##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547726_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1860270
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61481612884151	31.0	31.0	33.0	30.0	34.0
2	31.99483838367549	33.0	31.0	34.0	30.0	34.0
3	32.05592521515694	33.0	31.0	34.0	30.0	34.0
4	35.762894633574696	37.0	35.0	37.0	35.0	37.0
5	35.69626344562886	37.0	35.0	37.0	33.0	37.0
6	35.75110172179307	37.0	35.0	37.0	33.0	37.0
7	36.10553199266773	37.0	35.0	37.0	35.0	37.0
8	35.99414493595016	37.0	35.0	37.0	35.0	37.0
9	37.784388287721676	39.0	38.0	39.0	35.0	39.0
10	37.404756298816835	39.0	37.0	39.0	35.0	39.0
11	37.08118552683213	39.0	37.0	39.0	33.0	39.0
12	36.4660893311186	39.0	35.0	39.0	33.0	39.0
13	36.25308799260323	39.0	35.0	39.0	32.0	39.0
14	37.259739715202635	39.0	35.0	41.0	32.0	41.0
15	37.45004488595742	39.0	35.0	41.0	33.0	41.0
16	37.566713434071396	39.0	35.0	41.0	33.0	41.0
17	37.448804205841085	39.0	35.0	41.0	33.0	41.0
18	37.35182849801373	39.0	35.0	41.0	32.0	41.0
19	37.32378740720433	39.0	35.0	41.0	32.0	41.0
20	37.2227300338123	39.0	35.0	41.0	32.0	41.0
21	37.08443881802104	39.0	35.0	41.0	32.0	41.0
22	37.03796169373263	39.0	35.0	41.0	32.0	41.0
23	36.95789912217044	38.0	35.0	41.0	32.0	41.0
24	36.96452719228929	38.0	35.0	41.0	32.0	41.0
25	36.88054207184979	38.0	35.0	41.0	32.0	41.0
26	36.848697769678594	38.0	35.0	41.0	32.0	41.0
27	36.7468684653303	38.0	35.0	41.0	32.0	41.0
28	36.74672816311611	38.0	35.0	41.0	32.0	41.0
29	36.67876491046999	38.0	35.0	41.0	31.0	41.0
30	36.69149693324087	38.0	35.0	41.0	31.0	41.0
31	36.55587307218845	38.0	35.0	41.0	31.0	41.0
32	36.37272331435759	38.0	35.0	41.0	31.0	41.0
33	36.28098555586017	38.0	35.0	41.0	30.0	41.0
34	36.10724787262064	38.0	35.0	41.0	30.0	41.0
35	36.002425991925904	38.0	35.0	41.0	30.0	41.0
36	35.91698516881958	38.0	35.0	41.0	30.0	41.0
37	35.66332091578104	38.0	35.0	41.0	29.0	41.0
38	35.776996887548584	38.0	35.0	41.0	29.0	41.0
39	35.700080095900056	38.0	35.0	40.0	29.0	41.0
40	35.59053793266569	38.0	35.0	40.0	28.0	41.0
41	35.53872502378687	38.0	35.0	40.0	28.0	41.0
42	35.55958059851527	38.0	35.0	40.0	29.0	41.0
43	35.47075424535148	38.0	35.0	40.0	28.0	41.0
44	35.34560950829718	38.0	34.0	40.0	27.0	41.0
45	35.33552817601746	38.0	34.0	40.0	28.0	41.0
46	35.14408338574508	37.0	34.0	40.0	27.0	41.0
47	35.041712762125925	37.0	34.0	40.0	27.0	41.0
48	34.91797319743908	37.0	34.0	40.0	26.0	41.0
49	34.977507566106	37.0	34.0	40.0	27.0	41.0
50	34.83001177248464	37.0	34.0	40.0	26.0	41.0
51	34.232598493767036	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	3.0
12	8.0
13	15.0
14	19.0
15	61.0
16	123.0
17	326.0
18	680.0
19	1369.0
20	2303.0
21	3642.0
22	5901.0
23	8697.0
24	12589.0
25	18250.0
26	24656.0
27	28286.0
28	30914.0
29	34120.0
30	39724.0
31	48559.0
32	60986.0
33	81263.0
34	146081.0
35	242447.0
36	118753.0
37	157534.0
38	258332.0
39	534472.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.8773242593817	23.513575986281563	29.453412676654466	14.155687077682272
2	30.985502104533214	24.843221682873992	30.60442838942734	13.566847823165453
3	29.359555333365588	24.446505077219975	32.54941486988448	13.64452471952996
4	26.873142070774676	26.69687733501051	31.082100985340837	15.34787960887398
5	24.02489961134674	31.60729356491262	29.945975584189394	14.421831239551247
6	22.43298015879415	40.54836125938708	26.56205819585329	10.456600385965478
7	87.41075220263725	3.8134786885774643	6.684567293994957	2.091201814790326
8	88.20380912448194	3.4036457073435575	6.66392512914792	1.728620039026593
9	84.54003988668312	5.381584393663285	7.450746397028388	2.6276293226252103
10	46.06954904395599	33.25952684287765	12.133292479048741	8.537631634117627
11	36.58796841318733	23.270439237315014	25.736049068146023	14.405543281351632
12	35.00857402420079	22.055830605234725	27.191536712412713	15.744058658151774
13	24.79860450364732	31.86032135120171	27.05892155439802	16.282152590752954
14	16.681556978288096	35.98230364409468	29.538239072822766	17.797900304794464
15	15.613271191816242	28.61514726356927	40.571099894101394	15.200481650513098
16	21.32464642229354	25.31578749321335	37.89939094862574	15.460175135867374
17	20.91051299004983	24.984168964720173	28.581066189316605	25.524251855913388
18	22.33229584952722	27.126653657802365	32.4507732748472	18.09027721782322
19	25.937955243056116	28.09113730802518	27.515898229826853	18.455009219091853
20	27.924172297569704	28.362442011105916	27.118805334709478	16.594580356614898
21	24.6021276481371	27.34807312916942	30.873152821902195	17.17664640079128
22	24.067312809430888	23.367898208324597	30.139173345804643	22.425615636439872
23	19.862116789498298	27.822036586086966	30.6669461959823	21.648900428432434
24	20.17105043891478	27.06897385863342	35.366049014390384	17.39392668806141
25	18.890429883834067	28.63998236815086	33.326452611717656	19.143135136297417
26	18.446032027608897	33.78138657291683	27.779999677466172	19.992581722008097
27	17.697377262440398	33.41160154171169	31.022324716304624	17.868696479543292
28	16.33241411192999	30.288345240207065	35.36475887908745	18.0144817687755
29	17.062523182118724	27.177614002268484	35.64729851043128	20.112564305181508
30	19.233122073677478	30.1464841125213	32.439753369134586	18.180640444666636
31	25.575373467292383	27.896004343455523	28.16564262176996	18.362979567482142
32	25.963757949114914	27.646793207437632	29.021539883995334	17.367908959452123
33	25.16543297478323	28.279658329167273	27.63985873018433	18.91504996586517
34	20.295709762561348	28.869250162610804	29.574040327479345	21.260999747348503
35	19.700849876630812	28.46011600466599	31.213909808791197	20.625124309912003
36	25.64616964204121	27.377477462948928	29.04067689098894	17.935676004020923
37	20.411714428550695	31.07091981271536	29.806855994022374	18.710509764711574
38	20.12535814693566	32.0799131308896	26.826320910405478	20.96840781176926
39	19.957855580103963	31.443930182177855	29.036430195616763	19.561784042101415
40	22.71922892913394	27.480849554096988	27.781343568406736	22.018577948362335
41	18.800012901353032	26.516473415149417	29.83072349712676	24.852790186370797
42	21.01194987824348	26.829062447924223	27.816177221586113	24.34281045224618
43	20.982706811376843	27.041773505996442	28.368838931982992	23.606680750643726
44	19.578985846140615	30.1335827594919	29.10185080660334	21.185580587764143
45	18.930209055674712	34.13267966477984	25.939836690372903	20.99727458917254
46	21.40635499147973	31.814575303584963	27.107355383895886	19.671714321039417
47	20.296032296387086	29.500717637762257	28.474683782461685	21.728566283388968
48	21.558322179038527	27.927505147102305	30.313072833513417	20.20109984034576
49	21.634010116811	26.552597203631727	30.742634133754777	21.07075854580249
50	19.751111397807843	30.48874625726373	29.539905497589057	20.220236847339365
51	18.801840593032193	32.18097372961989	27.404194014847306	21.612991662500605
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1781.0
1	2443.5
2	3106.0
3	11136.0
4	19166.0
5	13439.0
6	7712.0
7	7957.0
8	8202.0
9	8442.5
10	8683.0
11	8813.5
12	8944.0
13	8764.5
14	8585.0
15	8261.0
16	7937.0
17	7854.0
18	7771.0
19	7520.0
20	7269.0
21	7953.5
22	8638.0
23	10975.5
24	13313.0
25	15354.0
26	21204.5
27	25014.0
28	29065.5
29	33117.0
30	38682.0
31	44247.0
32	52050.0
33	59853.0
34	61844.0
35	63835.0
36	71913.5
37	79992.0
38	85837.0
39	91682.0
40	107912.5
41	124143.0
42	144385.5
43	164628.0
44	166152.0
45	167676.0
46	166622.5
47	165569.0
48	154370.5
49	143172.0
50	131830.5
51	120489.0
52	110240.0
53	99991.0
54	90674.5
55	81358.0
56	75535.0
57	69712.0
58	64110.0
59	58508.0
60	51946.0
61	45384.0
62	40183.0
63	34982.0
64	29036.0
65	23090.0
66	18962.0
67	14834.0
68	11890.0
69	8946.0
70	7174.0
71	5402.0
72	4149.0
73	2896.0
74	2080.5
75	1051.0
76	837.0
77	777.5
78	718.0
79	455.5
80	193.0
81	157.0
82	121.0
83	85.0
84	49.0
85	34.0
86	19.0
87	20.0
88	21.0
89	14.0
90	7.0
91	5.5
92	4.0
93	6.0
94	8.0
95	5.5
96	3.0
97	1.5
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1860270.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.695910272661386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.30397714157482	16.210517488636313
2	6.298401973666174	2.4810552026896464
3	1.8399010526535382	1.0871557813091797
4	0.9191664375913993	0.7241527872176806
5	0.6366894431433459	0.6270089071851042
6	0.4583028056946452	0.5416014563202419
7	0.3534954978863002	0.48736909257108324
8	0.29765098885242625	0.4690005735205057
9	0.24146733775232987	0.428032711633348
>10	3.603741747430742	18.033334497656135
>50	1.890917598882311	27.18330929158296
>100	1.1538237097113402	30.62386266685487
>500	0.001369036200344668	0.15553074088649593
>1k	8.214217202068009E-4	0.3154297168672381
>5k	0.0	0.0
>10k+	2.7380724006893363E-4	0.6326390850691692
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11731	0.6306073849494965	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2537	0.1363780526482715	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	1970	0.10589860611631643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022684879076693167	0.0
2	0.0	0.0	0.0	0.07353771226757407	0.0
3	0.0	0.0	0.0	0.10170566638176179	0.0
4	0.0	0.0	0.0	0.15433243561418503	0.0
5	0.0	0.0	0.0	0.28399103355964456	0.0
6	0.0	0.0	0.0	0.40300601525584995	0.0
7	0.0	0.0	0.0	0.49831476076053477	0.0
8	0.0	0.0	0.0	0.6845780451224822	0.0
9	0.0	0.0	0.0	0.748869787718987	0.0
10	0.0	0.0	0.0	0.879549742779274	0.0
11	0.0	0.0	0.0	1.0500088696802077	0.0
12	0.0	0.0	0.0	1.182355249506792	0.0
13	0.0	0.0	0.0	1.2419702516301396	0.0
14	0.0	0.0	0.0	1.2638487961424953	0.0
15	0.0	0.0	0.0	1.3094335768463718	0.0
16	5.375563762249566E-5	0.0	0.0	1.3825412440129659	0.0
17	5.375563762249566E-5	0.0	0.0	1.474624651260301	0.0
18	5.375563762249566E-5	0.0	0.0	1.5835335730834772	0.0
19	5.375563762249566E-5	0.0	0.0	1.6658872099211406	0.0
20	5.375563762249566E-5	0.0	0.0	1.752165008305246	0.0
21	5.375563762249566E-5	0.0	0.0	1.85865492643541	0.0
22	5.375563762249566E-5	0.0	0.0	1.9756271939019605	0.0
23	5.375563762249566E-5	0.0	0.0	2.097168690566423	0.0
24	5.375563762249566E-5	0.0	0.0	2.1879082068731956	0.0
25	5.375563762249566E-5	0.0	0.0	2.2810129712353584	0.0
26	5.375563762249566E-5	0.0	0.0	2.362076472770082	0.0
27	5.375563762249566E-5	0.0	0.0	2.4471716471264924	0.0
28	5.375563762249566E-5	0.0	0.0	2.53995387766292	0.0
29	5.375563762249566E-5	0.0	0.0	2.645314927403011	0.0
30	5.375563762249566E-5	0.0	0.0	2.7684153375585265	0.0
31	5.375563762249566E-5	0.0	0.0	2.87641041354212	0.0
32	5.375563762249566E-5	0.0	0.0	2.9872007826820837	0.0
33	5.375563762249566E-5	0.0	0.0	3.092400565509308	0.0
34	5.375563762249566E-5	0.0	0.0	3.2137807952609028	0.0
35	5.375563762249566E-5	0.0	0.0	3.346879754014202	0.0
36	5.375563762249566E-5	0.0	0.0	3.4634757320173954	0.0
37	5.375563762249566E-5	0.0	0.0	3.589640213517393	0.0
38	5.375563762249566E-5	0.0	0.0	3.7312863186526686	0.0
39	5.375563762249566E-5	0.0	0.0	3.927279373424288	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.034412E-4	45.000004	11
ATAGCGC	20	7.034412E-4	45.000004	18
TAGTACG	100	0.0	42.75	1
CGTTTTT	6475	0.0	42.359074	1
TCACGAC	480	0.0	41.718754	25
TAATGCG	110	0.0	40.909092	1
TACGGGA	660	0.0	40.56818	4
CACGACG	495	0.0	40.454544	26
CGACGGT	490	0.0	40.408165	28
CTCACGA	500	0.0	40.05	24
CGATTCG	45	1.929402E-8	40.0	10
GTTGCGA	45	1.929402E-8	40.0	15
GACGGTC	500	0.0	39.6	29
ACGACGG	510	0.0	39.264706	27
CGTTAGG	375	0.0	39.0	2
AGGGCGC	150	0.0	39.0	6
CGGTCTA	505	0.0	38.76238	31
TAGGGAC	1580	0.0	38.734177	5
CGCATCG	105	0.0	38.57143	21
TATCGTT	35	6.250373E-6	38.571426	14
>>END_MODULE