35	240108.0
36	176254.0
37	229056.0
38	363163.0
39	735846.0
40	505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.71305411010637	23.64587028096194	29.29252506874867	13.348550540183023
2	30.656106425862017	25.729946044291253	30.500999411619883	13.112948118226846
3	28.974966721053576	25.588275795878	31.95550844805355	13.481249035014875
4	25.500686443471693	28.50129986104214	31.10544522848761	14.89256846699855
5	22.88085093953038	33.44281654641735	29.85006739247458	13.826265121577693
6	22.641325983450244	41.97044746099374	25.69385038453353	9.694376171022489
7	91.90084334483664	2.459428895723186	4.699446255023139	0.9402815044170239
8	93.04459587466252	1.691822350933271	4.376838687870607	0.8867430865336066
9	88.2218818983396	4.744471939275834	5.416769250670795	1.616876911713772
10	43.266302510839125	36.892893953872665	10.659987230899553	9.180816304388648
11	28.26269294486336	24.293630889538935	29.398224844663474	18.04545132093423
12	29.553665691597015	22.936308895389352	29.062097053509206	18.447928359504427
13	24.266298337930486	28.424977361970615	28.647935870739982	18.660788429358917
14	18.852700497828	31.392249239487402	29.6129627234071	20.142087539277505
15	18.515696395858804	27.761693533243477	35.68717373070551	18.035436340192206
16	22.78963115660509	25.767627409333127	33.63243351513305	17.810307918928732
17	22.16740874892026	25.040331162029872	29.235481407605548	23.556778681444328
18	22.525694684966265	25.86514828430861	31.774404212968566	19.83475281775656
19	25.765582684098298	27.04478782846007	27.357630789389127	19.8319986980525
20	26.44163561327152	27.764531111120384	27.867727141849684	17.926106133758413
21	24.466138932820343	26.745965840569852	30.74244390567557	18.04545132093423
22	24.376004106142105	23.986546542536544	29.71073397290113	21.92671537842022
23	21.689568980266316	28.166799504258456	29.174097921474207	20.969533594001028
24	21.38340267316528	26.71124724066416	32.67437541989893	19.23097466627163
25	20.968448637753973	28.597694050684147	30.391544017926815	20.04231329363506
26	20.32331696162176	31.259049995618447	27.308932945531023	21.108700097228773
27	19.93281617085557	30.28638672013554	30.209563472026908	19.571233636981987
28	18.872521813879928	28.579917459867048	32.61678928063228	19.93077144562074
29	19.994742135110435	26.351208682988304	32.539924303437225	21.114124878464036
30	20.68544197361887	28.596734281696374	30.71949290814176	19.998330836542998
31	24.150667039446503	27.374656256650574	28.122524943561412	20.35215176034151
32	24.46676486911672	27.855291874095002	28.382789255594826	19.295154001193453
33	23.5836522131021	27.432784874040756	28.264487295579638	20.71907561727751
34	20.76781519022204	27.647606210957225	29.576992250908653	22.007586347912085
35	21.14304313535664	27.754808233983336	29.945418354955954	21.156730275704074
36	24.864818624525853	27.771625055812656	28.093731873928085	19.26982444573341
37	21.732675126543455	28.939872559370055	29.367011488017493	19.960440826068997
38	21.270316848953225	30.15627542866204	27.901402514594746	20.672005207789987
39	20.860036471221534	29.102490809168714	28.67697931489186	21.36049340471789
40	23.034372248488364	27.230148430360412	28.079752629975673	21.655726691175552
41	20.35369573653924	26.950021073188644	29.167212622214063	23.529070568058053
42	21.59096314904378	27.89752170955721	28.21462103730163	22.29689410409738
43	21.91690904311032	27.894099924470357	28.339975212922663	21.849015819496664
44	20.72345717135215	28.773790795398117	28.818399188786557	21.684352844463177
45	20.562257710491945	30.18769743074015	27.235364566163554	22.014680292604353
46	22.0036220847017	29.745035281942574	27.831673211178387	20.41966942217734
47	21.126351500786594	28.204063578436077	29.323404592703252	21.346180328074077
48	21.60707057640387	27.768286728898644	29.789935778935995	20.834706915761494
49	20.92555113690896	27.145772217608837	30.14876419310552	21.779912452376678
50	20.728339474463887	28.91904974524393	29.479972124970267	20.872638655321918
51	20.23727158541318	29.839718579041147	27.9019867218047	22.02102311374097
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1736.0
1	2720.0
2	3704.0
3	12429.0
4	21154.0
5	14208.5
6	7263.0
7	7469.0
8	7675.0
9	8198.5
10	8722.0
11	8991.0
12	9260.0
13	9267.5
14	9275.0
15	9120.0
16	8965.0
17	9028.5
18	9092.0
19	9080.0
20	9068.0
21	10582.5
22	12097.0
23	12561.0
24	13025.0
25	16049.0
26	23701.0
27	28329.0
28	36291.5
29	44254.0
30	49533.0
31	54812.0
32	61438.0
33	68064.0
34	75978.5
35	83893.0
36	92190.0
37	100487.0
38	111200.0
39	121913.0
40	132894.0
41	143875.0
42	163820.0
43	183765.0
44	183386.5
45	183008.0
46	186176.5
47	189345.0
48	189101.5
49	188858.0
50	177810.5
51	166763.0
52	157903.5
53	149044.0
54	138175.5
55	127307.0
56	118625.5
57	109944.0
58	102471.0
59	94998.0
60	85566.0
61	76134.0
62	63633.5
63	51133.0
64	42821.0
65	34509.0
66	28557.5
67	22606.0
68	17774.5
69	12943.0
70	10861.0
71	8779.0
72	6907.0
73	5035.0
74	4076.5
75	2440.0
76	1762.0
77	1346.5
78	931.0
79	638.5
80	346.0
81	284.5
82	223.0
83	134.0
84	45.0
85	40.5
86	36.0
87	21.0
88	6.0
89	17.5
90	29.0
91	17.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2396410.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.459299319483815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95382924852795	14.13397135908433
2	6.858132887123523	2.394763896981706
3	2.120296756562453	1.1105668715686372
4	1.0275159407326806	0.7175883345917144
5	0.6742878320055365	0.588629654323524
6	0.4730229899329072	0.49551899797414845
7	0.37771232527800847	0.4616214780580888
8	0.29372308157856136	0.41025593586570125
9	0.25152699552848257	0.39523365916760206
>10	3.3301648008828226	14.788293708968508
>50	2.092207041757554	27.01362464195053
>100	1.542271905196559	36.267601289276804
>500	0.004343068548803816	0.4865982843586733
>1k	7.238447581339694E-4	0.1403640796020344
>5k	0.0	0.0
>10k+	2.4128158604465647E-4	0.5953678082279569
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14133	0.5897571784460923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1729086425110896E-5	0.0	0.0	0.03296597827583761	0.0
2	4.1729086425110896E-5	0.0	0.0	0.11099936989079498	0.0
3	4.1729086425110896E-5	0.0	0.0	0.15009952387112388	0.0
4	4.1729086425110896E-5	0.0	0.0	0.21419540062009423	0.0
5	4.1729086425110896E-5	0.0	0.0	0.3606644939722335	0.0
6	4.1729086425110896E-5	0.0	0.0	0.5003317462370797	0.0
7	4.1729086425110896E-5	0.0	0.0	0.5881714731619381	0.0
8	4.1729086425110896E-5	0.0	0.0	0.7792489599025209	0.0
9	4.1729086425110896E-5	0.0	0.0	0.8587845986287822	0.0
10	4.1729086425110896E-5	0.0	0.0	1.0105950150433356	0.0
11	4.1729086425110896E-5	0.0	0.0	1.2736551758672348	0.0
12	4.1729086425110896E-5	0.0	0.0	1.461185690261683	0.0
13	4.1729086425110896E-5	0.0	0.0	1.5359224840490566	0.0
14	4.1729086425110896E-5	0.0	0.0	1.5721433310660529	0.0
15	4.1729086425110896E-5	0.0	0.0	1.6220513184304857	0.0
16	8.345817285022179E-5	0.0	0.0	1.7289195087651945	0.0
17	8.345817285022179E-5	0.0	0.0	1.8736359804874791	0.0
18	8.345817285022179E-5	0.0	0.0	2.020981384654546	0.0
19	8.345817285022179E-5	0.0	0.0	2.121840586544039	0.0
20	2.0864543212555447E-4	0.0	0.0	2.2210723540629527	0.0
21	2.0864543212555447E-4	0.0	0.0	2.351809581832825	0.0
22	2.0864543212555447E-4	0.0	0.0	2.5082936559269906	0.0
23	2.0864543212555447E-4	0.0	0.0	2.668616805972267	0.0
24	2.0864543212555447E-4	0.0	0.0	2.7953480414453287	0.0
25	2.0864543212555447E-4	0.0	0.0	2.905053809656945	0.0
26	2.0864543212555447E-4	0.0	0.0	3.0067893223613655	0.0
27	2.0864543212555447E-4	0.0	0.0	3.1222954335860726	0.0
28	2.0864543212555447E-4	0.0	0.0	3.2373842539465283	0.0
29	2.0864543212555447E-4	0.0	0.0	3.3684553144078015	0.0
30	2.0864543212555447E-4	0.0	0.0	3.521767977933659	0.0
31	2.0864543212555447E-4	0.0	0.0	3.6559269907903906	0.0
32	2.0864543212555447E-4	0.0	0.0	3.780571771942197	0.0
33	2.0864543212555447E-4	0.0	0.0	3.9161913028238073	0.0
34	2.0864543212555447E-4	0.0	0.0	4.048848068569235	0.0
35	2.0864543212555447E-4	0.0	0.0	4.213594501775573	0.0
36	2.0864543212555447E-4	0.0	0.0	4.355932415571626	0.0
37	2.0864543212555447E-4	0.0	0.0	4.504487963245021	0.0
38	2.0864543212555447E-4	0.0	0.0	4.670569727216962	0.0
39	2.0864543212555447E-4	0.0	0.0	4.895239128529759	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAC	20	7.0348196E-4	45.000004	26
CACGCGA	20	7.0348196E-4	45.000004	20
TACTCGC	50	2.1827873E-11	45.0	45
CTCGATA	25	3.892077E-5	45.0	39
CGGTCTA	115	0.0	43.043476	31
CGTTTTT	6670	0.0	41.66042	1
AGGGCGA	1605	0.0	39.672894	6
CGACGGT	125	0.0	39.600002	28
GTCGATA	40	3.460318E-7	39.375004	13
ACGATTG	195	0.0	39.23077	1
TCGTCCC	505	0.0	39.207924	38
TGCGTAG	190	0.0	39.07895	1
TAGGGCG	670	0.0	38.955223	5
AGGGATT	5640	0.0	38.936172	6
AATAGCG	185	0.0	38.918922	1
ACGGGAT	1320	0.0	38.863636	5
CGGGATA	645	0.0	38.372093	6
ATAGCGG	565	0.0	38.23009	2
ATTAGCG	260	0.0	38.076927	1
GGGCGAT	3170	0.0	38.044163	7
>>END_MODULE