FastQCFastQC Report
Thu 26 May 2016
SRR1547725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547725_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2256484
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103080.4568168885753234No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA302.1664346E-645.00000431
GTCGAAA207.0347317E-445.00000422
CTACGCG207.0347317E-445.0000041
CGTAACG207.0347317E-445.00000417
CTACCGT253.892005E-545.028
ATGCGAT253.892005E-545.032
CACGACG10200.044.1176526
TACGCGG1700.043.6764682
CGGTCTA10200.043.45588331
CGACGGT10350.043.0434828
AACGTAG850.042.352941
CGTTTTT69350.042.015141
ACGGTCT10650.041.83098630
TAACGCG1200.041.2500041
CGAAAGG3550.041.1971862
TCACGAC11050.040.927625
TCGTTAG556.184564E-1140.9090921
TAGTACG556.184564E-1140.9090921
GACGGTC10900.040.87155529
TACGGGA8100.040.5555574
Produced by FastQC (version 0.11.5)