FastQCFastQC Report
Thu 26 May 2016
SRR1547727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547727_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1955014
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154950.7925774444581982No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26900.1375949225939047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT302.1662854E-645.00000431
ATCTCGC453.8562575E-1045.035
TATAGCG1050.045.01
CGACTAT207.0345023E-445.016
CGTTTTT83850.042.3971371
TACGCGG1350.041.6666642
ATACCGG501.0822987E-940.52
TAGGGAC18850.039.9867365
ACGGGAT6200.039.9193535
CGACGGT3400.039.70588328
TACGGGA6450.039.4186064
CGACCGG403.4598816E-739.3752
TACGGGT800.039.3754
CTCACGA3850.039.1558424
CACGACG3500.038.57142626
TACGACT356.2505387E-638.57142614
ATAGCGG3100.038.467742
GCGCGAC2050.038.4146359
CGGTCTA3350.038.2835831
TTAGGGA27000.038.1666684
Produced by FastQC (version 0.11.5)