##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547727_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1955014
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61972701985766	31.0	31.0	33.0	30.0	34.0
2	32.001900242146604	33.0	31.0	34.0	30.0	34.0
3	32.039766467145505	33.0	31.0	34.0	30.0	34.0
4	35.75046521405985	37.0	35.0	37.0	35.0	37.0
5	35.684026303647954	37.0	35.0	37.0	33.0	37.0
6	35.74550105523541	37.0	35.0	37.0	33.0	37.0
7	36.11330609397171	37.0	35.0	37.0	35.0	37.0
8	36.01255745483152	37.0	35.0	37.0	35.0	37.0
9	37.813900565417946	39.0	38.0	39.0	35.0	39.0
10	37.42688134202619	39.0	37.0	39.0	35.0	39.0
11	37.091014181995625	39.0	37.0	39.0	33.0	39.0
12	36.39619869729833	38.0	35.0	39.0	33.0	39.0
13	36.137787760087654	38.0	35.0	39.0	32.0	39.0
14	37.06151055695765	39.0	35.0	41.0	32.0	41.0
15	37.316813588035686	39.0	35.0	41.0	32.0	41.0
16	37.448230038250365	39.0	35.0	41.0	33.0	41.0
17	37.3618025241763	39.0	35.0	41.0	33.0	41.0
18	37.26329478970483	39.0	35.0	41.0	32.0	41.0
19	37.20676117920383	39.0	35.0	41.0	32.0	41.0
20	37.07857130434871	39.0	35.0	41.0	32.0	41.0
21	36.934749316373185	38.0	35.0	41.0	32.0	41.0
22	36.90980320345532	38.0	35.0	41.0	32.0	41.0
23	36.831774094712365	38.0	35.0	41.0	32.0	41.0
24	36.81243766029297	38.0	35.0	41.0	32.0	41.0
25	36.72433752392566	38.0	35.0	41.0	32.0	41.0
26	36.68281557063018	38.0	35.0	41.0	32.0	41.0
27	36.58500348335102	38.0	35.0	41.0	31.0	41.0
28	36.584321135296214	38.0	35.0	40.0	31.0	41.0
29	36.513466399729104	38.0	35.0	40.0	31.0	41.0
30	36.49523942028036	38.0	35.0	40.0	31.0	41.0
31	36.32776133572445	38.0	35.0	41.0	31.0	41.0
32	36.12377814174221	38.0	35.0	41.0	30.0	41.0
33	36.00569714590279	38.0	35.0	41.0	30.0	41.0
34	35.80079324240133	38.0	35.0	41.0	29.0	41.0
35	35.685637545306584	38.0	35.0	41.0	29.0	41.0
36	35.5869533415106	38.0	35.0	40.0	29.0	41.0
37	35.31461513830592	38.0	34.0	40.0	27.0	41.0
38	35.421498771875804	38.0	34.0	40.0	28.0	41.0
39	35.33942212178532	38.0	35.0	40.0	27.0	41.0
40	35.207613858519686	38.0	34.0	40.0	27.0	41.0
41	35.14124502433231	37.0	34.0	40.0	26.0	41.0
42	35.16549600156316	37.0	34.0	40.0	27.0	41.0
43	35.07844496254247	37.0	34.0	40.0	26.0	41.0
44	34.941871516009606	37.0	34.0	40.0	26.0	41.0
45	34.9371186088693	37.0	34.0	40.0	26.0	41.0
46	34.7758860038854	37.0	34.0	40.0	25.0	41.0
47	34.67406422665004	36.0	34.0	40.0	24.0	41.0
48	34.526264773551496	36.0	34.0	40.0	24.0	41.0
49	34.60105298478681	36.0	34.0	40.0	25.0	41.0
50	34.474435988693685	36.0	34.0	40.0	24.0	41.0
51	33.87176562418479	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	4.0
13	9.0
14	12.0
15	39.0
16	136.0
17	333.0
18	793.0
19	1518.0
20	2764.0
21	4477.0
22	6774.0
23	9913.0
24	14497.0
25	21207.0
26	28628.0
27	33915.0
28	35681.0
29	38058.0
30	44027.0
31	53303.0
32	66860.0
33	88517.0
34	160034.0
35	267949.0
36	124295.0
37	162489.0
38	261304.0
39	527324.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.66811797767177	24.272102399266707	29.759070779032786	15.300708844028737
2	31.735936417846624	24.880895993583678	30.32980837988884	13.05335920868086
3	28.498465995639926	24.491333565897737	33.14641225075626	13.863788187706072
4	25.844725408616	26.949883734847933	32.38135379081684	14.824037065719223
5	23.46637926889526	31.070774940742112	31.16494306434634	14.297902726016284
6	22.575592809054054	39.9982813422308	27.311773726428555	10.114352122286592
7	87.54546003251129	3.50145830157738	7.479690682521967	1.4733909833893772
8	88.63184611465698	2.5988560695729035	7.161994747863698	1.6073030679064193
9	84.67954705183696	4.674237626942825	8.256462613566962	2.3897527076532445
10	46.0202842537189	33.38983761753113	12.92302766118299	7.6668504675669835
11	38.83854540172091	19.44952823867246	26.480935686394062	15.230990673212572
12	36.40521244349145	19.7069176998221	28.34409369958476	15.543776157101687
13	25.28733809578857	30.81788672613086	27.813100059641517	16.081675118439048
14	16.503308927711004	35.48997603086218	31.157526237663767	16.84918880376304
15	15.531090825948048	26.350552988367347	43.03457673448886	15.083779451195745
16	20.91018274037935	22.737893437080245	40.25024884732284	16.10167497521757
17	21.08030939931888	22.087821365985103	29.37457225370253	27.457296980993483
18	22.41012084823945	25.037365461321503	33.48482414959688	19.06768954084216
19	26.78625319307176	25.78820407424192	27.970950591658166	19.454592141028147
20	27.891923024592153	26.621497339660994	27.83396947541041	17.652610160336447
21	24.385298519601395	25.769227228040307	31.929183115824234	17.91629113653406
22	23.517222894567507	21.70628957132788	31.180288222999934	23.596199311104677
23	19.630805712900266	26.68216186687154	30.906428291562104	22.780604128666088
24	20.153001461882113	24.72202245099012	36.830477940311425	18.29449814681634
25	19.675050920351467	26.10446779409252	34.37627556631308	19.84420571924293
26	18.985081436756975	32.030410012409114	27.881079112476943	21.10342943835696
27	18.468716848063494	31.182641147326823	32.52897421706443	17.819667787545253
28	16.058708530987502	28.680715329915795	37.16484894737327	18.095727191723434
29	17.82432248567018	25.627847166311852	36.47733468916335	20.070495658854618
30	20.31330721928334	27.50195139267545	33.730960494400556	18.45378089364066
31	26.632545853891582	25.569689015014724	29.00915287563158	18.78861225546211
32	27.371977898879496	26.324568519713925	28.96598182928613	17.337471752120447
33	25.60181154712959	26.658069967785398	28.60874653583043	19.131371949254582
34	20.27550697846665	27.314638156043898	30.472569505896118	21.937285359593332
35	19.589220332949022	27.32563551974564	31.34662974280491	21.738514404500428
36	27.362924255273875	24.917468621708082	29.257028338415992	18.462578784602055
37	20.942049519849988	30.188377167631536	30.049708083931883	18.8198652285866
38	20.65422549403738	30.42320924556039	26.813056070186708	22.10950919021552
39	20.435403531637114	28.798412696788873	29.482244116921922	21.28393965465209
40	23.573693078412738	25.682578232176347	28.327674379825414	22.416054309585505
41	18.923547350556056	24.877878112381804	29.61702576042934	26.5815487766328
42	21.21621635446089	26.289888461156803	28.57938613227322	23.91450905210909
43	21.873756402767448	25.22406489160691	29.13263025226418	23.76954845336146
44	20.16092979385314	28.24654964107674	30.464590023396255	21.12793054167387
45	19.507686389969585	32.390560886009	26.37756046759768	21.724192256423738
46	22.645362130399064	29.54127182720942	28.007676671369104	19.805689371022407
47	21.39452709801567	27.736527717960076	28.744755792030137	22.124189391994122
48	21.328696367391743	26.393468282068568	31.160186065163725	21.11764928537596
49	21.807567618441606	24.800845415940756	31.416092160976856	21.975494804640785
50	20.100776771931045	29.37774358649094	29.12833360784117	21.393146033736844
51	18.969787428632227	30.922182654446463	28.124606780309502	21.98342313661181
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1865.0
1	2731.5
2	3598.0
3	14167.5
4	24737.0
5	17026.5
6	9316.0
7	9606.5
8	9897.0
9	10304.5
10	10712.0
11	10709.5
12	10707.0
13	10399.0
14	10091.0
15	9632.0
16	9173.0
17	8418.0
18	7663.0
19	7890.0
20	8117.0
21	8916.0
22	9715.0
23	11064.5
24	12414.0
25	13911.0
26	18377.0
27	21346.0
28	25393.0
29	29440.0
30	32991.5
31	36543.0
32	42138.0
33	47733.0
34	51859.5
35	55986.0
36	61573.0
37	67160.0
38	76653.5
39	86147.0
40	105719.5
41	125292.0
42	144611.0
43	163930.0
44	169744.0
45	175558.0
46	171614.0
47	167670.0
48	162737.0
49	157804.0
50	145905.5
51	134007.0
52	126951.0
53	119895.0
54	110173.0
55	100451.0
56	92198.0
57	83945.0
58	79230.5
59	74516.0
60	64321.0
61	54126.0
62	46877.5
63	39629.0
64	33574.5
65	27520.0
66	21977.0
67	16434.0
68	13701.0
69	10968.0
70	9163.5
71	7359.0
72	5474.0
73	3589.0
74	2978.5
75	1790.0
76	1212.0
77	870.0
78	528.0
79	381.5
80	235.0
81	169.5
82	104.0
83	78.0
84	52.0
85	41.5
86	31.0
87	21.0
88	11.0
89	7.5
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1955014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.2820305404997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4937809633952	17.343531353237694
2	6.143467968008686	2.61490945839485
3	1.9555695218461642	1.248554708640014
4	1.05885006604649	0.901379177736461
5	0.6861627508176721	0.7301468309327491
6	0.5146558019994405	0.6571752297598475
7	0.40149095132503254	0.5981179881483536
8	0.31318922581691244	0.5332242135032791
9	0.28749916035504974	0.5506709319939771
>10	4.3147135536056185	23.824927656653365
>50	2.2771585240580894	34.53675470408482
>100	0.5500823321272134	14.96855985819588
>500	0.002172330384744766	0.32598415967117833
>1k	9.654801709976738E-4	0.3701096549686164
>5k	0.0	0.0
>10k+	2.4137004274941845E-4	0.7959540740789328
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15495	0.7925774444581982	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2690	0.1375949225939047	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0230105769063547E-4	0.0	0.0	0.023733845384227428	0.0
2	1.0230105769063547E-4	0.0	0.0	0.11094549706549416	0.0
3	1.0230105769063547E-4	0.0	0.0	0.16951285259338297	0.0
4	1.0230105769063547E-4	0.0	0.0	0.25114909663051005	0.0
5	1.0230105769063547E-4	0.0	0.0	0.48147992802097583	0.0
6	1.0230105769063547E-4	0.0	0.0	0.7113504046518337	0.0
7	1.0230105769063547E-4	0.0	0.0	0.8727814736876565	0.0
8	1.0230105769063547E-4	0.0	0.0	1.1431120186351607	0.0
9	1.0230105769063547E-4	0.0	0.0	1.2669985994985202	0.0
10	1.0230105769063547E-4	0.0	0.0	1.4634677807933856	0.0
11	1.0230105769063547E-4	0.0	0.0	1.7559465047309124	0.0
12	1.0230105769063547E-4	0.0	0.0	1.9723132417466065	0.0
13	1.0230105769063547E-4	0.0	0.0	2.0544609910721867	0.0
14	1.0230105769063547E-4	0.0	0.0	2.09921770381184	0.0
15	1.0230105769063547E-4	0.0	0.0	2.1542556728494016	0.0
16	1.0230105769063547E-4	0.0	0.0	2.2653546215014315	0.0
17	1.0230105769063547E-4	0.0	0.0	2.400954673470369	0.0
18	1.0230105769063547E-4	0.0	0.0	2.562283441448501	0.0
19	1.0230105769063547E-4	0.0	0.0	2.6614643168795724	5.1150528845317734E-5
20	1.0230105769063547E-4	0.0	0.0	2.771693706541232	5.1150528845317734E-5
21	1.0230105769063547E-4	0.0	0.0	2.9132783703850715	5.1150528845317734E-5
22	1.0230105769063547E-4	0.0	0.0	3.0662184516325715	5.1150528845317734E-5
23	1.0230105769063547E-4	0.0	0.0	3.226984563793405	5.1150528845317734E-5
24	1.0230105769063547E-4	0.0	0.0	3.3450399843683982	5.1150528845317734E-5
25	1.0230105769063547E-4	0.0	0.0	3.44672723571289	5.1150528845317734E-5
26	1.0230105769063547E-4	0.0	0.0	3.550460508211194	5.1150528845317734E-5
27	1.0230105769063547E-4	0.0	0.0	3.6687716814304143	5.1150528845317734E-5
28	1.0230105769063547E-4	0.0	0.0	3.778489565803621	5.1150528845317734E-5
29	1.0230105769063547E-4	0.0	0.0	3.912248198734127	5.1150528845317734E-5
30	1.0230105769063547E-4	0.0	0.0	4.069382623346943	5.1150528845317734E-5
31	1.534515865359532E-4	0.0	0.0	4.212143749354224	5.1150528845317734E-5
32	1.534515865359532E-4	0.0	0.0	4.334905018582987	5.1150528845317734E-5
33	1.534515865359532E-4	0.0	0.0	4.462320985936674	5.1150528845317734E-5
34	1.534515865359532E-4	0.0	0.0	4.600018209588269	5.1150528845317734E-5
35	1.534515865359532E-4	0.0	0.0	4.773674255018123	5.1150528845317734E-5
36	1.534515865359532E-4	0.0	0.0	4.910808822852419	5.1150528845317734E-5
37	1.534515865359532E-4	0.0	0.0	5.055922873186586	5.1150528845317734E-5
38	1.534515865359532E-4	0.0	0.0	5.218939608616614	5.1150528845317734E-5
39	1.534515865359532E-4	0.0	0.0	5.487940239814139	5.1150528845317734E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGT	30	2.1662854E-6	45.000004	31
ATCTCGC	45	3.8562575E-10	45.0	35
TATAGCG	105	0.0	45.0	1
CGACTAT	20	7.0345023E-4	45.0	16
CGTTTTT	8385	0.0	42.397137	1
TACGCGG	135	0.0	41.666664	2
ATACCGG	50	1.0822987E-9	40.5	2
TAGGGAC	1885	0.0	39.986736	5
ACGGGAT	620	0.0	39.919353	5
CGACGGT	340	0.0	39.705883	28
TACGGGA	645	0.0	39.418606	4
CGACCGG	40	3.4598816E-7	39.375	2
TACGGGT	80	0.0	39.375	4
CTCACGA	385	0.0	39.15584	24
CACGACG	350	0.0	38.571426	26
TACGACT	35	6.2505387E-6	38.571426	14
ATAGCGG	310	0.0	38.46774	2
GCGCGAC	205	0.0	38.414635	9
CGGTCTA	335	0.0	38.28358	31
TTAGGGA	2700	0.0	38.166668	4
>>END_MODULE