FastQCFastQC Report
Thu 26 May 2016
SRR1547726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547726_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1860270
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117310.6306073849494965No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25370.1363780526482715No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA19700.10589860611631643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.034412E-445.00000411
ATAGCGC207.034412E-445.00000418
TAGTACG1000.042.751
CGTTTTT64750.042.3590741
TCACGAC4800.041.71875425
TAATGCG1100.040.9090921
TACGGGA6600.040.568184
CACGACG4950.040.45454426
CGACGGT4900.040.40816528
CTCACGA5000.040.0524
CGATTCG451.929402E-840.010
GTTGCGA451.929402E-840.015
GACGGTC5000.039.629
ACGACGG5100.039.26470627
CGTTAGG3750.039.02
AGGGCGC1500.039.06
CGGTCTA5050.038.7623831
TAGGGAC15800.038.7341775
CGCATCG1050.038.5714321
TATCGTT356.250373E-638.57142614
Produced by FastQC (version 0.11.5)