FastQCFastQC Report
Thu 26 May 2016
SRR1547724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547724_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2977702
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142320.4779524613275607No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC40090.13463402314939507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG253.8923063E-545.028
CCGTAAG406.8212103E-945.01
CGCAATA207.0350966E-445.021
CGACTCG207.0350966E-445.018
CGAATCG302.166671E-644.99999620
CGACGGT6150.041.70731728
CGTTTTT99350.041.5802731
CACGACG6200.041.37096826
TCGTTAG1850.041.3513531
CGTTAGG4850.041.2886622
CGGTCTA6300.040.71428731
GATACGA501.0822987E-940.524
ACGGTCT6350.040.393730
CGGGTCA4300.039.244196
TACGGGA8900.038.9325834
CCGCTCG700.038.57142619
GTAGGGT24800.038.1955644
CTAGACG1300.038.0769271
GGGCGAT22050.037.9591837
CGTAAGG4000.037.68752
Produced by FastQC (version 0.11.5)